Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum
Abstract The so-called EPT taxa have been shown to be highly sensitive to various environmental pollutants. However, there are only few published studies on toxicity testing with EPT representatives and there is a particular lack of protocols for chronic toxicity testing, e.g., for integration into...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-11-01
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| Series: | Environmental Sciences Europe |
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| Online Access: | https://doi.org/10.1186/s12302-023-00806-4 |
| _version_ | 1797577546330537984 |
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| author | Kirsten Germing Steve U. Ayobahan Hannes Reinwald Maria Vogt Benedikt Ringbeck Bernd Göckener Elke Eilebrecht Lena Kosak Sebastian Eilebrecht |
| author_facet | Kirsten Germing Steve U. Ayobahan Hannes Reinwald Maria Vogt Benedikt Ringbeck Bernd Göckener Elke Eilebrecht Lena Kosak Sebastian Eilebrecht |
| author_sort | Kirsten Germing |
| collection | DOAJ |
| description | Abstract The so-called EPT taxa have been shown to be highly sensitive to various environmental pollutants. However, there are only few published studies on toxicity testing with EPT representatives and there is a particular lack of protocols for chronic toxicity testing, e.g., for integration into species sensitivity distribution (SSD) approaches. To address this gap, we performed a long-term 38-day semi-static toxicity test with the European mayfly species Cloeon dipterum using the insecticide fipronil as model substance. The functionality of the test system was confirmed by the high emergence rate of 85% in the control condition. We found a high sensitivity with regard to larval development with an EC50 of 180 ng/L and a NOEC of 38.0 ng/L after 7 days exposure. After 38 days, an LC50 value of 185 ng/L and an EC50 value of 160 ng/L for emergence (both: NOEC = 38.0 ng/L) were calculated. In a short-term 7-day toxicity test, we found a similar effect on larval development. In addition to the physiological endpoints, we examined fipronil-induced gene expression changes at the transcriptome level in this test. Our results revealed a concentration-dependent increase in the number of differentially expressed genes, as well as observed effects on larval development. Notably, we identified marker gene candidates involved in nervous system development, mirroring the known mode-of-action of fipronil in C. dipterum. The affected genes primarily play crucial roles in neurological processes. Concluding, within this two-step approach we were able to identify fipronil effects on the sublethal physiological endpoint larval development and to complement these effects at the molecular level by gene expression changes in the transcriptome. Thus, this assay proved to be suitable to assess sublethal effects as well as the mode-of-action of substances in the non-standard organism C. dipterum already after a short-term exposure of 7 days. However, further testing is required to validate the procedure. |
| first_indexed | 2024-03-10T22:09:52Z |
| format | Article |
| id | doaj.art-f262b4d176174a6c873cf577cc1fed85 |
| institution | Directory Open Access Journal |
| issn | 2190-4715 |
| language | English |
| last_indexed | 2024-03-10T22:09:52Z |
| publishDate | 2023-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Environmental Sciences Europe |
| spelling | doaj.art-f262b4d176174a6c873cf577cc1fed852023-11-19T12:40:35ZengSpringerOpenEnvironmental Sciences Europe2190-47152023-11-0135111510.1186/s12302-023-00806-4Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterumKirsten Germing0Steve U. Ayobahan1Hannes Reinwald2Maria Vogt3Benedikt Ringbeck4Bernd Göckener5Elke Eilebrecht6Lena Kosak7Sebastian Eilebrecht8Department of Ecotoxicology, Fraunhofer Institute for Molecular Biology and Applied EcologyFraunhofer Attract Eco‘n’OMICs, Fraunhofer Institute for Molecular Biology and Applied EcologyFraunhofer Attract Eco‘n’OMICs, Fraunhofer Institute for Molecular Biology and Applied EcologyDepartment of Ecotoxicology, Fraunhofer Institute for Molecular Biology and Applied EcologyDepartment of Trace Analysis and Environmental Monitoring, Fraunhofer Institute for Molecular Biology and Applied EcologyDepartment of Trace Analysis and Environmental Monitoring, Fraunhofer Institute for Molecular Biology and Applied EcologyDepartment of Ecotoxicology, Fraunhofer Institute for Molecular Biology and Applied EcologyDepartment of Ecotoxicology, Fraunhofer Institute for Molecular Biology and Applied EcologyFraunhofer Attract Eco‘n’OMICs, Fraunhofer Institute for Molecular Biology and Applied EcologyAbstract The so-called EPT taxa have been shown to be highly sensitive to various environmental pollutants. However, there are only few published studies on toxicity testing with EPT representatives and there is a particular lack of protocols for chronic toxicity testing, e.g., for integration into species sensitivity distribution (SSD) approaches. To address this gap, we performed a long-term 38-day semi-static toxicity test with the European mayfly species Cloeon dipterum using the insecticide fipronil as model substance. The functionality of the test system was confirmed by the high emergence rate of 85% in the control condition. We found a high sensitivity with regard to larval development with an EC50 of 180 ng/L and a NOEC of 38.0 ng/L after 7 days exposure. After 38 days, an LC50 value of 185 ng/L and an EC50 value of 160 ng/L for emergence (both: NOEC = 38.0 ng/L) were calculated. In a short-term 7-day toxicity test, we found a similar effect on larval development. In addition to the physiological endpoints, we examined fipronil-induced gene expression changes at the transcriptome level in this test. Our results revealed a concentration-dependent increase in the number of differentially expressed genes, as well as observed effects on larval development. Notably, we identified marker gene candidates involved in nervous system development, mirroring the known mode-of-action of fipronil in C. dipterum. The affected genes primarily play crucial roles in neurological processes. Concluding, within this two-step approach we were able to identify fipronil effects on the sublethal physiological endpoint larval development and to complement these effects at the molecular level by gene expression changes in the transcriptome. Thus, this assay proved to be suitable to assess sublethal effects as well as the mode-of-action of substances in the non-standard organism C. dipterum already after a short-term exposure of 7 days. However, further testing is required to validate the procedure.https://doi.org/10.1186/s12302-023-00806-4EPT taxaCloeon dipterumChronic toxicityFipronilGene expression changesTranscriptomics |
| spellingShingle | Kirsten Germing Steve U. Ayobahan Hannes Reinwald Maria Vogt Benedikt Ringbeck Bernd Göckener Elke Eilebrecht Lena Kosak Sebastian Eilebrecht Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum Environmental Sciences Europe EPT taxa Cloeon dipterum Chronic toxicity Fipronil Gene expression changes Transcriptomics |
| title | Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum |
| title_full | Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum |
| title_fullStr | Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum |
| title_full_unstemmed | Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum |
| title_short | Chronic toxicity testing including transcriptomics-based molecular profiling in Cloeon dipterum |
| title_sort | chronic toxicity testing including transcriptomics based molecular profiling in cloeon dipterum |
| topic | EPT taxa Cloeon dipterum Chronic toxicity Fipronil Gene expression changes Transcriptomics |
| url | https://doi.org/10.1186/s12302-023-00806-4 |
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