Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain
In many clinical cases of radicular pain, no noticeable neuropathology is detected by conventional medical imaging strategies. Superparamagnetic iron oxide (SPIO) nanoparticles were evaluated as magnetic resonance contrast agents to specifically detect neuroinflammation at sites of painful injury in...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publications
2011-05-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2010.00042 |
| _version_ | 1797285303458725888 |
|---|---|
| author | Daniel L.J. Thorek Christine L. Weisshaar Julie C. Czupryna Beth A. Winkelstein Andrew Tsourkas |
| author_facet | Daniel L.J. Thorek Christine L. Weisshaar Julie C. Czupryna Beth A. Winkelstein Andrew Tsourkas |
| author_sort | Daniel L.J. Thorek |
| collection | DOAJ |
| description | In many clinical cases of radicular pain, no noticeable neuropathology is detected by conventional medical imaging strategies. Superparamagnetic iron oxide (SPIO) nanoparticles were evaluated as magnetic resonance contrast agents to specifically detect neuroinflammation at sites of painful injury in a rat model of cervical nerve root compression. Two separate groups of rats were used: an injury group that underwent controlled transient compression of the dorsal root and a sham group that received the same surgical procedures but no injury. Precontrast magnetic resonance imaging (MRI) was performed 6 days after surgery, followed by administration of SPIO via tail vein injection. After 24 hours, T 2 * -weighted imaging at the site of root injury revealed a postcontrast enhancement of 72.9 ± 31%. This was significantly greater than that of injured animals prior to SPIO administration (5.3 ± 12.9%). SPIO did not generate any significant postcontrast enhancement in the nerve roots of the sham group. Histology confirmed colocalization of SPIO with macrophage at the injury site. These findings suggest that SPIO-enhanced MRI may be a valuable tool to identify otherwise undetectable nerve root compression and enable improved patient management. |
| first_indexed | 2024-03-07T18:01:12Z |
| format | Article |
| id | doaj.art-f276910f3678411f8216f0eabe3e7378 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:01:12Z |
| publishDate | 2011-05-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-f276910f3678411f8216f0eabe3e73782024-03-02T10:53:01ZengSAGE PublicationsMolecular Imaging1536-01212011-05-011010.2310/7290.2010.0004210.2310_7290.2010.00042Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular PainDaniel L.J. ThorekChristine L. WeisshaarJulie C. CzuprynaBeth A. WinkelsteinAndrew TsourkasIn many clinical cases of radicular pain, no noticeable neuropathology is detected by conventional medical imaging strategies. Superparamagnetic iron oxide (SPIO) nanoparticles were evaluated as magnetic resonance contrast agents to specifically detect neuroinflammation at sites of painful injury in a rat model of cervical nerve root compression. Two separate groups of rats were used: an injury group that underwent controlled transient compression of the dorsal root and a sham group that received the same surgical procedures but no injury. Precontrast magnetic resonance imaging (MRI) was performed 6 days after surgery, followed by administration of SPIO via tail vein injection. After 24 hours, T 2 * -weighted imaging at the site of root injury revealed a postcontrast enhancement of 72.9 ± 31%. This was significantly greater than that of injured animals prior to SPIO administration (5.3 ± 12.9%). SPIO did not generate any significant postcontrast enhancement in the nerve roots of the sham group. Histology confirmed colocalization of SPIO with macrophage at the injury site. These findings suggest that SPIO-enhanced MRI may be a valuable tool to identify otherwise undetectable nerve root compression and enable improved patient management.https://doi.org/10.2310/7290.2010.00042 |
| spellingShingle | Daniel L.J. Thorek Christine L. Weisshaar Julie C. Czupryna Beth A. Winkelstein Andrew Tsourkas Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain Molecular Imaging |
| title | Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain |
| title_full | Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain |
| title_fullStr | Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain |
| title_full_unstemmed | Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain |
| title_short | Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain |
| title_sort | superparamagnetic iron oxide enhanced magnetic resonance imaging of neuroinflammation in a rat model of radicular pain |
| url | https://doi.org/10.2310/7290.2010.00042 |
| work_keys_str_mv | AT danielljthorek superparamagneticironoxideenhancedmagneticresonanceimagingofneuroinflammationinaratmodelofradicularpain AT christinelweisshaar superparamagneticironoxideenhancedmagneticresonanceimagingofneuroinflammationinaratmodelofradicularpain AT juliecczupryna superparamagneticironoxideenhancedmagneticresonanceimagingofneuroinflammationinaratmodelofradicularpain AT bethawinkelstein superparamagneticironoxideenhancedmagneticresonanceimagingofneuroinflammationinaratmodelofradicularpain AT andrewtsourkas superparamagneticironoxideenhancedmagneticresonanceimagingofneuroinflammationinaratmodelofradicularpain |