A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens
ABSTRACT The objective of this study was to evaluate the effect of eubiotics on the intestinal morphology of broilers. For this purpose, 125 birds were divided into six groups with two replicates each (10 birds in each replicate). Group A was given a Basal diet. All groups except group A were challe...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação APINCO de Ciência e Tecnologia Avícolas
2023-08-01
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| Series: | Brazilian Journal of Poultry Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2023000300306&lng=en&tlng=en |
| _version_ | 1797739653723324416 |
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| author | AR Raffie A Aslam MY Tipu I Altaf A Mustafa MS Imran MZ Farooq G Abbas N Goswami M Mohsin AA Khan S Aslam K Abdul |
| author_facet | AR Raffie A Aslam MY Tipu I Altaf A Mustafa MS Imran MZ Farooq G Abbas N Goswami M Mohsin AA Khan S Aslam K Abdul |
| author_sort | AR Raffie |
| collection | DOAJ |
| description | ABSTRACT The objective of this study was to evaluate the effect of eubiotics on the intestinal morphology of broilers. For this purpose, 125 birds were divided into six groups with two replicates each (10 birds in each replicate). Group A was given a Basal diet. All groups except group A were challenged with Salmonella enterica serovar Enteritidis. Group B was provided the basal diet, group C was fed a Probiotic-added diet; group D was fed a Prebiotics-based diet; group E was given essential oils plus the basal diet; and group F was provided with organic acids plus the basal diet. Two separate experiments were carried out for Salmonella recovery, checking the cecal tonsils and conducting an intestinal pathomorphic evaluation. Villus length, villus width, villus surface area, and crypt depth were measured by micrometry. There was an overall improvement (p<0.05) in intestinal morphometric parameters for all the treatment groups except for the negative control group, which showed the lowest villus height and villus depth values. Maximum villus height (p<0.05) of the duodenum was achieved by group E, which was fed a diet containing essential oils, whereas a maximum villus surface area index (p<0.05) was recorded for the birds of Group D, which were fed a diet containing prebiotics. Maximum villus height (p<0.05) and surface area index in ileum mucosa was recorded (p<0.05) in the birds of group D (treated with prebiotics). It is concluded that there is an overall increase in the gut histology of broilers fed non-antibiotic based feed. |
| first_indexed | 2024-03-12T14:00:18Z |
| format | Article |
| id | doaj.art-f286a722f77e4cef9d719443aa7252c6 |
| institution | Directory Open Access Journal |
| issn | 1806-9061 |
| language | English |
| last_indexed | 2024-03-12T14:00:18Z |
| publishDate | 2023-08-01 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| record_format | Article |
| series | Brazilian Journal of Poultry Science |
| spelling | doaj.art-f286a722f77e4cef9d719443aa7252c62023-08-22T07:41:43ZengFundação APINCO de Ciência e Tecnologia AvícolasBrazilian Journal of Poultry Science1806-90612023-08-0125310.1590/1806-9061-2022-1620A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler ChickensAR Raffiehttps://orcid.org/0000-0003-1585-3850A Aslamhttps://orcid.org/0000-0003-0383-6930MY Tipuhttps://orcid.org/0000-0002-6996-7258I Altafhttps://orcid.org/0000-0002-0157-4239A Mustafahttps://orcid.org/0000-0002-8790-1121MS Imranhttps://orcid.org/0000-0003-4353-6107MZ Farooqhttps://orcid.org/0000-0002-7309-9553G Abbashttps://orcid.org/0000-0002-2741-994XN Goswamihttps://orcid.org/0000-0002-6710-7721M Mohsinhttps://orcid.org/0000-0003-3826-7352AA Khanhttps://orcid.org/0000-0001-8972-8855S Aslamhttps://orcid.org/0009-0002-3712-4616K Abdulhttps://orcid.org/0000-0002-5524-6492ABSTRACT The objective of this study was to evaluate the effect of eubiotics on the intestinal morphology of broilers. For this purpose, 125 birds were divided into six groups with two replicates each (10 birds in each replicate). Group A was given a Basal diet. All groups except group A were challenged with Salmonella enterica serovar Enteritidis. Group B was provided the basal diet, group C was fed a Probiotic-added diet; group D was fed a Prebiotics-based diet; group E was given essential oils plus the basal diet; and group F was provided with organic acids plus the basal diet. Two separate experiments were carried out for Salmonella recovery, checking the cecal tonsils and conducting an intestinal pathomorphic evaluation. Villus length, villus width, villus surface area, and crypt depth were measured by micrometry. There was an overall improvement (p<0.05) in intestinal morphometric parameters for all the treatment groups except for the negative control group, which showed the lowest villus height and villus depth values. Maximum villus height (p<0.05) of the duodenum was achieved by group E, which was fed a diet containing essential oils, whereas a maximum villus surface area index (p<0.05) was recorded for the birds of Group D, which were fed a diet containing prebiotics. Maximum villus height (p<0.05) and surface area index in ileum mucosa was recorded (p<0.05) in the birds of group D (treated with prebiotics). It is concluded that there is an overall increase in the gut histology of broilers fed non-antibiotic based feed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2023000300306&lng=en&tlng=enNon-Antibiotic Feed AdditivesPrebioticsSalmonellaessential oilsorganic acids |
| spellingShingle | AR Raffie A Aslam MY Tipu I Altaf A Mustafa MS Imran MZ Farooq G Abbas N Goswami M Mohsin AA Khan S Aslam K Abdul A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens Brazilian Journal of Poultry Science Non-Antibiotic Feed Additives Prebiotics Salmonella essential oils organic acids |
| title | A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens |
| title_full | A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens |
| title_fullStr | A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens |
| title_full_unstemmed | A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens |
| title_short | A Comparative Study of the Effect of Non-Antibiotic Feed Additives on Experimental Colonization of Salmonella Enterica Serovar Enteridis and Intestinal Pathomorphology in Broiler Chickens |
| title_sort | comparative study of the effect of non antibiotic feed additives on experimental colonization of salmonella enterica serovar enteridis and intestinal pathomorphology in broiler chickens |
| topic | Non-Antibiotic Feed Additives Prebiotics Salmonella essential oils organic acids |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2023000300306&lng=en&tlng=en |
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