Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway
Abstract Background Tumor metastasis is responsible for the high mortality rate of patients with oral squamous cell carcinoma (OSCC). Although many hypotheses have been proposed to elucidate the mechanism of tumor metastasis, the origin of the metastatic tumor cells remains unclear. In this study, w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.6940 |
| _version_ | 1827320474777944064 |
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| author | Chengwan Xia Qian Zhang Yumei Pu Qingang Hu Yuxin Wang |
| author_facet | Chengwan Xia Qian Zhang Yumei Pu Qingang Hu Yuxin Wang |
| author_sort | Chengwan Xia |
| collection | DOAJ |
| description | Abstract Background Tumor metastasis is responsible for the high mortality rate of patients with oral squamous cell carcinoma (OSCC). Although many hypotheses have been proposed to elucidate the mechanism of tumor metastasis, the origin of the metastatic tumor cells remains unclear. In this study, we explored the role of cell fusion in the formation of OSCC metastatic tumor cells. Methods Murine OSCC tumor cells and macrophages were fused in vitro, and the cell proliferation, migration, and phagocytosis abilities of hybrid cells and parental cells were compared. Subsequently, we compared the transcriptome differences between hybrid and parental cells. Results Murine OSCC tumor cells and macrophages were successfully fused in vitro. The cytological and molecular experimental results revealed that OSCC tumor cells obtained a migration‐related phenotype after fusion with macrophages, and the migration ability of hybrid cells was related to the activation of the “chemokine signal pathway”. Conclusion After fusion with macrophages, the chemokine signaling pathway in OSCC tumor cells was activated, leading to metastasis. |
| first_indexed | 2024-04-25T00:45:46Z |
| format | Article |
| id | doaj.art-f2566bf0177c47118be7857c9b543678 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-25T00:45:46Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-f2566bf0177c47118be7857c9b5436782024-03-12T04:52:34ZengWileyCancer Medicine2045-76342024-02-01134n/an/a10.1002/cam4.6940Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathwayChengwan Xia0Qian Zhang1Yumei Pu2Qingang Hu3Yuxin Wang4Department of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing ChinaDepartment of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing ChinaDepartment of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing ChinaDepartment of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing ChinaDepartment of Oral and Maxillofacial Trauma Orthognathic Plastic Surgery Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing ChinaAbstract Background Tumor metastasis is responsible for the high mortality rate of patients with oral squamous cell carcinoma (OSCC). Although many hypotheses have been proposed to elucidate the mechanism of tumor metastasis, the origin of the metastatic tumor cells remains unclear. In this study, we explored the role of cell fusion in the formation of OSCC metastatic tumor cells. Methods Murine OSCC tumor cells and macrophages were fused in vitro, and the cell proliferation, migration, and phagocytosis abilities of hybrid cells and parental cells were compared. Subsequently, we compared the transcriptome differences between hybrid and parental cells. Results Murine OSCC tumor cells and macrophages were successfully fused in vitro. The cytological and molecular experimental results revealed that OSCC tumor cells obtained a migration‐related phenotype after fusion with macrophages, and the migration ability of hybrid cells was related to the activation of the “chemokine signal pathway”. Conclusion After fusion with macrophages, the chemokine signaling pathway in OSCC tumor cells was activated, leading to metastasis.https://doi.org/10.1002/cam4.6940cell fusionchemokine signaling pathwaymacrophagesOSCCtumor metastasis |
| spellingShingle | Chengwan Xia Qian Zhang Yumei Pu Qingang Hu Yuxin Wang Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway Cancer Medicine cell fusion chemokine signaling pathway macrophages OSCC tumor metastasis |
| title | Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway |
| title_full | Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway |
| title_fullStr | Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway |
| title_full_unstemmed | Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway |
| title_short | Cell fusion between tumor cells and macrophages promotes the metastasis of OSCC patient through the activation of the chemokine signaling pathway |
| title_sort | cell fusion between tumor cells and macrophages promotes the metastasis of oscc patient through the activation of the chemokine signaling pathway |
| topic | cell fusion chemokine signaling pathway macrophages OSCC tumor metastasis |
| url | https://doi.org/10.1002/cam4.6940 |
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