Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats
In this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as follows...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2017-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2061 |
| _version_ | 1797800605759045632 |
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| author | BAKIR B KARADAG SARI E ELIS YILDIZ S ASKER H |
| author_facet | BAKIR B KARADAG SARI E ELIS YILDIZ S ASKER H |
| author_sort | BAKIR B |
| collection | DOAJ |
| description | In this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of
Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as
follows: control, sham and thymoquinone. While Thymoquinone group received 8 mg/kg of TQ which injected for 14 days, only isotonic saline was
injected to the sham group for same time. The control group received nothing. Crossman"s triple staining was applied to tissue sections to examine
histology. Streptavidin-Biotin-Peroxidase Complex method was used to investigate somatostatin immunoreactivity in the pancreatic tissue. Specific
somatostatin immunoreactivities were observed in endocrine cells (islets of Langerhans) in all groups. It was determined that somatostatin secretion
increased in the thymoquinone group compared to control and sham. In conclusion, thymoquinone administration was concluded to increase the
secretion of somatostatin that is known to regulate certain hormones such as growth hormone, insulin, glucagon, secretin and gastrin. |
| first_indexed | 2024-03-13T04:37:50Z |
| format | Article |
| id | doaj.art-f2809dcfbb98410c9aa546c4be7437ca |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:50Z |
| publishDate | 2017-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-f2809dcfbb98410c9aa546c4be7437ca2023-06-19T06:45:54ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-05-0123340941310.9775/kvfd.2016.168932061Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of ratsBAKIR B0KARADAG SARI E1ELIS YILDIZ S2ASKER H3Department of Histology and Embryology, Faculty of Veterinary Medicine, Namik Kemal University, TR-59030 Tekirdag - TURKEYDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, TR-36040 Kars - TURKEYSchool of Health Sciences, Kafkas University, TR-36040 Kars - TURKEYDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, TR-36040 Kars - TURKEYIn this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as follows: control, sham and thymoquinone. While Thymoquinone group received 8 mg/kg of TQ which injected for 14 days, only isotonic saline was injected to the sham group for same time. The control group received nothing. Crossman"s triple staining was applied to tissue sections to examine histology. Streptavidin-Biotin-Peroxidase Complex method was used to investigate somatostatin immunoreactivity in the pancreatic tissue. Specific somatostatin immunoreactivities were observed in endocrine cells (islets of Langerhans) in all groups. It was determined that somatostatin secretion increased in the thymoquinone group compared to control and sham. In conclusion, thymoquinone administration was concluded to increase the secretion of somatostatin that is known to regulate certain hormones such as growth hormone, insulin, glucagon, secretin and gastrin.https://vetdergikafkas.org/pdf.php?id=2061imunohistochemistrynigella sativapancreasthymoquinone |
| spellingShingle | BAKIR B KARADAG SARI E ELIS YILDIZ S ASKER H Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats Kafkas Universitesi Veteriner Fakültesi Dergisi imunohistochemistry nigella sativa pancreas thymoquinone |
| title | Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| title_full | Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| title_fullStr | Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| title_full_unstemmed | Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| title_short | Effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| title_sort | effects of thymoquinone supplementation on somatostatin secretion in pancreas tissue of rats |
| topic | imunohistochemistry nigella sativa pancreas thymoquinone |
| url | https://vetdergikafkas.org/pdf.php?id=2061 |
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