The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies
Natural genetic variation in photosynthesis is strictly associated with the remarkable adaptive plasticity observed amongst <i>Arabidopsis thaliana</i> accessions derived from environmentally distinct regions. Exploration of the characteristic features of the photosynthetic machinery cou...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/18/9866 |
| _version_ | 1797518932646559744 |
|---|---|
| author | Joanna Wójtowicz Katarzyna B. Gieczewska |
| author_facet | Joanna Wójtowicz Katarzyna B. Gieczewska |
| author_sort | Joanna Wójtowicz |
| collection | DOAJ |
| description | Natural genetic variation in photosynthesis is strictly associated with the remarkable adaptive plasticity observed amongst <i>Arabidopsis thaliana</i> accessions derived from environmentally distinct regions. Exploration of the characteristic features of the photosynthetic machinery could reveal the regulatory mechanisms underlying those traits. In this study, we performed a detailed characterisation and comparison of photosynthesis performance and spectral properties of the photosynthetic apparatus in the following selected <i>Arabidopsis thaliana</i> accessions commonly used in laboratories as background lines: Col-0, Col-1, Col-2, Col-8, Ler-0, and Ws-2. The main focus was to distinguish the characteristic disparities for every accession in photosynthetic efficiency that could be accountable for their remarkable plasticity to adapt. The biophysical and biochemical analysis of the thylakoid membranes in control conditions revealed differences in lipid-to-protein contribution, Chlorophyll-to-Carotenoid ratio (Chl/Car), and xanthophyll cycle pigment distribution among accessions. We presented that such changes led to disparities in the arrangement of the Chlorophyll-Protein complexes, the PSI/PSII ratio, and the lateral mobility of the thylakoid membrane, with the most significant aberrations detected in the Ler-0 and Ws-2 accessions. We concluded that selecting an accession suitable for specific research on the photosynthetic process is essential for optimising the experiment. |
| first_indexed | 2024-03-10T07:36:18Z |
| format | Article |
| id | doaj.art-f2730445be3a4e7b9cf10e464c4aabee |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T07:36:18Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f2730445be3a4e7b9cf10e464c4aabee2023-11-22T13:28:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-012218986610.3390/ijms22189866The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic StudiesJoanna Wójtowicz0Katarzyna B. Gieczewska1Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, PolandDepartment of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, I. Miecznikowa 1, 02-096 Warsaw, PolandNatural genetic variation in photosynthesis is strictly associated with the remarkable adaptive plasticity observed amongst <i>Arabidopsis thaliana</i> accessions derived from environmentally distinct regions. Exploration of the characteristic features of the photosynthetic machinery could reveal the regulatory mechanisms underlying those traits. In this study, we performed a detailed characterisation and comparison of photosynthesis performance and spectral properties of the photosynthetic apparatus in the following selected <i>Arabidopsis thaliana</i> accessions commonly used in laboratories as background lines: Col-0, Col-1, Col-2, Col-8, Ler-0, and Ws-2. The main focus was to distinguish the characteristic disparities for every accession in photosynthetic efficiency that could be accountable for their remarkable plasticity to adapt. The biophysical and biochemical analysis of the thylakoid membranes in control conditions revealed differences in lipid-to-protein contribution, Chlorophyll-to-Carotenoid ratio (Chl/Car), and xanthophyll cycle pigment distribution among accessions. We presented that such changes led to disparities in the arrangement of the Chlorophyll-Protein complexes, the PSI/PSII ratio, and the lateral mobility of the thylakoid membrane, with the most significant aberrations detected in the Ler-0 and Ws-2 accessions. We concluded that selecting an accession suitable for specific research on the photosynthetic process is essential for optimising the experiment.https://www.mdpi.com/1422-0067/22/18/9866<i>Arabidopsis thaliana</i> accessionsCol-0Col-1Col-2Col-8Ler-0 |
| spellingShingle | Joanna Wójtowicz Katarzyna B. Gieczewska The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> accessions Col-0 Col-1 Col-2 Col-8 Ler-0 |
| title | The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies |
| title_full | The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies |
| title_fullStr | The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies |
| title_full_unstemmed | The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies |
| title_short | The Arabidopsis Accessions Selection Is Crucial: Insight from Photosynthetic Studies |
| title_sort | arabidopsis accessions selection is crucial insight from photosynthetic studies |
| topic | <i>Arabidopsis thaliana</i> accessions Col-0 Col-1 Col-2 Col-8 Ler-0 |
| url | https://www.mdpi.com/1422-0067/22/18/9866 |
| work_keys_str_mv | AT joannawojtowicz thearabidopsisaccessionsselectioniscrucialinsightfromphotosyntheticstudies AT katarzynabgieczewska thearabidopsisaccessionsselectioniscrucialinsightfromphotosyntheticstudies AT joannawojtowicz arabidopsisaccessionsselectioniscrucialinsightfromphotosyntheticstudies AT katarzynabgieczewska arabidopsisaccessionsselectioniscrucialinsightfromphotosyntheticstudies |