Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall.
The herbaceous peony (<i>Paeonia lactiflora</i> Pall.) is widely cultivated as an ornamental, medicinal and edible plant in China. Drought stress can seriously affect the growth of herbaceous peony and reduce its quality. In our previous research, a significantly differentially expressed...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15695 |
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| author | Jiasong Meng Jinhui Guo Tingting Li Zijie Chen Miao Li Daqiu Zhao Jun Tao |
| author_facet | Jiasong Meng Jinhui Guo Tingting Li Zijie Chen Miao Li Daqiu Zhao Jun Tao |
| author_sort | Jiasong Meng |
| collection | DOAJ |
| description | The herbaceous peony (<i>Paeonia lactiflora</i> Pall.) is widely cultivated as an ornamental, medicinal and edible plant in China. Drought stress can seriously affect the growth of herbaceous peony and reduce its quality. In our previous research, a significantly differentially expressed gene, <i>PM19L</i>, was obtained in herbaceous peony under drought stress based on transcriptome analysis, but little is known about its function. In this study, the first <i>PM19L</i> that was isolated in herbaceous peony was comprised of 910 bp, and was designated as <i>PlPM19L</i> (OP480984). It had a complete open reading frame of 537 bp and encoded a 178-amino acid protein with a molecular weight of 18.95 kDa, which was located in the membrane. When <i>PlPM19L</i> was transferred into tobacco, the transgenic plants had enhanced tolerance to drought stress, potentially due to the increase in the abscisic acid (ABA) content and the reduction in the level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In addition, the enhanced ability to scavenge H<sub>2</sub>O<sub>2</sub> under drought stress led to improvements in the enzyme activity and the potential photosynthetic capacity. These results combined suggest that <i>PlPM19L</i> is a key factor to conferring drought stress tolerance in herbaceous peony and provide a scientific theoretical basis for the following improvement in the drought resistance of herbaceous peony and other plants through genetic engineering technology. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:20:15Z |
| publishDate | 2022-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f2872ab1418b409eaa6e7c75290bcb212023-11-24T15:26:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241569510.3390/ijms232415695Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall.Jiasong Meng0Jinhui Guo1Tingting Li2Zijie Chen3Miao Li4Daqiu Zhao5Jun Tao6College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaThe herbaceous peony (<i>Paeonia lactiflora</i> Pall.) is widely cultivated as an ornamental, medicinal and edible plant in China. Drought stress can seriously affect the growth of herbaceous peony and reduce its quality. In our previous research, a significantly differentially expressed gene, <i>PM19L</i>, was obtained in herbaceous peony under drought stress based on transcriptome analysis, but little is known about its function. In this study, the first <i>PM19L</i> that was isolated in herbaceous peony was comprised of 910 bp, and was designated as <i>PlPM19L</i> (OP480984). It had a complete open reading frame of 537 bp and encoded a 178-amino acid protein with a molecular weight of 18.95 kDa, which was located in the membrane. When <i>PlPM19L</i> was transferred into tobacco, the transgenic plants had enhanced tolerance to drought stress, potentially due to the increase in the abscisic acid (ABA) content and the reduction in the level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). In addition, the enhanced ability to scavenge H<sub>2</sub>O<sub>2</sub> under drought stress led to improvements in the enzyme activity and the potential photosynthetic capacity. These results combined suggest that <i>PlPM19L</i> is a key factor to conferring drought stress tolerance in herbaceous peony and provide a scientific theoretical basis for the following improvement in the drought resistance of herbaceous peony and other plants through genetic engineering technology.https://www.mdpi.com/1422-0067/23/24/15695<i>Paeonia lactiflora</i>membrane protein<i>PM19L</i>drought-resistance |
| spellingShingle | Jiasong Meng Jinhui Guo Tingting Li Zijie Chen Miao Li Daqiu Zhao Jun Tao Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. International Journal of Molecular Sciences <i>Paeonia lactiflora</i> membrane protein <i>PM19L</i> drought-resistance |
| title | Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. |
| title_full | Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. |
| title_fullStr | Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. |
| title_full_unstemmed | Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. |
| title_short | Analysis and Functional Verification of <i>PlPM19L</i> Gene Associated with Drought-Resistance in <i>Paeonia lactiflora</i> Pall. |
| title_sort | analysis and functional verification of i plpm19l i gene associated with drought resistance in i paeonia lactiflora i pall |
| topic | <i>Paeonia lactiflora</i> membrane protein <i>PM19L</i> drought-resistance |
| url | https://www.mdpi.com/1422-0067/23/24/15695 |
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