Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces
Fragrance, which plays an important role in determining the economic value of rice to growers and consumers, is known to be controlled by the <i>Badh2</i> gene. This study evaluated the grain quality characteristics and allelic variation of the <i>Badh2</i> gene in 22 fragran...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/6/779 |
| _version_ | 1827715960636702720 |
|---|---|
| author | Phukjira Chan-in Sansanee Jamjod Narit Yimyam Benjavan Rerkasem Tonapha Pusadee |
| author_facet | Phukjira Chan-in Sansanee Jamjod Narit Yimyam Benjavan Rerkasem Tonapha Pusadee |
| author_sort | Phukjira Chan-in |
| collection | DOAJ |
| description | Fragrance, which plays an important role in determining the economic value of rice to growers and consumers, is known to be controlled by the <i>Badh2</i> gene. This study evaluated the grain quality characteristics and allelic variation of the <i>Badh2</i> gene in 22 fragrant rice landraces from Thailand. The rice seed samples from farmers’ storage facilities in northern, northeastern and southern Thailand, plus two advanced breeding lines and three check varieties, were evaluated for seed morphology and grain quality, and their <i>Badh2</i> genes covering intron 4 to intron 8 were re-sequenced. Almost all of the landraces were classified as large grain types, with medium to high gelatinization temperatures. The variation in the <i>Badh2</i> gene by haplotype analysis correlated with grain aroma by sensory evaluation. The <i>badh2</i>-E7 was found in haplotype 1 with a strong aroma in KH, NDLP, and PLD, as in KDML105 and the moderately aromatic BNM-CMU, BNM4, and SKH, along with PTT1. Three haplotypes had different positions of SNP on the <i>Badh2</i> gene with varying results in the sensory test. The present results suggest that some rice varieties could be potentially introduced as genetic resources for fragrant rice breeding programs or could be developed to highly palatable cultivars with geographical indications to increase the income of highland farmers. |
| first_indexed | 2024-03-10T19:29:12Z |
| format | Article |
| id | doaj.art-f2652103ecc94b81bc2599e06c6fbeea |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T19:29:12Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-f2652103ecc94b81bc2599e06c6fbeea2023-11-20T02:18:17ZengMDPI AGAgronomy2073-43952020-05-0110677910.3390/agronomy10060779Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice LandracesPhukjira Chan-in0Sansanee Jamjod1Narit Yimyam2Benjavan Rerkasem3Tonapha Pusadee4Plant Genetic Resource and Nutrition lab (CMUPNlab), Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandPlant Genetic Resource and Nutrition lab (CMUPNlab), Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Highland Agriculture Natural Resources and Environment, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandPlant Genetic Resource and Nutrition lab (CMUPNlab), Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandPlant Genetic Resource and Nutrition lab (CMUPNlab), Department of Plant and Soil Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, ThailandFragrance, which plays an important role in determining the economic value of rice to growers and consumers, is known to be controlled by the <i>Badh2</i> gene. This study evaluated the grain quality characteristics and allelic variation of the <i>Badh2</i> gene in 22 fragrant rice landraces from Thailand. The rice seed samples from farmers’ storage facilities in northern, northeastern and southern Thailand, plus two advanced breeding lines and three check varieties, were evaluated for seed morphology and grain quality, and their <i>Badh2</i> genes covering intron 4 to intron 8 were re-sequenced. Almost all of the landraces were classified as large grain types, with medium to high gelatinization temperatures. The variation in the <i>Badh2</i> gene by haplotype analysis correlated with grain aroma by sensory evaluation. The <i>badh2</i>-E7 was found in haplotype 1 with a strong aroma in KH, NDLP, and PLD, as in KDML105 and the moderately aromatic BNM-CMU, BNM4, and SKH, along with PTT1. Three haplotypes had different positions of SNP on the <i>Badh2</i> gene with varying results in the sensory test. The present results suggest that some rice varieties could be potentially introduced as genetic resources for fragrant rice breeding programs or could be developed to highly palatable cultivars with geographical indications to increase the income of highland farmers.https://www.mdpi.com/2073-4395/10/6/779aromatic ricelocal varietygelatinization temperature<i>badh2</i>-E7 allele |
| spellingShingle | Phukjira Chan-in Sansanee Jamjod Narit Yimyam Benjavan Rerkasem Tonapha Pusadee Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces Agronomy aromatic rice local variety gelatinization temperature <i>badh2</i>-E7 allele |
| title | Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces |
| title_full | Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces |
| title_fullStr | Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces |
| title_full_unstemmed | Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces |
| title_short | Grain Quality and Allelic Variation of the <i>Badh2</i> Gene in Thai Fragrant Rice Landraces |
| title_sort | grain quality and allelic variation of the i badh2 i gene in thai fragrant rice landraces |
| topic | aromatic rice local variety gelatinization temperature <i>badh2</i>-E7 allele |
| url | https://www.mdpi.com/2073-4395/10/6/779 |
| work_keys_str_mv | AT phukjirachanin grainqualityandallelicvariationoftheibadh2igeneinthaifragrantricelandraces AT sansaneejamjod grainqualityandallelicvariationoftheibadh2igeneinthaifragrantricelandraces AT narityimyam grainqualityandallelicvariationoftheibadh2igeneinthaifragrantricelandraces AT benjavanrerkasem grainqualityandallelicvariationoftheibadh2igeneinthaifragrantricelandraces AT tonaphapusadee grainqualityandallelicvariationoftheibadh2igeneinthaifragrantricelandraces |