A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein
The ongoing COVID-19 pandemic caused by SARS-CoV-2 has posed a devastating threat worldwide. The receptor-binding domain (RBD) of the spike protein is one of the most important antigens for SARS-CoV-2 vaccines, while the analysis of CD8 cytotoxic T lymphocyte activity in preclinical studies using mo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/6/641 |
| _version_ | 1797530564005199872 |
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| author | Jihyun Yang Eunjin Kim Jong-Soo Lee Haryoung Poo |
| author_facet | Jihyun Yang Eunjin Kim Jong-Soo Lee Haryoung Poo |
| author_sort | Jihyun Yang |
| collection | DOAJ |
| description | The ongoing COVID-19 pandemic caused by SARS-CoV-2 has posed a devastating threat worldwide. The receptor-binding domain (RBD) of the spike protein is one of the most important antigens for SARS-CoV-2 vaccines, while the analysis of CD8 cytotoxic T lymphocyte activity in preclinical studies using mouse models is critical for evaluating vaccine efficacy. Here, we immunized C57BL/6 wild-type mice and transgenic mice expressing human angiotensin-converting enzyme 2 (ACE2) with the SARS-CoV-2 RBD protein to evaluate the IFN-γ-producing T cells in the splenocytes of the immunized mice using an overlapping peptide pool by an enzyme-linked immunospot assay and flow cytometry. We identified SARS-CoV-2 S<sub>395–404</sub> as a major histocompatibility complex (MHC) class I-restricted epitope for the RBD-specific CD8 T cell responses in C57BL/6 mice. |
| first_indexed | 2024-03-10T10:30:48Z |
| format | Article |
| id | doaj.art-f2741c6de1e74a468ff44b84a6e33202 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T10:30:48Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-f2741c6de1e74a468ff44b84a6e332022023-11-21T23:42:07ZengMDPI AGVaccines2076-393X2021-06-019664110.3390/vaccines9060641A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike ProteinJihyun Yang0Eunjin Kim1Jong-Soo Lee2Haryoung Poo3Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaDepartment of Preventive Veterinary Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, KoreaThe ongoing COVID-19 pandemic caused by SARS-CoV-2 has posed a devastating threat worldwide. The receptor-binding domain (RBD) of the spike protein is one of the most important antigens for SARS-CoV-2 vaccines, while the analysis of CD8 cytotoxic T lymphocyte activity in preclinical studies using mouse models is critical for evaluating vaccine efficacy. Here, we immunized C57BL/6 wild-type mice and transgenic mice expressing human angiotensin-converting enzyme 2 (ACE2) with the SARS-CoV-2 RBD protein to evaluate the IFN-γ-producing T cells in the splenocytes of the immunized mice using an overlapping peptide pool by an enzyme-linked immunospot assay and flow cytometry. We identified SARS-CoV-2 S<sub>395–404</sub> as a major histocompatibility complex (MHC) class I-restricted epitope for the RBD-specific CD8 T cell responses in C57BL/6 mice.https://www.mdpi.com/2076-393X/9/6/641SARS-CoV-2cell-mediated immunityCD8 cytotoxic T lymphocyteepitopevaccine |
| spellingShingle | Jihyun Yang Eunjin Kim Jong-Soo Lee Haryoung Poo A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein Vaccines SARS-CoV-2 cell-mediated immunity CD8 cytotoxic T lymphocyte epitope vaccine |
| title | A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein |
| title_full | A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein |
| title_fullStr | A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein |
| title_full_unstemmed | A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein |
| title_short | A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein |
| title_sort | murine cd8 sup sup t cell epitope identified in the receptor binding domain of the sars cov 2 spike protein |
| topic | SARS-CoV-2 cell-mediated immunity CD8 cytotoxic T lymphocyte epitope vaccine |
| url | https://www.mdpi.com/2076-393X/9/6/641 |
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