Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections
In this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like geno...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2021-01-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200159/parasite200159.html |
| _version_ | 1797427608414060544 |
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| author | Rodriguez-Anaya Libia Zulema Félix-Sastré Ángel Josué Lares-Villa Fernando Lares-Jiménez Luis Fernando Gonzalez-Galaviz Jose Reyes |
| author_facet | Rodriguez-Anaya Libia Zulema Félix-Sastré Ángel Josué Lares-Villa Fernando Lares-Jiménez Luis Fernando Gonzalez-Galaviz Jose Reyes |
| author_sort | Rodriguez-Anaya Libia Zulema |
| collection | DOAJ |
| description | In this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like genomics, transcriptomics, and proteomics. Currently, novel data produced through comparative genomics analyses and both differential gene and protein expression in these free-living amoebas have allowed for breakthroughs to identify genes unique to N. fowleri, genes with active transcriptional activity, and their differential expression in conditions of modified virulence. Furthermore, orthologous genes of the various nuclear genomes within the Naegleria and Acanthamoeba genera have been clustered. The proteome of B. mandrillaris has been reconstructed through transcriptome data, and its mitochondrial genome structure has been thoroughly described with a unique characteristic that has come to light: a type I intron with the capacity of interrupting genes through its self-splicing ribozymes activity. With the integration of data derived from the diverse omic sciences, there is a potential approximation that reflects the molecular complexity required for the identification of virulence factors, as well as crucial information regarding the comprehension of the molecular mechanisms with which these interact. Altogether, these breakthroughs could contribute to radical advances in both the fields of therapy design and medical diagnosis in the foreseeable future. |
| first_indexed | 2024-03-09T08:46:21Z |
| format | Article |
| id | doaj.art-f2508d54879d4dd4854cc482016c318e |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:46:21Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-f2508d54879d4dd4854cc482016c318e2023-12-02T15:22:36ZengEDP SciencesParasite1776-10422021-01-01283610.1051/parasite/2021033parasite200159Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projectionsRodriguez-Anaya Libia Zulema0https://orcid.org/0000-0003-1643-3938Félix-Sastré Ángel Josué1https://orcid.org/0000-0001-8147-2103Lares-Villa Fernando2https://orcid.org/0000-0002-2407-9409Lares-Jiménez Luis Fernando3Gonzalez-Galaviz Jose Reyes4https://orcid.org/0000-0001-7235-9328CONACYT-Instituto Tecnológico de SonoraDepartamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de SonoraDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de SonoraDepartamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de SonoraCONACYT-Instituto Tecnológico de SonoraIn this review, we focus on the sequenced genomes of the pathogens Naegleria fowleri, Acanthamoeba spp. and Balamuthia mandrillaris, and the remarkable discoveries regarding the pathogenicity and genetic information of these organisms, using techniques related to the various omics branches like genomics, transcriptomics, and proteomics. Currently, novel data produced through comparative genomics analyses and both differential gene and protein expression in these free-living amoebas have allowed for breakthroughs to identify genes unique to N. fowleri, genes with active transcriptional activity, and their differential expression in conditions of modified virulence. Furthermore, orthologous genes of the various nuclear genomes within the Naegleria and Acanthamoeba genera have been clustered. The proteome of B. mandrillaris has been reconstructed through transcriptome data, and its mitochondrial genome structure has been thoroughly described with a unique characteristic that has come to light: a type I intron with the capacity of interrupting genes through its self-splicing ribozymes activity. With the integration of data derived from the diverse omic sciences, there is a potential approximation that reflects the molecular complexity required for the identification of virulence factors, as well as crucial information regarding the comprehension of the molecular mechanisms with which these interact. Altogether, these breakthroughs could contribute to radical advances in both the fields of therapy design and medical diagnosis in the foreseeable future.https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200159/parasite200159.htmlfree-living amoebasnext generation sequencinggenomictranscriptomicproteomicomics |
| spellingShingle | Rodriguez-Anaya Libia Zulema Félix-Sastré Ángel Josué Lares-Villa Fernando Lares-Jiménez Luis Fernando Gonzalez-Galaviz Jose Reyes Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections Parasite free-living amoebas next generation sequencing genomic transcriptomic proteomic omics |
| title | Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections |
| title_full | Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections |
| title_fullStr | Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections |
| title_full_unstemmed | Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections |
| title_short | Application of the omics sciences to the study of Naegleria fowleri, Acanthamoeba spp., and Balamuthia mandrillaris: current status and future projections |
| title_sort | application of the omics sciences to the study of naegleria fowleri acanthamoeba spp and balamuthia mandrillaris current status and future projections |
| topic | free-living amoebas next generation sequencing genomic transcriptomic proteomic omics |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite200159/parasite200159.html |
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