Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome
Purpose : Expression levels of tumor necrosis factor (TNF)-?#6181;xpression on the mucosa of the small intestine is increased in patients with villous atrophy in food protein-induced enterocolitis syndrome (FPIES). TNF-?#6184;as been reported to induce apoptotic cell death in the epithelial cells. W...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Pediatric Society
2010-04-01
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| Series: | Korean Journal of Pediatrics |
| Online Access: | http://www.kjp.or.kr/upload/2010530410-20100651180751.PDF |
| _version_ | 1818543021416775680 |
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| author | Jin-Bok Hwang Sang Pyo Kim Yu Na Kang Seong-Ryong Lee Seong-Il Suh Taeg Kyu Kwon |
| author_facet | Jin-Bok Hwang Sang Pyo Kim Yu Na Kang Seong-Ryong Lee Seong-Il Suh Taeg Kyu Kwon |
| author_sort | Jin-Bok Hwang |
| collection | DOAJ |
| description | Purpose : Expression levels of tumor necrosis factor (TNF)-?#6181;xpression on the mucosa of the small intestine is increased in patients with villous atrophy in food protein-induced enterocolitis syndrome (FPIES). TNF-?#6184;as been reported to induce apoptotic cell death in the epithelial cells. We studied the TNF family and TNF-receptor family apoptosis on the duodenal mucosa to investigate their roles in the pathogenesis of FPIES. Methods : Fifteen infants diagnosed as having FPIES using standard oral challenge test and 5 controls were included. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining was performed to identify the apoptotic cell death bodies. Immunohistochemical staining of TNF-?#6944;Fas ligand (FasL) for TNF family and TNF-related apoptosis-including ligand (TRAIL) receptor 1 (DR4), TRAIL receptor 2 (DR5), and Fas for TNF-receptor family were performed to determine the apoptotic mechanisms. Results : TUNEL+ was significantly more highly expressed in the duodenal mucosa of FPIES patients than in controls (P = 0.043). TNF-?#6184;P =0.0001) and DR4 (P =0.003) were significantly more highly expressed in FPIES patients than in controls. Expression levels of FasL, Fas, and DR5 were low in both groups and were not significantly different between the 2 groups. Conclusion : These results suggest that FPIES pathogenesis is induced by apoptosis, and that TNF-?#6181;xpression and DR4 pathway may have an important role in apoptosis. |
| first_indexed | 2024-12-11T22:29:49Z |
| format | Article |
| id | doaj.art-f263068f220947b0bfb4ce68fffe63f3 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-12-11T22:29:49Z |
| publishDate | 2010-04-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-f263068f220947b0bfb4ce68fffe63f32022-12-22T00:48:11ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582010-04-0153452553110.3345/kjp.2010.53.4.525Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndromeJin-Bok HwangSang Pyo KimYu Na KangSeong-Ryong LeeSeong-Il SuhTaeg Kyu KwonPurpose : Expression levels of tumor necrosis factor (TNF)-?#6181;xpression on the mucosa of the small intestine is increased in patients with villous atrophy in food protein-induced enterocolitis syndrome (FPIES). TNF-?#6184;as been reported to induce apoptotic cell death in the epithelial cells. We studied the TNF family and TNF-receptor family apoptosis on the duodenal mucosa to investigate their roles in the pathogenesis of FPIES. Methods : Fifteen infants diagnosed as having FPIES using standard oral challenge test and 5 controls were included. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining was performed to identify the apoptotic cell death bodies. Immunohistochemical staining of TNF-?#6944;Fas ligand (FasL) for TNF family and TNF-related apoptosis-including ligand (TRAIL) receptor 1 (DR4), TRAIL receptor 2 (DR5), and Fas for TNF-receptor family were performed to determine the apoptotic mechanisms. Results : TUNEL+ was significantly more highly expressed in the duodenal mucosa of FPIES patients than in controls (P = 0.043). TNF-?#6184;P =0.0001) and DR4 (P =0.003) were significantly more highly expressed in FPIES patients than in controls. Expression levels of FasL, Fas, and DR5 were low in both groups and were not significantly different between the 2 groups. Conclusion : These results suggest that FPIES pathogenesis is induced by apoptosis, and that TNF-?#6181;xpression and DR4 pathway may have an important role in apoptosis.http://www.kjp.or.kr/upload/2010530410-20100651180751.PDF |
| spellingShingle | Jin-Bok Hwang Sang Pyo Kim Yu Na Kang Seong-Ryong Lee Seong-Il Suh Taeg Kyu Kwon Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome Korean Journal of Pediatrics |
| title | Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome |
| title_full | Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome |
| title_fullStr | Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome |
| title_full_unstemmed | Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome |
| title_short | Apoptosis and upregulation of TNF-α and TRAIL receptor 1 (DR4) in the pathogenesis of food protein-induced enterocolitis syndrome |
| title_sort | apoptosis and upregulation of tnf α and trail receptor 1 dr4 in the pathogenesis of food protein induced enterocolitis syndrome |
| url | http://www.kjp.or.kr/upload/2010530410-20100651180751.PDF |
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