Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology
The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expe...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2015-06-01
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| Series: | Makara Journal of Science |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/science/article/view/4776/3331 |
| _version_ | 1811255874934538240 |
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| author | Trismilah Trismilah Astutiati Nurhasanah Wahono Sumaryono Amarila Malik Mohamad Sadikin |
| author_facet | Trismilah Trismilah Astutiati Nurhasanah Wahono Sumaryono Amarila Malik Mohamad Sadikin |
| author_sort | Trismilah Trismilah |
| collection | DOAJ |
| description | The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5 Fermentation was carried out in erlenmeyer flasks at initial pH 8, 37 °C, using an incubator shaker at 87.5 rpm. The best results showed an enzyme activity of 1.0 mU/mL, a protein level of 0.557 mg/mL, and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or the desirability value of 1. The medium composition containing of 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk was used for the fermentation. The enzyme activity of 1.51 mU/mL and protein level of 0.205 mg/mL can be achieved. After numerical optimization, the fermentation process was verified in erlenmeyer flasks with incubator shaking at 77 rpm, initial pH 8, 37 °C, and 15 h fermentation. The verification results showed that the enzyme activity of 1.273 ± 0.227 mU/mL and protein level of 0.248 ± 0.012 mg/mL. |
| first_indexed | 2024-04-12T17:31:34Z |
| format | Article |
| id | doaj.art-f2708b6154c84aa397fa2d6a25b9811a |
| institution | Directory Open Access Journal |
| issn | 2339-1995 2356-0851 |
| language | English |
| last_indexed | 2024-04-12T17:31:34Z |
| publishDate | 2015-06-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Science |
| spelling | doaj.art-f2708b6154c84aa397fa2d6a25b9811a2022-12-22T03:23:07ZengUniversitas IndonesiaMakara Journal of Science2339-19952356-08512015-06-01192647210.7454/mss.v19i2.4776Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface MethodologyTrismilah Trismilah0Astutiati Nurhasanah1Wahono Sumaryono2Amarila Malik3Mohamad Sadikin4Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaLAPTIAB, Badan Pengkajian dan Penerapan Teknologi, Tangerang 15314, IndonesiaFaculty of Pharmacy, Universitas Indonesia, Depok 16424, IndonesiaFaculty of Pharmacy, Universitas Indonesia, Depok 16424, IndonesiaFaculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia The purpose of this study was to get optimum medium composition and agitation to trypsin-like protease production by Lactobacillus plantarum FNCC 0270. The medium composition and agitation for enzyme production was optimized using Central Composite Design and Response Surface Method with Design Expert software version 7.1.5 Fermentation was carried out in erlenmeyer flasks at initial pH 8, 37 °C, using an incubator shaker at 87.5 rpm. The best results showed an enzyme activity of 1.0 mU/mL, a protein level of 0.557 mg/mL, and desirability value of 0.740. Numerical optimization was performed to approach the ideal state of the fermentation or the desirability value of 1. The medium composition containing of 3.64% baker's yeast, 1.21% glucose, and 0.13% skim milk was used for the fermentation. The enzyme activity of 1.51 mU/mL and protein level of 0.205 mg/mL can be achieved. After numerical optimization, the fermentation process was verified in erlenmeyer flasks with incubator shaking at 77 rpm, initial pH 8, 37 °C, and 15 h fermentation. The verification results showed that the enzyme activity of 1.273 ± 0.227 mU/mL and protein level of 0.248 ± 0.012 mg/mL.http://journal.ui.ac.id/index.php/science/article/view/4776/3331central composite design enzyme activityLactobacillus plantarumprotein contentresponse surface methodology |
| spellingShingle | Trismilah Trismilah Astutiati Nurhasanah Wahono Sumaryono Amarila Malik Mohamad Sadikin Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology Makara Journal of Science central composite design enzyme activity Lactobacillus plantarum protein content response surface methodology |
| title | Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology |
| title_full | Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology |
| title_fullStr | Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology |
| title_full_unstemmed | Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology |
| title_short | Optimization of Trypsin-like Protease Production by Lactobacillus plantarum FNCC 0270 using Response Surface Methodology |
| title_sort | optimization of trypsin like protease production by lactobacillus plantarum fncc 0270 using response surface methodology |
| topic | central composite design enzyme activity Lactobacillus plantarum protein content response surface methodology |
| url | http://journal.ui.ac.id/index.php/science/article/view/4776/3331 |
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