Genome-Wide Identification and Expression Analysis of NCED Gene Family in Pear and Its Response to Exogenous Gibberellin and Paclobutrazol
The 9-<i>cis</i>-epoxycarotenoid dioxygenase (<i>NCED</i>) is a key enzyme for the process of ABA synthesis that plays key roles in a variety of biological processes. In the current investigation, genome-wide identification and comprehensive analysis of the <i>NCED</...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/8/7566 |
| Summary: | The 9-<i>cis</i>-epoxycarotenoid dioxygenase (<i>NCED</i>) is a key enzyme for the process of ABA synthesis that plays key roles in a variety of biological processes. In the current investigation, genome-wide identification and comprehensive analysis of the <i>NCED</i> gene family in ‘Kuerle Xiangli’ (<i>Pyrus sinkiangensis</i> Yu) were conducted using the pear genomic sequence. In total, nineteen members of <i>PbNCED</i> genes were identified from the whole genome of pear, which are not evenly distributed over the scaffolds, and most of which were focussed in the chloroplasts. Sequence analysis of promoters showed many <i>cis</i>-regulatory elements, which presumably responded to phytohormones such as abscisic acid, auxin, etc. Synteny block indicated that the <i>PbNCED</i> genes have experienced strong purifying selection. Multiple sequence alignment demonstrated that these members are highly similar and conserved. In addition, we found that <i>PbNCED</i> genes were differentially expressed in various tissues, and three <i>PbNCED</i> genes (<i>PbNCED1</i>, <i>PbNCED2</i>, and <i>PbNCED13</i>) were differentially expressed in response to exogenous Gibberellin (GA<sub>3</sub>) and Paclobutrazol (PP<sub>333</sub>). <i>PbNCED1</i> and <i>PbNCED13</i> positively promote ABA synthesis in sepals after GA<sub>3</sub> and PP<sub>333</sub> treatment, whereas <i>PbNCED2</i> positively regulated ABA synthesis in ovaries after GA<sub>3</sub> treatment, and <i>PbNCED13</i> positively regulated ABA synthesis in the ovaries after PP<sub>333</sub> treatment. This study was the first genome-wide report of the pear <i>NCED</i> gene family, which could improve our understanding of pear NCED proteins and provide a solid foundation for future cloning and functional analyses of this gene family. Meanwhile, our results also give a better understanding of the important genes and regulation pathways related to calyx abscission in ‘Kuerle Xiangli’. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |