New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs.
Current knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the gene...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3935994?pdf=render |
| _version_ | 1818970733217316864 |
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| author | Isabel Natalia Sierra-García Javier Correa Alvarez Suzan Pantaroto de Vasconcellos Anete Pereira de Souza Eugenio Vaz dos Santos Neto Valéria Maia de Oliveira |
| author_facet | Isabel Natalia Sierra-García Javier Correa Alvarez Suzan Pantaroto de Vasconcellos Anete Pereira de Souza Eugenio Vaz dos Santos Neto Valéria Maia de Oliveira |
| author_sort | Isabel Natalia Sierra-García |
| collection | DOAJ |
| description | Current knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the genetic and functional diversity of previously uncultured microorganisms in natural environments. Using a function-driven metagenomic approach, we investigated the metabolic abilities of microbial communities in oil reservoirs. Here, we describe novel functional metabolic pathways involved in the biodegradation of aromatic compounds in a metagenomic library obtained from an oil reservoir. Although many of the deduced proteins shared homology with known enzymes of different well-described aerobic and anaerobic catabolic pathways, the metagenomic fragments did not contain the complete clusters known to be involved in hydrocarbon degradation. Instead, the metagenomic fragments comprised genes belonging to different pathways, showing novel gene arrangements. These results reinforce the potential of the metagenomic approach for the identification and elucidation of new genes and pathways in poorly studied environments and contribute to a broader perspective on the hydrocarbon degradation processes in petroleum reservoirs. |
| first_indexed | 2024-12-20T14:41:10Z |
| format | Article |
| id | doaj.art-f270cf153ca24509a2241eae17757293 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T14:41:10Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f270cf153ca24509a2241eae177572932022-12-21T19:37:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e9008710.1371/journal.pone.0090087New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs.Isabel Natalia Sierra-GarcíaJavier Correa AlvarezSuzan Pantaroto de VasconcellosAnete Pereira de SouzaEugenio Vaz dos Santos NetoValéria Maia de OliveiraCurrent knowledge of the microbial diversity and metabolic pathways involved in hydrocarbon degradation in petroleum reservoirs is still limited, mostly due to the difficulty in recovering the complex community from such an extreme environment. Metagenomics is a valuable tool to investigate the genetic and functional diversity of previously uncultured microorganisms in natural environments. Using a function-driven metagenomic approach, we investigated the metabolic abilities of microbial communities in oil reservoirs. Here, we describe novel functional metabolic pathways involved in the biodegradation of aromatic compounds in a metagenomic library obtained from an oil reservoir. Although many of the deduced proteins shared homology with known enzymes of different well-described aerobic and anaerobic catabolic pathways, the metagenomic fragments did not contain the complete clusters known to be involved in hydrocarbon degradation. Instead, the metagenomic fragments comprised genes belonging to different pathways, showing novel gene arrangements. These results reinforce the potential of the metagenomic approach for the identification and elucidation of new genes and pathways in poorly studied environments and contribute to a broader perspective on the hydrocarbon degradation processes in petroleum reservoirs.http://europepmc.org/articles/PMC3935994?pdf=render |
| spellingShingle | Isabel Natalia Sierra-García Javier Correa Alvarez Suzan Pantaroto de Vasconcellos Anete Pereira de Souza Eugenio Vaz dos Santos Neto Valéria Maia de Oliveira New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. PLoS ONE |
| title | New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. |
| title_full | New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. |
| title_fullStr | New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. |
| title_full_unstemmed | New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. |
| title_short | New hydrocarbon degradation pathways in the microbial metagenome from Brazilian petroleum reservoirs. |
| title_sort | new hydrocarbon degradation pathways in the microbial metagenome from brazilian petroleum reservoirs |
| url | http://europepmc.org/articles/PMC3935994?pdf=render |
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