Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus

The leaf is the main organ for rapeseed photosynthesis, and its morphology influences photosynthetic efficiency and supports increased planting density and yield. However, the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood, restricting progress in breeding f...

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Main Authors: Yingchao Tan, Lanyang Ren, Jia Wang, Shuyao Ran, Liusha Wu, Ziyi Cheng, Cunmin Qu, Jiana Li, Liezhao Liu
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2023-06-01
Series:Crop Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2214514122002501
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author Yingchao Tan
Lanyang Ren
Jia Wang
Shuyao Ran
Liusha Wu
Ziyi Cheng
Cunmin Qu
Jiana Li
Liezhao Liu
author_facet Yingchao Tan
Lanyang Ren
Jia Wang
Shuyao Ran
Liusha Wu
Ziyi Cheng
Cunmin Qu
Jiana Li
Liezhao Liu
author_sort Yingchao Tan
collection DOAJ
description The leaf is the main organ for rapeseed photosynthesis, and its morphology influences photosynthetic efficiency and supports increased planting density and yield. However, the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood, restricting progress in breeding for the trait. We describe a novel dominant mutation, curly leaf 1 (cl1), which confers uneven dorsal–ventral axis development, irregular cellular structure and influenced gravitropic response in the seedling stage. The CL1 locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat (SSR) markers, and co-segregated with the phenotype of plants in the curly F2 population. A substitution (P62S) was identified in the highly conserved degron motif (GWSPV) of the IAA2 protein in the cl1 mutant, and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin, influencing auxin signaling. The P62S substitution-induced curly leaf phenotype was verified by ectopic expression of BnaA05.iaa2 in Arabidopsis and B. napus. Our findings explain the function of IAA2 in rapeseed, providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development in B. napus.
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spelling doaj.art-f255f4f15d5c4bc895b6be9bf2ec36632023-06-10T04:27:30ZengKeAi Communications Co., Ltd.Crop Journal2214-51412023-06-01113756765Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napusYingchao Tan0Lanyang Ren1Jia Wang2Shuyao Ran3Liusha Wu4Ziyi Cheng5Cunmin Qu6Jiana Li7Liezhao Liu8College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Rapeseed, Academy of Agricultural Sciences, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Southwest University, Chongqing 400715, China; Corresponding author.The leaf is the main organ for rapeseed photosynthesis, and its morphology influences photosynthetic efficiency and supports increased planting density and yield. However, the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood, restricting progress in breeding for the trait. We describe a novel dominant mutation, curly leaf 1 (cl1), which confers uneven dorsal–ventral axis development, irregular cellular structure and influenced gravitropic response in the seedling stage. The CL1 locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat (SSR) markers, and co-segregated with the phenotype of plants in the curly F2 population. A substitution (P62S) was identified in the highly conserved degron motif (GWSPV) of the IAA2 protein in the cl1 mutant, and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin, influencing auxin signaling. The P62S substitution-induced curly leaf phenotype was verified by ectopic expression of BnaA05.iaa2 in Arabidopsis and B. napus. Our findings explain the function of IAA2 in rapeseed, providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development in B. napus.http://www.sciencedirect.com/science/article/pii/S2214514122002501Brassica napusCurly leafAuxinIAA2 protein
spellingShingle Yingchao Tan
Lanyang Ren
Jia Wang
Shuyao Ran
Liusha Wu
Ziyi Cheng
Cunmin Qu
Jiana Li
Liezhao Liu
Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
Crop Journal
Brassica napus
Curly leaf
Auxin
IAA2 protein
title Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
title_full Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
title_fullStr Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
title_full_unstemmed Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
title_short Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus
title_sort identification and characterization of a curly leaf locus cl1 encoding an iaa2 protein in brassica napus
topic Brassica napus
Curly leaf
Auxin
IAA2 protein
url http://www.sciencedirect.com/science/article/pii/S2214514122002501
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