Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i>
The antimicrobial properties of baicalin against <i>H. pylori</i> and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chrom...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/11906 |
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| author | Anastasia Dmitrieva Oksana Kozlova Victor Atuchin Irina Milentieva Anna Vesnina Svetlana Ivanova Lyudmila Asyakina Alexander Prosekov |
| author_facet | Anastasia Dmitrieva Oksana Kozlova Victor Atuchin Irina Milentieva Anna Vesnina Svetlana Ivanova Lyudmila Asyakina Alexander Prosekov |
| author_sort | Anastasia Dmitrieva |
| collection | DOAJ |
| description | The antimicrobial properties of baicalin against <i>H. pylori</i> and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5–8.0 were tested. The test objects were: suspensions of <i>H. pylori</i>, <i>Lactobacillus casei</i>, <i>L. brevis</i>, <i>Bifidobacterium longum</i>, and <i>B. teenis</i>. It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against <i>H. pylori</i> was observed at pH 1.5–2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of <i>L. casei</i> biomass at pH 1.5–2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by <i>H. pylori</i>. |
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| id | doaj.art-f243646fb9ef47edb0bf608e972b75f6 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:26:55Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f243646fb9ef47edb0bf608e972b75f62023-11-18T22:57:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124151190610.3390/ijms241511906Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i>Anastasia Dmitrieva0Oksana Kozlova1Victor Atuchin2Irina Milentieva3Anna Vesnina4Svetlana Ivanova5Lyudmila Asyakina6Alexander Prosekov7Laboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, RussiaDepartment of Bionanotechnology, Kemerovo State University, 650043 Kemerovo, RussiaLaboratory of Optical Materials and Structures, Institute of Semiconductor Physics, 630090 Novosibirsk, RussiaLaboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, RussiaLaboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, RussiaLaboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, RussiaLaboratory of Natural Nutraceuticals Biotesting, Kemerovo State University, 650043 Kemerovo, RussiaLaboratory of Biocatalysis, Kemerovo State University, 650043 Kemerovo, RussiaThe antimicrobial properties of baicalin against <i>H. pylori</i> and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5–8.0 were tested. The test objects were: suspensions of <i>H. pylori</i>, <i>Lactobacillus casei</i>, <i>L. brevis</i>, <i>Bifidobacterium longum</i>, and <i>B. teenis</i>. It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against <i>H. pylori</i> was observed at pH 1.5–2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of <i>L. casei</i> biomass at pH 1.5–2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by <i>H. pylori</i>.https://www.mdpi.com/1422-0067/24/15/11906<i>Scutellaria baicalensis</i>baicalin<i>Helicobacter pylori</i>antimicrobial activitylactic acid bacteriasymbiosis |
| spellingShingle | Anastasia Dmitrieva Oksana Kozlova Victor Atuchin Irina Milentieva Anna Vesnina Svetlana Ivanova Lyudmila Asyakina Alexander Prosekov Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> International Journal of Molecular Sciences <i>Scutellaria baicalensis</i> baicalin <i>Helicobacter pylori</i> antimicrobial activity lactic acid bacteria symbiosis |
| title | Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> |
| title_full | Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> |
| title_fullStr | Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> |
| title_full_unstemmed | Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> |
| title_short | Study of the Effect of Baicalin from <i>Scutellaria baicalensis</i> on the Gastrointestinal Tract Normoflora and <i>Helicobacter pylori</i> |
| title_sort | study of the effect of baicalin from i scutellaria baicalensis i on the gastrointestinal tract normoflora and i helicobacter pylori i |
| topic | <i>Scutellaria baicalensis</i> baicalin <i>Helicobacter pylori</i> antimicrobial activity lactic acid bacteria symbiosis |
| url | https://www.mdpi.com/1422-0067/24/15/11906 |
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