A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice
Investigations into the causative role that western dietary patterns have on obesity and disease pathogenesis have speculated that quality and quantity of dietary fats and/or carbohydrates have a predictive role in the development of these disorders. Standard reference diets such as the AIN-93 roden...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.929446/full |
| _version_ | 1811282727425540096 |
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| author | Sophie B. Chehade George B. H. Green Christopher D. Graham Ayanabha Chakraborti Bijal Vashai Amber Moon Michael B. Williams Benjamin Vickers Taylor Berryhill William Van Der Pol Landon Wilson Mickie L. Powell Daniel L. Smith Stephen Barnes Casey Morrow M. Shahid Mukhtar Gregory D. Kennedy James A. Bibb Stephen A. Watts |
| author_facet | Sophie B. Chehade George B. H. Green Christopher D. Graham Ayanabha Chakraborti Bijal Vashai Amber Moon Michael B. Williams Benjamin Vickers Taylor Berryhill William Van Der Pol Landon Wilson Mickie L. Powell Daniel L. Smith Stephen Barnes Casey Morrow M. Shahid Mukhtar Gregory D. Kennedy James A. Bibb Stephen A. Watts |
| author_sort | Sophie B. Chehade |
| collection | DOAJ |
| description | Investigations into the causative role that western dietary patterns have on obesity and disease pathogenesis have speculated that quality and quantity of dietary fats and/or carbohydrates have a predictive role in the development of these disorders. Standard reference diets such as the AIN-93 rodent diet have historically been used to promote animal health and reduce variation of results across experiments, rather than model modern human dietary habits or nutrition-related pathologies. In rodents high-fat diets (HFDs) became a classic tool to investigate diet-induced obesity (DIO). These murine diets often relied on a single fat source with the most DIO consistent HFDs containing levels of fat up to 45-60% (kcal), higher than the reported human intake of 33–35% (kcal). More recently, researchers are formulating experimental animal (pre-clinical) diets that reflect mean human macro- and micronutrient consumption levels described by the National Health and Nutrition Examination Survey (NHANES). These diets attempt to integrate relevant ingredient sources and levels of nutrients; however, they most often fail to include high-fructose corn syrup (HFCS) as a source of dietary carbohydrate. We have formulated a modified Standard American Diet (mSAD) that incorporates relevant levels and sources of nutrient classes, including dietary HFCS, to assess the basal physiologies associated with mSAD consumption. Mice proffered the mSAD for 15 weeks displayed a phenotype consistent with metabolic syndrome, exhibiting increased adiposity, fasting hyperglycemia with impaired glucose and insulin tolerance. Metabolic alterations were evidenced at the tissue level as crown-like structures (CLS) in adipose tissue and fatty acid deposition in the liver, and targeted 16S rRNA metagenomics revealed microbial compositional shifts between dietary groups. This study suggests diet quality significantly affects metabolic homeostasis, emphasizing the importance of developing relevant pre-clinical diets to investigate chronic diseases highly impacted by western dietary consumption patterns. |
| first_indexed | 2024-04-13T01:57:45Z |
| format | Article |
| id | doaj.art-f28dd96b83cf4163abbaf4e7323f5ea0 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T01:57:45Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-f28dd96b83cf4163abbaf4e7323f5ea02022-12-22T03:07:43ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.929446929446A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J miceSophie B. Chehade0George B. H. Green1Christopher D. Graham2Ayanabha Chakraborti3Bijal Vashai4Amber Moon5Michael B. Williams6Benjamin Vickers7Taylor Berryhill8William Van Der Pol9Landon Wilson10Mickie L. Powell11Daniel L. Smith12Stephen Barnes13Casey Morrow14M. Shahid Mukhtar15Gregory D. Kennedy16James A. Bibb17Stephen A. Watts18Department of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesComprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, United StatesCenter for Clinical and Translational Science, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Nutrition Sciences, Nutrition Obesity Research Center, University of Alabama at Birmingham, Birmingham, AL, United StatesComprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, United StatesDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL, United StatesInvestigations into the causative role that western dietary patterns have on obesity and disease pathogenesis have speculated that quality and quantity of dietary fats and/or carbohydrates have a predictive role in the development of these disorders. Standard reference diets such as the AIN-93 rodent diet have historically been used to promote animal health and reduce variation of results across experiments, rather than model modern human dietary habits or nutrition-related pathologies. In rodents high-fat diets (HFDs) became a classic tool to investigate diet-induced obesity (DIO). These murine diets often relied on a single fat source with the most DIO consistent HFDs containing levels of fat up to 45-60% (kcal), higher than the reported human intake of 33–35% (kcal). More recently, researchers are formulating experimental animal (pre-clinical) diets that reflect mean human macro- and micronutrient consumption levels described by the National Health and Nutrition Examination Survey (NHANES). These diets attempt to integrate relevant ingredient sources and levels of nutrients; however, they most often fail to include high-fructose corn syrup (HFCS) as a source of dietary carbohydrate. We have formulated a modified Standard American Diet (mSAD) that incorporates relevant levels and sources of nutrient classes, including dietary HFCS, to assess the basal physiologies associated with mSAD consumption. Mice proffered the mSAD for 15 weeks displayed a phenotype consistent with metabolic syndrome, exhibiting increased adiposity, fasting hyperglycemia with impaired glucose and insulin tolerance. Metabolic alterations were evidenced at the tissue level as crown-like structures (CLS) in adipose tissue and fatty acid deposition in the liver, and targeted 16S rRNA metagenomics revealed microbial compositional shifts between dietary groups. This study suggests diet quality significantly affects metabolic homeostasis, emphasizing the importance of developing relevant pre-clinical diets to investigate chronic diseases highly impacted by western dietary consumption patterns.https://www.frontiersin.org/articles/10.3389/fnut.2022.929446/fullobesitymetabolic syndromeWestern Diethigh fructose corn syruphigh fat diet |
| spellingShingle | Sophie B. Chehade George B. H. Green Christopher D. Graham Ayanabha Chakraborti Bijal Vashai Amber Moon Michael B. Williams Benjamin Vickers Taylor Berryhill William Van Der Pol Landon Wilson Mickie L. Powell Daniel L. Smith Stephen Barnes Casey Morrow M. Shahid Mukhtar Gregory D. Kennedy James A. Bibb Stephen A. Watts A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice Frontiers in Nutrition obesity metabolic syndrome Western Diet high fructose corn syrup high fat diet |
| title | A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice |
| title_full | A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice |
| title_fullStr | A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice |
| title_full_unstemmed | A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice |
| title_short | A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice |
| title_sort | modified standard american diet induces physiological parameters associated with metabolic syndrome in c57bl 6j mice |
| topic | obesity metabolic syndrome Western Diet high fructose corn syrup high fat diet |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.929446/full |
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