Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards
Regulatory Standards and Forensic Communities are expressing an expectation for HID products to be certified as “DNA-free.” Recently, “DNA-free” status was described for HID-related products using ethylene oxide (EtO); this gas reduces the presence of amplifiable DNA and causes minimal interference...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Forensic Science and Medicine |
| Subjects: | |
| Online Access: | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=2;spage=67;epage=73;aulast=Moran |
| _version_ | 1819208603312062464 |
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| author | Nina Moran Peter James Tatnell |
| author_facet | Nina Moran Peter James Tatnell |
| author_sort | Nina Moran |
| collection | DOAJ |
| description | Regulatory Standards and Forensic Communities are expressing an expectation for HID products to be certified as “DNA-free.” Recently, “DNA-free” status was described for HID-related products using ethylene oxide (EtO); this gas reduces the presence of amplifiable DNA and causes minimal interference to downstream HID-analytical methods. During sample collection, indicating cards, for example, Indicating FTA™ (GE Healthcare Life Sciences, UK), are used to collect and store buccal cell DNA. These cards contain a dye which changes color on application of a colorless sample. Generating “DNA-free” indicating cards using EtO should not impact the dyes' ability to indicate sample location or the efficacy of the card in downstream HID-analytical methods. This study was initiated to identify alternative dyes to those currently used with sample indicating collection cards. The most promising, dyes when applied to cellulose papers exhibited a uniform color distribution and excellent sample indicating properties even when mixed with chemicals associated with FTA™. When dyed cellulose papers were exposed to EtO, ultraviolet radiation, elevated temperature, and humidity, negligible fading or discoloration was observed. The presence of these dyes on cellulose papers did not interfere with direct short tandem repeat (STR) profiling. Allelic concordance, first pass success rate, and mean peak heights were comparable to samples applied to Indicating FTA. Biological samples applied to EtO-treated dyed cellulose papers and stored >1 month produced full STR profiles of sufficient quality to allow submission to DNA databases, confirming negligible interference from EtO treatment. These alternative sample indicating dyes resist EtO-mediated fading while fulfilling the Forensic Community's expectation for “DNA-free” with negligible impact on collection card performance. |
| first_indexed | 2024-12-23T05:42:01Z |
| format | Article |
| id | doaj.art-f2506a5bd2d44cf9a11ea00cb95ba0dc |
| institution | Directory Open Access Journal |
| issn | 2349-5014 |
| language | English |
| last_indexed | 2024-12-23T05:42:01Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Forensic Science and Medicine |
| spelling | doaj.art-f2506a5bd2d44cf9a11ea00cb95ba0dc2022-12-21T17:58:10ZengWolters Kluwer Medknow PublicationsJournal of Forensic Science and Medicine2349-50142016-01-0122677310.4103/2349-5014.184191Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection CardsNina MoranPeter James TatnellRegulatory Standards and Forensic Communities are expressing an expectation for HID products to be certified as “DNA-free.” Recently, “DNA-free” status was described for HID-related products using ethylene oxide (EtO); this gas reduces the presence of amplifiable DNA and causes minimal interference to downstream HID-analytical methods. During sample collection, indicating cards, for example, Indicating FTA™ (GE Healthcare Life Sciences, UK), are used to collect and store buccal cell DNA. These cards contain a dye which changes color on application of a colorless sample. Generating “DNA-free” indicating cards using EtO should not impact the dyes' ability to indicate sample location or the efficacy of the card in downstream HID-analytical methods. This study was initiated to identify alternative dyes to those currently used with sample indicating collection cards. The most promising, dyes when applied to cellulose papers exhibited a uniform color distribution and excellent sample indicating properties even when mixed with chemicals associated with FTA™. When dyed cellulose papers were exposed to EtO, ultraviolet radiation, elevated temperature, and humidity, negligible fading or discoloration was observed. The presence of these dyes on cellulose papers did not interfere with direct short tandem repeat (STR) profiling. Allelic concordance, first pass success rate, and mean peak heights were comparable to samples applied to Indicating FTA. Biological samples applied to EtO-treated dyed cellulose papers and stored >1 month produced full STR profiles of sufficient quality to allow submission to DNA databases, confirming negligible interference from EtO treatment. These alternative sample indicating dyes resist EtO-mediated fading while fulfilling the Forensic Community's expectation for “DNA-free” with negligible impact on collection card performance.http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=2;spage=67;epage=73;aulast=MoranEthylene oxideforensic DNA analysisidentification of colored dyesIndicating FTA sample collection cardsshort tandem repeat profiling |
| spellingShingle | Nina Moran Peter James Tatnell Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards Journal of Forensic Science and Medicine Ethylene oxide forensic DNA analysis identification of colored dyes Indicating FTA sample collection cards short tandem repeat profiling |
| title | Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards |
| title_full | Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards |
| title_fullStr | Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards |
| title_full_unstemmed | Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards |
| title_short | Identification of Colored Dyes that are Resistant to Fading on Exposure to Ethylene Oxide; Use with Indicating FTA™ Sample Collection Cards |
| title_sort | identification of colored dyes that are resistant to fading on exposure to ethylene oxide use with indicating fta™ sample collection cards |
| topic | Ethylene oxide forensic DNA analysis identification of colored dyes Indicating FTA sample collection cards short tandem repeat profiling |
| url | http://www.jfsmonline.com/article.asp?issn=2349-5014;year=2016;volume=2;issue=2;spage=67;epage=73;aulast=Moran |
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