Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria
The growing emergence of microbes resistant to commercially available antibiotic therapies poses a threat to healthcare systems worldwide. Multiple factors have been associated with the increasing incidence of hospital-acquired infections caused by antibiotic-resistant pathogens, including the indis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/16/8/1059 |
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| author | Fabián Espitia-Almeida Roger Valle-Molinares Elkin Navarro Quiroz Leonardo C. Pacheco-Londoño Nataly J. Galán-Freyle |
| author_facet | Fabián Espitia-Almeida Roger Valle-Molinares Elkin Navarro Quiroz Leonardo C. Pacheco-Londoño Nataly J. Galán-Freyle |
| author_sort | Fabián Espitia-Almeida |
| collection | DOAJ |
| description | The growing emergence of microbes resistant to commercially available antibiotic therapies poses a threat to healthcare systems worldwide. Multiple factors have been associated with the increasing incidence of hospital-acquired infections caused by antibiotic-resistant pathogens, including the indiscriminate use of broad-spectrum antibiotics, the massive application of antibiotics in hospitals as a prophylactic measure, self-medication, and nonadherence to pharmacological therapies by patients. In this study, we developed a novel treatment to mitigate the impact of microbial resistance. We synthesized a benzoporphyrin derivative, 5,10,15,20-tetrakis (4-ethylphenyl) porphyrin (TEtPP), with a reaction yield close to 50%. TEtPP exhibited excellent photophysical properties (Φ<sub>f</sub> = 0.12 ± 0.04 and Φ<sub>Δ</sub> = 0.81 ± 0.23) and was thereby assessed as a potential agent for antibacterial photodynamic therapy. The photophysical properties of the synthesized porphyrin derivative were correlated with the assayed antimicrobial activity. TEtPP showed higher activity against the MRSA strain under irradiation than in the absence of irradiation (minimum inhibitory concentration (MIC) = 69.42 µg/mL vs. MIC = 109.30 µg/mL, <i>p <</i> 0.0001). Similar behavior was observed against <i>P. aeruginosa</i> (irradiated MIC = 54.71 µg/mL vs. nonirradiated MIC = 402.90 µg/mL, <i>p <</i> 0.0001). TEtPP exhibited high activity against <i>S. aureus</i> in both the irradiated and nonirradiated assays (MIC = 67.68 µg/mL vs. MIC = 58.26 µg/mL, <i>p =</i> 0.87). |
| first_indexed | 2024-03-10T23:40:04Z |
| format | Article |
| id | doaj.art-f249237adabc46488bd189d803d0f950 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T23:40:04Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-f249237adabc46488bd189d803d0f9502023-11-19T02:33:19ZengMDPI AGPharmaceuticals1424-82472023-07-01168105910.3390/ph16081059Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important BacteriaFabián Espitia-Almeida0Roger Valle-Molinares1Elkin Navarro Quiroz2Leonardo C. Pacheco-Londoño3Nataly J. Galán-Freyle4Life Science Research Center, Universidad Simón Bolívar, Barranquilla 080002, ColombiaFaculty of Basic Sciences, Biology Program, Universidad del Atlántico, Puerto Colombia 081001, ColombiaLife Science Research Center, Universidad Simón Bolívar, Barranquilla 080002, ColombiaLife Science Research Center, Universidad Simón Bolívar, Barranquilla 080002, ColombiaLife Science Research Center, Universidad Simón Bolívar, Barranquilla 080002, ColombiaThe growing emergence of microbes resistant to commercially available antibiotic therapies poses a threat to healthcare systems worldwide. Multiple factors have been associated with the increasing incidence of hospital-acquired infections caused by antibiotic-resistant pathogens, including the indiscriminate use of broad-spectrum antibiotics, the massive application of antibiotics in hospitals as a prophylactic measure, self-medication, and nonadherence to pharmacological therapies by patients. In this study, we developed a novel treatment to mitigate the impact of microbial resistance. We synthesized a benzoporphyrin derivative, 5,10,15,20-tetrakis (4-ethylphenyl) porphyrin (TEtPP), with a reaction yield close to 50%. TEtPP exhibited excellent photophysical properties (Φ<sub>f</sub> = 0.12 ± 0.04 and Φ<sub>Δ</sub> = 0.81 ± 0.23) and was thereby assessed as a potential agent for antibacterial photodynamic therapy. The photophysical properties of the synthesized porphyrin derivative were correlated with the assayed antimicrobial activity. TEtPP showed higher activity against the MRSA strain under irradiation than in the absence of irradiation (minimum inhibitory concentration (MIC) = 69.42 µg/mL vs. MIC = 109.30 µg/mL, <i>p <</i> 0.0001). Similar behavior was observed against <i>P. aeruginosa</i> (irradiated MIC = 54.71 µg/mL vs. nonirradiated MIC = 402.90 µg/mL, <i>p <</i> 0.0001). TEtPP exhibited high activity against <i>S. aureus</i> in both the irradiated and nonirradiated assays (MIC = 67.68 µg/mL vs. MIC = 58.26 µg/mL, <i>p =</i> 0.87).https://www.mdpi.com/1424-8247/16/8/1059photodynamicantimicrobial activityporphyrin<i>Pseudomonas aeruginosa</i>MRSA<i>Staphylococcus aureus</i> |
| spellingShingle | Fabián Espitia-Almeida Roger Valle-Molinares Elkin Navarro Quiroz Leonardo C. Pacheco-Londoño Nataly J. Galán-Freyle Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria Pharmaceuticals photodynamic antimicrobial activity porphyrin <i>Pseudomonas aeruginosa</i> MRSA <i>Staphylococcus aureus</i> |
| title | Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria |
| title_full | Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria |
| title_fullStr | Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria |
| title_full_unstemmed | Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria |
| title_short | Photodynamic Antimicrobial Activity of a Novel 5,10,15,20-Tetrakis (4-Ethylphenyl) Porphyrin against Clinically Important Bacteria |
| title_sort | photodynamic antimicrobial activity of a novel 5 10 15 20 tetrakis 4 ethylphenyl porphyrin against clinically important bacteria |
| topic | photodynamic antimicrobial activity porphyrin <i>Pseudomonas aeruginosa</i> MRSA <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/1424-8247/16/8/1059 |
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