Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.
The angiomotin (Amot)-Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remai...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-05-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.3000253 |
| _version_ | 1798033604397236224 |
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| author | Katarzyna O Rojek Joanna Krzemień Hubert Doleżyczek Paweł M Boguszewski Leszek Kaczmarek Witold Konopka Marcin Rylski Jacek Jaworski Lars Holmgren Tomasz J Prószyński |
| author_facet | Katarzyna O Rojek Joanna Krzemień Hubert Doleżyczek Paweł M Boguszewski Leszek Kaczmarek Witold Konopka Marcin Rylski Jacek Jaworski Lars Holmgren Tomasz J Prószyński |
| author_sort | Katarzyna O Rojek |
| collection | DOAJ |
| description | The angiomotin (Amot)-Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remains unclear. We found that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. The conditional deletion of Amot and Yap1 in neurons led to a decrease in the complexity of Purkinje cell dendritic trees, abnormal cerebellar morphology, and impairments in motor coordination. Our results indicate that the function of Amot and Yap1 in dendrite growth does not rely on interactions with TEA domain (TEAD) transcription factors or the expression of Hippo pathway-dependent genes. Instead, Amot and Yap1 regulate dendrite development by affecting the phosphorylation of S6 kinase and its target S6 ribosomal protein. |
| first_indexed | 2024-04-11T20:32:57Z |
| format | Article |
| id | doaj.art-f25e78cb23d540f684a9070631dea244 |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-04-11T20:32:57Z |
| publishDate | 2019-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-f25e78cb23d540f684a9070631dea2442022-12-22T04:04:27ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852019-05-01175e300025310.1371/journal.pbio.3000253Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice.Katarzyna O RojekJoanna KrzemieńHubert DoleżyczekPaweł M BoguszewskiLeszek KaczmarekWitold KonopkaMarcin RylskiJacek JaworskiLars HolmgrenTomasz J PrószyńskiThe angiomotin (Amot)-Yes-associated protein 1 (Yap1) complex plays a major role in regulating the inhibition of cell contact, cellular polarity, and cell growth in many cell types. However, the function of Amot and the Hippo pathway transcription coactivator Yap1 in the central nervous system remains unclear. We found that Amot is a critical mediator of dendritic morphogenesis in cultured hippocampal cells and Purkinje cells in the brain. Amot function in developing neurons depends on interactions with Yap1, which is also indispensable for dendrite growth and arborization in vitro. The conditional deletion of Amot and Yap1 in neurons led to a decrease in the complexity of Purkinje cell dendritic trees, abnormal cerebellar morphology, and impairments in motor coordination. Our results indicate that the function of Amot and Yap1 in dendrite growth does not rely on interactions with TEA domain (TEAD) transcription factors or the expression of Hippo pathway-dependent genes. Instead, Amot and Yap1 regulate dendrite development by affecting the phosphorylation of S6 kinase and its target S6 ribosomal protein.https://doi.org/10.1371/journal.pbio.3000253 |
| spellingShingle | Katarzyna O Rojek Joanna Krzemień Hubert Doleżyczek Paweł M Boguszewski Leszek Kaczmarek Witold Konopka Marcin Rylski Jacek Jaworski Lars Holmgren Tomasz J Prószyński Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. PLoS Biology |
| title | Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. |
| title_full | Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. |
| title_fullStr | Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. |
| title_full_unstemmed | Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. |
| title_short | Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. |
| title_sort | amot and yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice |
| url | https://doi.org/10.1371/journal.pbio.3000253 |
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