Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion
Living sample viability measurement is an extremely common process in medical, pharmaceutical, and biological fields, especially drug pharmacology and toxicology detection. Nowadays, there are a number of chemical, optical, and mechanical methods that have been developed in response to the growing d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/7/453 |
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| author | Hamzah Al-madani Hui Du Junlie Yao Hao Peng Chenyang Yao Bo Jiang Aiguo Wu Fang Yang |
| author_facet | Hamzah Al-madani Hui Du Junlie Yao Hao Peng Chenyang Yao Bo Jiang Aiguo Wu Fang Yang |
| author_sort | Hamzah Al-madani |
| collection | DOAJ |
| description | Living sample viability measurement is an extremely common process in medical, pharmaceutical, and biological fields, especially drug pharmacology and toxicology detection. Nowadays, there are a number of chemical, optical, and mechanical methods that have been developed in response to the growing demand for simple, rapid, accurate, and reliable real-time living sample viability assessment. In parallel, the development trend of viability measurement methods (VMMs) has increasingly shifted from traditional assays towards the innovative atomic force microscope (AFM) oscillating sensor method (referred to as nanomotion), which takes advantage of the adhesion of living samples to an oscillating surface. Herein, we provide a comprehensive review of the common VMMs, laying emphasis on their benefits and drawbacks, as well as evaluating the potential utility of VMMs. In addition, we discuss the nanomotion technique, focusing on its applications, sample attachment protocols, and result display methods. Furthermore, the challenges and future perspectives on nanomotion are commented on, mainly emphasizing scientific restrictions and development orientations. |
| first_indexed | 2024-03-09T10:22:45Z |
| format | Article |
| id | doaj.art-f24fb91af2064bcfbe8702e60d2aae0a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T10:22:45Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-f24fb91af2064bcfbe8702e60d2aae0a2023-12-01T21:56:48ZengMDPI AGBiosensors2079-63742022-06-0112745310.3390/bios12070453Living Sample Viability Measurement Methods from Traditional Assays to NanomotionHamzah Al-madani0Hui Du1Junlie Yao2Hao Peng3Chenyang Yao4Bo Jiang5Aiguo Wu6Fang Yang7Cixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaCixi Institute of Biomedical Engineering, International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS), Key Laboratory of Magnetic Materials and Devices, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, CAS, Ningbo 315201, ChinaLiving sample viability measurement is an extremely common process in medical, pharmaceutical, and biological fields, especially drug pharmacology and toxicology detection. Nowadays, there are a number of chemical, optical, and mechanical methods that have been developed in response to the growing demand for simple, rapid, accurate, and reliable real-time living sample viability assessment. In parallel, the development trend of viability measurement methods (VMMs) has increasingly shifted from traditional assays towards the innovative atomic force microscope (AFM) oscillating sensor method (referred to as nanomotion), which takes advantage of the adhesion of living samples to an oscillating surface. Herein, we provide a comprehensive review of the common VMMs, laying emphasis on their benefits and drawbacks, as well as evaluating the potential utility of VMMs. In addition, we discuss the nanomotion technique, focusing on its applications, sample attachment protocols, and result display methods. Furthermore, the challenges and future perspectives on nanomotion are commented on, mainly emphasizing scientific restrictions and development orientations.https://www.mdpi.com/2079-6374/12/7/453living sample viability measurementatomic force microscopyAFM oscillating sensor methodnanomotion |
| spellingShingle | Hamzah Al-madani Hui Du Junlie Yao Hao Peng Chenyang Yao Bo Jiang Aiguo Wu Fang Yang Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion Biosensors living sample viability measurement atomic force microscopy AFM oscillating sensor method nanomotion |
| title | Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion |
| title_full | Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion |
| title_fullStr | Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion |
| title_full_unstemmed | Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion |
| title_short | Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion |
| title_sort | living sample viability measurement methods from traditional assays to nanomotion |
| topic | living sample viability measurement atomic force microscopy AFM oscillating sensor method nanomotion |
| url | https://www.mdpi.com/2079-6374/12/7/453 |
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