Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i>
The antimicrobial properties of baicalin against <i>H. pylori</i> and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chrom...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/15/11906 |
| Summary: | The antimicrobial properties of baicalin against <i>H. pylori</i> and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5–8.0 were tested. The test objects were: suspensions of <i>H. pylori</i>, <i>Lactobacillus casei</i>, <i>L. brevis</i>, <i>Bifidobacterium longum</i>, and <i>B. teenis</i>. It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against <i>H. pylori</i> was observed at pH 1.5–2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of <i>L. casei</i> biomass at pH 1.5–2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by <i>H. pylori</i>. |
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| ISSN: | 1661-6596 1422-0067 |