Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model
Objective(s): This study aimed to find out the protective effects and preliminary mechanisms of the flower extract of Caragana sinica (FEC) on dextran sulfate sodium salt (DSS)-induced colitis.Materials and Methods: The ulcerative colitis models of mice induced by 3% DSS were established and treated...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_17947_e7afbd39f52d07410d8ff8c8be44f65d.pdf |
| _version_ | 1819292496420667392 |
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| author | Ting Li Qiu-ping Zou Feng Huang Gui-guang Cheng Ze-wei Mao Ting Wang Fa-wu Dong Bao-jing Li Hong-ping He Yan-ping Li |
| author_facet | Ting Li Qiu-ping Zou Feng Huang Gui-guang Cheng Ze-wei Mao Ting Wang Fa-wu Dong Bao-jing Li Hong-ping He Yan-ping Li |
| author_sort | Ting Li |
| collection | DOAJ |
| description | Objective(s): This study aimed to find out the protective effects and preliminary mechanisms of the flower extract of Caragana sinica (FEC) on dextran sulfate sodium salt (DSS)-induced colitis.Materials and Methods: The ulcerative colitis models of mice induced by 3% DSS were established and treated with FEC. Body weight changes, disease activity index (DAI), colon histopathological score, anti-oxidant ability, and the level of inflammatory cytokines were determined. The expression of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) were assessed in colonic tissue by immunohistochemical staining. Western blot was used to analyze the expression of TLR4/ nuclear factor kappa-B (NF-κB) and TLR4/ mitogen-activated protein kinase (MAPK) signaling pathway-related proteins. Results: FEC significantly prevented body weight loss and colonic shortening and reduced the disease activity index and histopathological score (p <0.05). Moreover, FEC treatment remarkably down-regulated the levels of myeloperoxidase (MPO), interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) and up-regulated the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and interleukin 10 (IL-10) in the colon of DSS mice (p <0.05). Furthermore, the expression of TLR4/NF-κB and TLR4/MAPK pathway-related proteins was inhibited by FEC (p <0.05). Conclusion: Our findings demonstrated that FEC could serve as a potential therapeutic agent for treatment of ulcerative colitis. |
| first_indexed | 2024-12-24T03:55:27Z |
| format | Article |
| id | doaj.art-f283c4638a304df695fd686894df458f |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-24T03:55:27Z |
| publishDate | 2021-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-f283c4638a304df695fd686894df458f2022-12-21T17:16:28ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-05-0124559560310.22038/ijbms.2021.53847.1210617947Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse modelTing Li0Qiu-ping Zou1Feng Huang2Gui-guang Cheng3Ze-wei Mao4Ting Wang5Fa-wu Dong6Bao-jing Li7Hong-ping He8Yan-ping Li9College of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaYunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaCollege of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, People’s Republic of ChinaObjective(s): This study aimed to find out the protective effects and preliminary mechanisms of the flower extract of Caragana sinica (FEC) on dextran sulfate sodium salt (DSS)-induced colitis.Materials and Methods: The ulcerative colitis models of mice induced by 3% DSS were established and treated with FEC. Body weight changes, disease activity index (DAI), colon histopathological score, anti-oxidant ability, and the level of inflammatory cytokines were determined. The expression of Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 (MyD88) were assessed in colonic tissue by immunohistochemical staining. Western blot was used to analyze the expression of TLR4/ nuclear factor kappa-B (NF-κB) and TLR4/ mitogen-activated protein kinase (MAPK) signaling pathway-related proteins. Results: FEC significantly prevented body weight loss and colonic shortening and reduced the disease activity index and histopathological score (p <0.05). Moreover, FEC treatment remarkably down-regulated the levels of myeloperoxidase (MPO), interleukin-1beta (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6) and up-regulated the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and interleukin 10 (IL-10) in the colon of DSS mice (p <0.05). Furthermore, the expression of TLR4/NF-κB and TLR4/MAPK pathway-related proteins was inhibited by FEC (p <0.05). Conclusion: Our findings demonstrated that FEC could serve as a potential therapeutic agent for treatment of ulcerative colitis.https://ijbms.mums.ac.ir/article_17947_e7afbd39f52d07410d8ff8c8be44f65d.pdfflower extract of caragana sinicamapksnf-κbtlr4ulcerative colitis |
| spellingShingle | Ting Li Qiu-ping Zou Feng Huang Gui-guang Cheng Ze-wei Mao Ting Wang Fa-wu Dong Bao-jing Li Hong-ping He Yan-ping Li Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model Iranian Journal of Basic Medical Sciences flower extract of caragana sinica mapks nf-κb tlr4 ulcerative colitis |
| title | Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model |
| title_full | Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model |
| title_fullStr | Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model |
| title_full_unstemmed | Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model |
| title_short | Flower extract of Caragana sinica. ameliorates Dss-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model |
| title_sort | flower extract of caragana sinica ameliorates dss induced ulcerative colitis by affecting tlr4 nf κb and tlr4 mapk signaling pathway in a mouse model |
| topic | flower extract of caragana sinica mapks nf-κb tlr4 ulcerative colitis |
| url | https://ijbms.mums.ac.ir/article_17947_e7afbd39f52d07410d8ff8c8be44f65d.pdf |
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