Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae
In this study, the 16S rRNA of the marine endophytic actinomycete was characterized and identified as Nocardiopsis sp. strain DMS 2 (MH900226). Partial purification and spectroscopies data were interpreted and confirmed as 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane. Anti-bacterial activit...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364720303190 |
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| author | Govindan Nadar Rajivgandhi Govindan Ramachandran C. Chenthis Kanisha Jia-Ling Li Lingzi Yin Natesan Manoharan Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li |
| author_facet | Govindan Nadar Rajivgandhi Govindan Ramachandran C. Chenthis Kanisha Jia-Ling Li Lingzi Yin Natesan Manoharan Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li |
| author_sort | Govindan Nadar Rajivgandhi |
| collection | DOAJ |
| description | In this study, the 16S rRNA of the marine endophytic actinomycete was characterized and identified as Nocardiopsis sp. strain DMS 2 (MH900226). Partial purification and spectroscopies data were interpreted and confirmed as 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane. Anti-bacterial activity of the crude extract and partially purified compound were shown highest inhibition activity against biofilm forming K. pneumoniae. Minimum biofilm inhibition concentration of identified compound against K. pneumoniae was exhibited at the concentration of 300 µg/mL. Decreased metabolic activity and modified exopolysaccharide production of biofilm forming cells were observed at 350 µg/mL and 300 µg/mL concentration. At the same biofilm inhibition concentration, the intracellular architecture of the biofilm cells and extracellular shape modifications were clearly viewed by confocal laser scanning electron microscope and scanning electron microscope respectively. All the in-vitro inhibition results and microscopic images were strongly suggested that the identified compound was a potential anti-biofilm agent against tested K. pneumoniae. |
| first_indexed | 2024-12-12T00:29:05Z |
| format | Article |
| id | doaj.art-f24e5d887cd0422096716e7f08f1321f |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-12T00:29:05Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-f24e5d887cd0422096716e7f08f1321f2022-12-22T00:44:32ZengElsevierJournal of King Saud University: Science1018-36472020-12-0132834953502Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniaeGovindan Nadar Rajivgandhi0Govindan Ramachandran1C. Chenthis Kanisha2Jia-Ling Li3Lingzi Yin4Natesan Manoharan5Naiyf S. Alharbi6Shine Kadaikunnan7Jamal M. Khaled8Wen-Jun Li9State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaNoorul Islam Centre for Higher Education, Thuckalay, Kumaracoil, Tamil Nadu 629180, IndiaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR ChinaDepartment of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, PR China; Corresponding author at: School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.In this study, the 16S rRNA of the marine endophytic actinomycete was characterized and identified as Nocardiopsis sp. strain DMS 2 (MH900226). Partial purification and spectroscopies data were interpreted and confirmed as 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane. Anti-bacterial activity of the crude extract and partially purified compound were shown highest inhibition activity against biofilm forming K. pneumoniae. Minimum biofilm inhibition concentration of identified compound against K. pneumoniae was exhibited at the concentration of 300 µg/mL. Decreased metabolic activity and modified exopolysaccharide production of biofilm forming cells were observed at 350 µg/mL and 300 µg/mL concentration. At the same biofilm inhibition concentration, the intracellular architecture of the biofilm cells and extracellular shape modifications were clearly viewed by confocal laser scanning electron microscope and scanning electron microscope respectively. All the in-vitro inhibition results and microscopic images were strongly suggested that the identified compound was a potential anti-biofilm agent against tested K. pneumoniae.http://www.sciencedirect.com/science/article/pii/S1018364720303190Marine environmentEndophytesNocardiopsisMinimum biofilm inhibition concentrationMetabolic activityIntracellular damages |
| spellingShingle | Govindan Nadar Rajivgandhi Govindan Ramachandran C. Chenthis Kanisha Jia-Ling Li Lingzi Yin Natesan Manoharan Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae Journal of King Saud University: Science Marine environment Endophytes Nocardiopsis Minimum biofilm inhibition concentration Metabolic activity Intracellular damages |
| title | Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae |
| title_full | Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae |
| title_fullStr | Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae |
| title_full_unstemmed | Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae |
| title_short | Anti-biofilm compound of 1, 4-diaza-2, 5-dioxo-3-isobutyl bicyclo[4.3.0]nonane from marine Nocardiopsis sp. DMS 2 (MH900226) against biofilm forming K. pneumoniae |
| title_sort | anti biofilm compound of 1 4 diaza 2 5 dioxo 3 isobutyl bicyclo 4 3 0 nonane from marine nocardiopsis sp dms 2 mh900226 against biofilm forming k pneumoniae |
| topic | Marine environment Endophytes Nocardiopsis Minimum biofilm inhibition concentration Metabolic activity Intracellular damages |
| url | http://www.sciencedirect.com/science/article/pii/S1018364720303190 |
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