Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones
Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2021-12-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X21000703 |
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| author | Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou |
| author_facet | Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou |
| author_sort | Zhitang Lyu |
| collection | DOAJ |
| description | Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases. Alpha diversity analysis showed that the microbiota richness was similar in bile and intestinal fluid; Beta diversity analysis showed that there were differences in the composition between biliary microbiota and the duodenal microbiota, but the abundance of the main groups showed similarities. The composition of the biliary microbiota from gallstone patients was more complex, as was the duodenal microbiota. Proteobacteria and Firmicutes were the dominant bacteria at phylum level, accounting for at least 75% of the total reads in each subgroup. Pseudomonas and Escherichia−Shigella were the major genus among subgroups, but Escherichia-Shigella had increased abundance in duodenal microbiota with primary choledocholithiasis, which may play an important role in stone formation. It is noteworthy that Clostridiumsensu_stricto, Lachnospiraceae _UCG-008, Butyrivibrio and Roseburia which could produce short chain fatty acids (SCFAs), were significantly decreased in biliary microbiota with primary CBD stones (p < 0.05). Our study provided new insights into the compositional of normal biliary microbiota. The micro-ecology of biliary and duodenal in patients with stones is complex and closely related, and there is a potential for dysbacteriosis. The decrease in abundance of certain major acid-producing bacteria affects the health of the biliary tract and thus leads to the formation of stones. |
| first_indexed | 2024-04-24T08:34:03Z |
| format | Article |
| id | doaj.art-f26084fc5ae74b7fbee5bf7279d275eb |
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| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:34:03Z |
| publishDate | 2021-12-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-f26084fc5ae74b7fbee5bf7279d275eb2024-04-16T18:15:30ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2021-12-0164414428Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stonesZhitang Lyu0Tingting Yu1Lichao Zhang2Xiaona Xu3Yijun Zhang4Jihong Li5Zhirong Li6Wei Zhang7Senlin Hou8School of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding, PR China; Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, PR China; Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Baoding, PR China; Corresponding author. School of Life Sciences, Hebei University, Baoding, 071002, PR China.The Second Hospital of Hebei Medical University, Shijiazhuang, PR ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, PR ChinaSchool of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding, PR China; Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, PR ChinaSchool of Life Sciences, Institute of Life Science and Green Development, Hebei University, Baoding, PR China; Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding, PR ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, PR ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, PR ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, PR ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, PR China; Corresponding author. The Second Hospital of Hebei Medical University, Shijiazhuang, PR China.Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases. Alpha diversity analysis showed that the microbiota richness was similar in bile and intestinal fluid; Beta diversity analysis showed that there were differences in the composition between biliary microbiota and the duodenal microbiota, but the abundance of the main groups showed similarities. The composition of the biliary microbiota from gallstone patients was more complex, as was the duodenal microbiota. Proteobacteria and Firmicutes were the dominant bacteria at phylum level, accounting for at least 75% of the total reads in each subgroup. Pseudomonas and Escherichia−Shigella were the major genus among subgroups, but Escherichia-Shigella had increased abundance in duodenal microbiota with primary choledocholithiasis, which may play an important role in stone formation. It is noteworthy that Clostridiumsensu_stricto, Lachnospiraceae _UCG-008, Butyrivibrio and Roseburia which could produce short chain fatty acids (SCFAs), were significantly decreased in biliary microbiota with primary CBD stones (p < 0.05). Our study provided new insights into the compositional of normal biliary microbiota. The micro-ecology of biliary and duodenal in patients with stones is complex and closely related, and there is a potential for dysbacteriosis. The decrease in abundance of certain major acid-producing bacteria affects the health of the biliary tract and thus leads to the formation of stones.http://www.sciencedirect.com/science/article/pii/S2405805X21000703Primary common bile duct stonesERCPDysbacteriosisBile duct microbiotaDuodenal microbiota16S rRNA gene high-throughput sequencing |
| spellingShingle | Zhitang Lyu Tingting Yu Lichao Zhang Xiaona Xu Yijun Zhang Jihong Li Zhirong Li Wei Zhang Senlin Hou Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones Synthetic and Systems Biotechnology Primary common bile duct stones ERCP Dysbacteriosis Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing |
| title | Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| title_full | Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| title_fullStr | Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| title_full_unstemmed | Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| title_short | Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| title_sort | analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones |
| topic | Primary common bile duct stones ERCP Dysbacteriosis Bile duct microbiota Duodenal microbiota 16S rRNA gene high-throughput sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X21000703 |
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