Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress
Salinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three <i>Medicago truncatula</i> genotypes with differing...
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/269 |
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| author | Chrystalla Antoniou Xavier Zarza Gholamreza Gohari Sima Panahirad Panagiota Filippou Antonio F. Tiburcio Vasileios Fotopoulos |
| author_facet | Chrystalla Antoniou Xavier Zarza Gholamreza Gohari Sima Panahirad Panagiota Filippou Antonio F. Tiburcio Vasileios Fotopoulos |
| author_sort | Chrystalla Antoniou |
| collection | DOAJ |
| description | Salinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three <i>Medicago truncatula</i> genotypes with differing sensitivities to salinity (TN1.11, tolerant; Jemalong A17, moderately sensitive; TN6.18, sensitive), with the aim of examining the genotype-specific involvement of the polyamine metabolic pathway in plant response to salinity. The study was carried out with leaves harvested 48 h after watering plants with 200 mM NaCl. A comprehensive profile of free polyamines was determined using high performance liquid chromatography. All genotypes showed spermidine and spermine as the most abundant polyamines under control conditions. In salinity conditions, spermine levels increased at the expense of putrescine and spermidine, indicating a drift of polyamine metabolism towards the synthesis of increasing polycationic forms as a stress response. The increasing balance between high and low polycationic forms was clearly diminished in the salt-sensitive genotype TN6.18, showing a clear correlation with its sensitive phenotype. The polyamine metabolic profile was then supported by molecular evidence through the examination of polyamine metabolism transcript levels by RT-qPCR. General suppression of genes that are involved upstream in the PA biosynthetic pathway was determined. Contrarily, an induction in the expression of genes involved in the biosynthesis of spermine and spermidine was observed, in agreement with the metabolic analysis. A significant induction in diamino oxidase expression, involved in the catabolism of putrescine, was specifically found in the sensitive genotype ΤΝ6.18, indicating a distinct metabolic response to stress. Present findings highlight the involvement of polyamines in the defense response of <i>Medicago</i> genotypes showing sensitivity to salt stress. |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:16:31Z |
| publishDate | 2021-01-01 |
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| series | Plants |
| spelling | doaj.art-f28499ec20a44e6099d93ab91245b92f2023-12-03T15:21:46ZengMDPI AGPlants2223-77472021-01-0110226910.3390/plants10020269Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt StressChrystalla Antoniou0Xavier Zarza1Gholamreza Gohari2Sima Panahirad3Panagiota Filippou4Antonio F. Tiburcio5Vasileios Fotopoulos6Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology Limassol, Limassol 3036, CyprusPolyamine’s Laboratory, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainDepartment of Horticultural Sciences, Faculty of Agriculture, University of Maragheh, 83111-55181 Maragheh, IranDepartment of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, 51666-16471 Tabriz, IranDepartment of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology Limassol, Limassol 3036, CyprusPolyamine’s Laboratory, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, University of Barcelona, 08028 Barcelona, SpainDepartment of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology Limassol, Limassol 3036, CyprusSalinity constitutes one of the most important causes leading to severe reduction in plant yield. Several reports correlate the accumulation of polyamines in plants with tolerance to abiotic stress cues. The present study examined three <i>Medicago truncatula</i> genotypes with differing sensitivities to salinity (TN1.11, tolerant; Jemalong A17, moderately sensitive; TN6.18, sensitive), with the aim of examining the genotype-specific involvement of the polyamine metabolic pathway in plant response to salinity. The study was carried out with leaves harvested 48 h after watering plants with 200 mM NaCl. A comprehensive profile of free polyamines was determined using high performance liquid chromatography. All genotypes showed spermidine and spermine as the most abundant polyamines under control conditions. In salinity conditions, spermine levels increased at the expense of putrescine and spermidine, indicating a drift of polyamine metabolism towards the synthesis of increasing polycationic forms as a stress response. The increasing balance between high and low polycationic forms was clearly diminished in the salt-sensitive genotype TN6.18, showing a clear correlation with its sensitive phenotype. The polyamine metabolic profile was then supported by molecular evidence through the examination of polyamine metabolism transcript levels by RT-qPCR. General suppression of genes that are involved upstream in the PA biosynthetic pathway was determined. Contrarily, an induction in the expression of genes involved in the biosynthesis of spermine and spermidine was observed, in agreement with the metabolic analysis. A significant induction in diamino oxidase expression, involved in the catabolism of putrescine, was specifically found in the sensitive genotype ΤΝ6.18, indicating a distinct metabolic response to stress. Present findings highlight the involvement of polyamines in the defense response of <i>Medicago</i> genotypes showing sensitivity to salt stress.https://www.mdpi.com/2223-7747/10/2/269legumesabiotic stresspolyaminesgene expression |
| spellingShingle | Chrystalla Antoniou Xavier Zarza Gholamreza Gohari Sima Panahirad Panagiota Filippou Antonio F. Tiburcio Vasileios Fotopoulos Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress Plants legumes abiotic stress polyamines gene expression |
| title | Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress |
| title_full | Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress |
| title_fullStr | Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress |
| title_full_unstemmed | Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress |
| title_short | Involvement of Polyamine Metabolism in the Response of <i>Medicago truncatula</i> Genotypes to Salt Stress |
| title_sort | involvement of polyamine metabolism in the response of i medicago truncatula i genotypes to salt stress |
| topic | legumes abiotic stress polyamines gene expression |
| url | https://www.mdpi.com/2223-7747/10/2/269 |
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