Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1
Representatives of Subclass Elasmobranchii are cartilaginous fish whose members include sharks, skates, and rays. Because of their unique phylogenetic position of being the most primitive group of vertebrates to possess all the components necessary for an adaptive immune system, the immune regulator...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-08-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/11/9/3224 |
| _version_ | 1828112920312020992 |
|---|---|
| author | Courtney Bennett Ashby B. Bodine Marissa Ciesla Philip Adams Brittany Leigh Stephanie R. Leggett Jodi Miedema Theresa Cantu Carl A. Luer Jennifer E. Yordy Catherine J. Walsh |
| author_facet | Courtney Bennett Ashby B. Bodine Marissa Ciesla Philip Adams Brittany Leigh Stephanie R. Leggett Jodi Miedema Theresa Cantu Carl A. Luer Jennifer E. Yordy Catherine J. Walsh |
| author_sort | Courtney Bennett |
| collection | DOAJ |
| description | Representatives of Subclass Elasmobranchii are cartilaginous fish whose members include sharks, skates, and rays. Because of their unique phylogenetic position of being the most primitive group of vertebrates to possess all the components necessary for an adaptive immune system, the immune regulatory compounds they possess may represent the earliest evolutionary forms of novel compounds with the potential for innovative therapeutic applications. Conditioned medium, generated from short term culture of cells from the epigonal organ of bonnethead sharks (Sphyrna tiburo), has been shown to have potent reproducible cytotoxic activity against a variety of human tumor cell lines in vitro. Existing data suggest that epigonal conditioned medium (ECM) exerts this cytotoxic activity through induction of apoptosis in target cells. This manuscript describes apoptosis induction in a representative tumor cell line, Jurkat E6-1, in response to treatment with ECM at concentrations of 1 and 2 mg/mL. Data indicate that ECM exposure initiates the mitochondrial pathway of apoptosis through activation of caspase enzymes. Future purification of ECM components may result in the isolation of an immune-regulatory compound with potential therapeutic benefit for treatment of human cancer. |
| first_indexed | 2024-04-11T11:58:00Z |
| format | Article |
| id | doaj.art-f284b3bd6ffb469486ff6065f00aa480 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T11:58:00Z |
| publishDate | 2013-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f284b3bd6ffb469486ff6065f00aa4802022-12-22T04:25:05ZengMDPI AGMarine Drugs1660-33972013-08-011193224325710.3390/md11093224Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1Courtney BennettAshby B. BodineMarissa CieslaPhilip AdamsBrittany LeighStephanie R. LeggettJodi MiedemaTheresa CantuCarl A. LuerJennifer E. YordyCatherine J. WalshRepresentatives of Subclass Elasmobranchii are cartilaginous fish whose members include sharks, skates, and rays. Because of their unique phylogenetic position of being the most primitive group of vertebrates to possess all the components necessary for an adaptive immune system, the immune regulatory compounds they possess may represent the earliest evolutionary forms of novel compounds with the potential for innovative therapeutic applications. Conditioned medium, generated from short term culture of cells from the epigonal organ of bonnethead sharks (Sphyrna tiburo), has been shown to have potent reproducible cytotoxic activity against a variety of human tumor cell lines in vitro. Existing data suggest that epigonal conditioned medium (ECM) exerts this cytotoxic activity through induction of apoptosis in target cells. This manuscript describes apoptosis induction in a representative tumor cell line, Jurkat E6-1, in response to treatment with ECM at concentrations of 1 and 2 mg/mL. Data indicate that ECM exposure initiates the mitochondrial pathway of apoptosis through activation of caspase enzymes. Future purification of ECM components may result in the isolation of an immune-regulatory compound with potential therapeutic benefit for treatment of human cancer.http://www.mdpi.com/1660-3397/11/9/3224epigonal organbonnethead sharkJurkatapoptosistumor cell line |
| spellingShingle | Courtney Bennett Ashby B. Bodine Marissa Ciesla Philip Adams Brittany Leigh Stephanie R. Leggett Jodi Miedema Theresa Cantu Carl A. Luer Jennifer E. Yordy Catherine J. Walsh Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 Marine Drugs epigonal organ bonnethead shark Jurkat apoptosis tumor cell line |
| title | Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 |
| title_full | Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 |
| title_fullStr | Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 |
| title_full_unstemmed | Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 |
| title_short | Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1 |
| title_sort | epigonal conditioned media from bonnethead shark sphyrna tiburo induces apoptosis in a t cell leukemia cell line jurkat e6 1 |
| topic | epigonal organ bonnethead shark Jurkat apoptosis tumor cell line |
| url | http://www.mdpi.com/1660-3397/11/9/3224 |
| work_keys_str_mv | AT courtneybennett epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT ashbybbodine epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT marissaciesla epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT philipadams epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT brittanyleigh epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT stephanierleggett epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT jodimiedema epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT theresacantu epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT carlaluer epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT jennifereyordy epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 AT catherinejwalsh epigonalconditionedmediafrombonnetheadsharksphyrnatiburoinducesapoptosisinatcellleukemiacelllinejurkate61 |