Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells
In mesophyll cells of the aquatic monocot <i>Vallisneria</i>, red light induces rotational cytoplasmic streaming, which is regulated by the cytoplasmic concentration of Ca<sup>2+</sup>. Our previous investigations revealed that red light induces Ca<sup>2+</sup> ef...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/3/376 |
| _version_ | 1798019255912890368 |
|---|---|
| author | Akiko Harada Yoshiji Okazaki Toshinori Kinoshita Reiko Nagai Shingo Takagi |
| author_facet | Akiko Harada Yoshiji Okazaki Toshinori Kinoshita Reiko Nagai Shingo Takagi |
| author_sort | Akiko Harada |
| collection | DOAJ |
| description | In mesophyll cells of the aquatic monocot <i>Vallisneria</i>, red light induces rotational cytoplasmic streaming, which is regulated by the cytoplasmic concentration of Ca<sup>2+</sup>. Our previous investigations revealed that red light induces Ca<sup>2+</sup> efflux across the plasma membrane (PM), and that both the red light-induced cytoplasmic streaming and the Ca<sup>2+</sup> efflux are sensitive to vanadate, an inhibitor of P-type ATPases. In this study, pharmacological experiments suggested the involvement of PM H<sup>+</sup>-ATPase, one of the P-type ATPases, in the photoinduction of cytoplasmic streaming. We hypothesized that red light would activate PM H<sup>+</sup>-ATPase to generate a large H<sup>+</sup> motive force (PMF) in a photosynthesis-dependent manner. We demonstrated that indeed, photosynthesis increased the PMF and induced phosphorylation of the penultimate residue, threonine, of PM H<sup>+</sup>-ATPase, which is a major activation mechanism of H<sup>+</sup>-ATPase. The results suggested that a large PMF generated by PM H<sup>+</sup>-ATPase energizes the Ca<sup>2+</sup> efflux across the PM. As expected, we detected a putative Ca<sup>2+</sup>/H<sup>+</sup> exchange activity in PM vesicles isolated from <i>Vallisneria</i> leaves. |
| first_indexed | 2024-04-11T16:37:43Z |
| format | Article |
| id | doaj.art-f25bf244855149ea9c71e7ae036a62d7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-04-11T16:37:43Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f25bf244855149ea9c71e7ae036a62d72022-12-22T04:13:48ZengMDPI AGPlants2223-77472020-03-019337610.3390/plants9030376plants9030376Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll CellsAkiko Harada0Yoshiji Okazaki1Toshinori Kinoshita2Reiko Nagai3Shingo Takagi4Department of Biology, Osaka Medical College, Takatsuki, Osaka 569-8686, JapanDepartment of Biology, Osaka Medical College, Takatsuki, Osaka 569-8686, JapanDivision of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, JapanDepartment of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, JapanDepartment of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, JapanIn mesophyll cells of the aquatic monocot <i>Vallisneria</i>, red light induces rotational cytoplasmic streaming, which is regulated by the cytoplasmic concentration of Ca<sup>2+</sup>. Our previous investigations revealed that red light induces Ca<sup>2+</sup> efflux across the plasma membrane (PM), and that both the red light-induced cytoplasmic streaming and the Ca<sup>2+</sup> efflux are sensitive to vanadate, an inhibitor of P-type ATPases. In this study, pharmacological experiments suggested the involvement of PM H<sup>+</sup>-ATPase, one of the P-type ATPases, in the photoinduction of cytoplasmic streaming. We hypothesized that red light would activate PM H<sup>+</sup>-ATPase to generate a large H<sup>+</sup> motive force (PMF) in a photosynthesis-dependent manner. We demonstrated that indeed, photosynthesis increased the PMF and induced phosphorylation of the penultimate residue, threonine, of PM H<sup>+</sup>-ATPase, which is a major activation mechanism of H<sup>+</sup>-ATPase. The results suggested that a large PMF generated by PM H<sup>+</sup>-ATPase energizes the Ca<sup>2+</sup> efflux across the PM. As expected, we detected a putative Ca<sup>2+</sup>/H<sup>+</sup> exchange activity in PM vesicles isolated from <i>Vallisneria</i> leaves.https://www.mdpi.com/2223-7747/9/3/376h<sup>+</sup> motive forceplasma membrane h<sup>+</sup>-atpasephotosynthesiscytoplasmic streamingca<sup>2+</sup><i>vallisneria</i> |
| spellingShingle | Akiko Harada Yoshiji Okazaki Toshinori Kinoshita Reiko Nagai Shingo Takagi Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells Plants h<sup>+</sup> motive force plasma membrane h<sup>+</sup>-atpase photosynthesis cytoplasmic streaming ca<sup>2+</sup> <i>vallisneria</i> |
| title | Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells |
| title_full | Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells |
| title_fullStr | Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells |
| title_full_unstemmed | Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells |
| title_short | Role of Proton Motive Force in Photoinduction of Cytoplasmic Streaming in <i>Vallisneria</i> Mesophyll Cells |
| title_sort | role of proton motive force in photoinduction of cytoplasmic streaming in i vallisneria i mesophyll cells |
| topic | h<sup>+</sup> motive force plasma membrane h<sup>+</sup>-atpase photosynthesis cytoplasmic streaming ca<sup>2+</sup> <i>vallisneria</i> |
| url | https://www.mdpi.com/2223-7747/9/3/376 |
| work_keys_str_mv | AT akikoharada roleofprotonmotiveforceinphotoinductionofcytoplasmicstreaminginivallisneriaimesophyllcells AT yoshijiokazaki roleofprotonmotiveforceinphotoinductionofcytoplasmicstreaminginivallisneriaimesophyllcells AT toshinorikinoshita roleofprotonmotiveforceinphotoinductionofcytoplasmicstreaminginivallisneriaimesophyllcells AT reikonagai roleofprotonmotiveforceinphotoinductionofcytoplasmicstreaminginivallisneriaimesophyllcells AT shingotakagi roleofprotonmotiveforceinphotoinductionofcytoplasmicstreaminginivallisneriaimesophyllcells |