Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury
Retinal ischaemia-reperfusion (IR) injury is implicated in many sight-threatening diseases. The reperfusion of tissue induces oxidative stress and cell damage. Sulforaphane, which is abundant in cruciferous vegetables, exerts anti-oxidative effects. We investigated the protective effect of sulforaph...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620302474 |
| _version_ | 1818948803065020416 |
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| author | Hao-Yun Chang Chao-Wen Lin Chung-May Yang Chang-Hao Yang |
| author_facet | Hao-Yun Chang Chao-Wen Lin Chung-May Yang Chang-Hao Yang |
| author_sort | Hao-Yun Chang |
| collection | DOAJ |
| description | Retinal ischaemia-reperfusion (IR) injury is implicated in many sight-threatening diseases. The reperfusion of tissue induces oxidative stress and cell damage. Sulforaphane, which is abundant in cruciferous vegetables, exerts anti-oxidative effects. We investigated the protective effect of sulforaphane against oxidative stress or IR injury in retinal cells. We utilized ARPE-19 cells and used tert-butyl hydroperoxide to induce oxidative stress. Cell survival improved after sulforaphane treatment. Sulforaphane attenuated reactive oxygen species production and diminished mitochondrial dysfunction. Furthermore, sulforaphane activated phase II detoxification enzymes and suppressed pro-inflammatory mediators. We transiently raised the intraocular pressure of rats to establish a model of retinal IR injury. High-dose sulforaphane pretreatment ameliorated b-wave decrement in electroretinogram, indicating that sulforaphane could preserve retinal function. Electrophoretic mobility shift assay revealed that these effects originated from upregulation of Nrf2 and downregulation of NF-kB pathway. Our results demonstrated that sulforaphane has a dose-dependent protective effect against oxidative stress and IR injury. |
| first_indexed | 2024-12-20T08:52:36Z |
| format | Article |
| id | doaj.art-f25e329e83004004b422f8e3bb3551a5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T08:52:36Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f25e329e83004004b422f8e3bb3551a52022-12-21T19:46:06ZengElsevierJournal of Functional Foods1756-46462020-08-0171104023Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injuryHao-Yun Chang0Chao-Wen Lin1Chung-May Yang2Chang-Hao Yang3Department of Internal Medicine, National Taiwan University Hospital, No. 7, Zhongshan South Road, Taipei 100, TaiwanDepartments of Ophthalmology, National Taiwan University Hospital, No. 7, Zhongshan South Road, Taipei 100, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, No. 1, Jen Ai Road Section 1, Taipei 100, TaiwanDepartments of Ophthalmology, National Taiwan University Hospital, No. 7, Zhongshan South Road, Taipei 100, Taiwan; College of Medicine, National Taiwan University, No. 1, Jen Ai Road Section 1, Taipei 100, TaiwanDepartments of Ophthalmology, National Taiwan University Hospital, No. 7, Zhongshan South Road, Taipei 100, Taiwan; College of Medicine, National Taiwan University, No. 1, Jen Ai Road Section 1, Taipei 100, Taiwan; Corresponding author at: Departments of Ophthalmology, National Taiwan University Hospital, No. 7, Zhongshan South Road, Taipei 100, Taiwan.Retinal ischaemia-reperfusion (IR) injury is implicated in many sight-threatening diseases. The reperfusion of tissue induces oxidative stress and cell damage. Sulforaphane, which is abundant in cruciferous vegetables, exerts anti-oxidative effects. We investigated the protective effect of sulforaphane against oxidative stress or IR injury in retinal cells. We utilized ARPE-19 cells and used tert-butyl hydroperoxide to induce oxidative stress. Cell survival improved after sulforaphane treatment. Sulforaphane attenuated reactive oxygen species production and diminished mitochondrial dysfunction. Furthermore, sulforaphane activated phase II detoxification enzymes and suppressed pro-inflammatory mediators. We transiently raised the intraocular pressure of rats to establish a model of retinal IR injury. High-dose sulforaphane pretreatment ameliorated b-wave decrement in electroretinogram, indicating that sulforaphane could preserve retinal function. Electrophoretic mobility shift assay revealed that these effects originated from upregulation of Nrf2 and downregulation of NF-kB pathway. Our results demonstrated that sulforaphane has a dose-dependent protective effect against oxidative stress and IR injury.http://www.sciencedirect.com/science/article/pii/S1756464620302474Oxidative stressSulforaphaneIschaemia-reperfusion injuryNrf-2NF-κB |
| spellingShingle | Hao-Yun Chang Chao-Wen Lin Chung-May Yang Chang-Hao Yang Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury Journal of Functional Foods Oxidative stress Sulforaphane Ischaemia-reperfusion injury Nrf-2 NF-κB |
| title | Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury |
| title_full | Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury |
| title_fullStr | Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury |
| title_full_unstemmed | Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury |
| title_short | Nrf-2 activator sulforaphane protects retinal cells from oxidative stress-induced retinal injury |
| title_sort | nrf 2 activator sulforaphane protects retinal cells from oxidative stress induced retinal injury |
| topic | Oxidative stress Sulforaphane Ischaemia-reperfusion injury Nrf-2 NF-κB |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620302474 |
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