Expression of RBMS3 in Breast Cancer Progression
The aim of the study was to evaluate the localization and intensity of RNA-binding motif single-stranded-interacting protein 3 (RBMS3) expression in clinical material using immunohistochemical (IHC) reactions in cases of ductal breast cancer (in vivo), and to determine the level of RBMS3 expression...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2866 |
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| author | Tomasz Górnicki Jakub Lambrinow Monika Mrozowska Hanna Romanowicz Beata Smolarz Aleksandra Piotrowska Agnieszka Gomułkiewicz Marzena Podhorska-Okołów Piotr Dzięgiel Jędrzej Grzegrzółka |
| author_facet | Tomasz Górnicki Jakub Lambrinow Monika Mrozowska Hanna Romanowicz Beata Smolarz Aleksandra Piotrowska Agnieszka Gomułkiewicz Marzena Podhorska-Okołów Piotr Dzięgiel Jędrzej Grzegrzółka |
| author_sort | Tomasz Górnicki |
| collection | DOAJ |
| description | The aim of the study was to evaluate the localization and intensity of RNA-binding motif single-stranded-interacting protein 3 (RBMS3) expression in clinical material using immunohistochemical (IHC) reactions in cases of ductal breast cancer (in vivo), and to determine the level of RBMS3 expression at both the protein and mRNA levels in breast cancer cell lines (in vitro). Moreover, the data obtained in the in vivo and in vitro studies were correlated with the clinicopathological profiles of the patients. Material for the IHC studies comprised 490 invasive ductal carcinoma (IDC) cases and 26 mastopathy tissues. Western blot and RT-qPCR were performed on four breast cancer cell lines (MCF-7, BT-474, SK-BR-3 and MDA-MB-231) and the HME1-hTERT (Me16C) normal immortalized breast epithelial cell line (control). The Kaplan–Meier plotter tool was employed to analyze the predictive value of overall survival of <i>RBMS3</i> expression at the mRNA level. Cytoplasmatic RBMS3 IHC expression was observed in breast cancer cells and stromal cells. The statistical analysis revealed a significantly decreased RBMS3 expression in the cancer specimens when compared with the mastopathy tissues (<i>p</i> < 0.001). An increased expression of RBMS3 was corelated with HER2(+) cancer specimens (<i>p</i> < 0.05) and ER(−) cancer specimens (<i>p</i> < 0.05). In addition, a statistically significant higher expression of RBMS3 was observed in cancer stromal cells in comparison to the control and cancer cells (<i>p</i> < 0.0001). The statistical analysis demonstrated a significantly higher expression of <i>RBMS3</i> mRNA in the SK-BR-3 cell line compared with all other cell lines (<i>p</i> < 0.05). A positive correlation was revealed between the expression of RBMS3, at both the mRNA and protein levels, and longer overall survival. The differences in the expression of RBMS3 in cancer cells (both in vivo and in vitro) and the stroma of breast cancer with regard to the molecular status of the tumor may indicate that RBMS3 could be a potential novel target for the development of personalized methods of treatment. RBMS3 can be an indicator of longer overall survival for potential use in breast cancer diagnostic process. |
| first_indexed | 2024-03-11T09:40:24Z |
| format | Article |
| id | doaj.art-f24a6d36cc1e4e178edb0631ec032177 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:24Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f24a6d36cc1e4e178edb0631ec0321772023-11-16T17:03:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01243286610.3390/ijms24032866Expression of RBMS3 in Breast Cancer ProgressionTomasz Górnicki0Jakub Lambrinow1Monika Mrozowska2Hanna Romanowicz3Beata Smolarz4Aleksandra Piotrowska5Agnieszka Gomułkiewicz6Marzena Podhorska-Okołów7Piotr Dzięgiel8Jędrzej Grzegrzółka9Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandLaboratory of Cancer Genetics, Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, Rzgowska 281/289, 93-338 Lodz, PolandLaboratory of Cancer Genetics, Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, Rzgowska 281/289, 93-338 Lodz, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Ultrastructure Research, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandDivision of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, PolandThe aim of the study was to evaluate the localization and intensity of RNA-binding motif single-stranded-interacting protein 3 (RBMS3) expression in clinical material using immunohistochemical (IHC) reactions in cases of ductal breast cancer (in vivo), and to determine the level of RBMS3 expression at both the protein and mRNA levels in breast cancer cell lines (in vitro). Moreover, the data obtained in the in vivo and in vitro studies were correlated with the clinicopathological profiles of the patients. Material for the IHC studies comprised 490 invasive ductal carcinoma (IDC) cases and 26 mastopathy tissues. Western blot and RT-qPCR were performed on four breast cancer cell lines (MCF-7, BT-474, SK-BR-3 and MDA-MB-231) and the HME1-hTERT (Me16C) normal immortalized breast epithelial cell line (control). The Kaplan–Meier plotter tool was employed to analyze the predictive value of overall survival of <i>RBMS3</i> expression at the mRNA level. Cytoplasmatic RBMS3 IHC expression was observed in breast cancer cells and stromal cells. The statistical analysis revealed a significantly decreased RBMS3 expression in the cancer specimens when compared with the mastopathy tissues (<i>p</i> < 0.001). An increased expression of RBMS3 was corelated with HER2(+) cancer specimens (<i>p</i> < 0.05) and ER(−) cancer specimens (<i>p</i> < 0.05). In addition, a statistically significant higher expression of RBMS3 was observed in cancer stromal cells in comparison to the control and cancer cells (<i>p</i> < 0.0001). The statistical analysis demonstrated a significantly higher expression of <i>RBMS3</i> mRNA in the SK-BR-3 cell line compared with all other cell lines (<i>p</i> < 0.05). A positive correlation was revealed between the expression of RBMS3, at both the mRNA and protein levels, and longer overall survival. The differences in the expression of RBMS3 in cancer cells (both in vivo and in vitro) and the stroma of breast cancer with regard to the molecular status of the tumor may indicate that RBMS3 could be a potential novel target for the development of personalized methods of treatment. RBMS3 can be an indicator of longer overall survival for potential use in breast cancer diagnostic process.https://www.mdpi.com/1422-0067/24/3/2866RNA-binding protein 3 (RBMS3)carcinogenesiscancer preventiontarget discoverytarget therapyepithelial–mesenchymal transition (EMT) |
| spellingShingle | Tomasz Górnicki Jakub Lambrinow Monika Mrozowska Hanna Romanowicz Beata Smolarz Aleksandra Piotrowska Agnieszka Gomułkiewicz Marzena Podhorska-Okołów Piotr Dzięgiel Jędrzej Grzegrzółka Expression of RBMS3 in Breast Cancer Progression International Journal of Molecular Sciences RNA-binding protein 3 (RBMS3) carcinogenesis cancer prevention target discovery target therapy epithelial–mesenchymal transition (EMT) |
| title | Expression of RBMS3 in Breast Cancer Progression |
| title_full | Expression of RBMS3 in Breast Cancer Progression |
| title_fullStr | Expression of RBMS3 in Breast Cancer Progression |
| title_full_unstemmed | Expression of RBMS3 in Breast Cancer Progression |
| title_short | Expression of RBMS3 in Breast Cancer Progression |
| title_sort | expression of rbms3 in breast cancer progression |
| topic | RNA-binding protein 3 (RBMS3) carcinogenesis cancer prevention target discovery target therapy epithelial–mesenchymal transition (EMT) |
| url | https://www.mdpi.com/1422-0067/24/3/2866 |
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