Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy
Abstract The most prevalent primary glomerulonephritis and leading cause of end-stage renal disease worldwide is IgA nephropathy (IgAN). More and more studies are describing urinary microRNA (miRNA) as a non-invasive marker for a variety of renal diseases. We screened candidate miRNAs based on data...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32910-z |
| _version_ | 1827966294735978496 |
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| author | Meng Zhang Zhi-Yu Duan Qiu-Yue Zhang Xie-Guan-Xuan Xu Yan Zhang Peng Wang Shu-Wei Duan Jie Wu Xiang-Mei Chen Guang-Yan Cai |
| author_facet | Meng Zhang Zhi-Yu Duan Qiu-Yue Zhang Xie-Guan-Xuan Xu Yan Zhang Peng Wang Shu-Wei Duan Jie Wu Xiang-Mei Chen Guang-Yan Cai |
| author_sort | Meng Zhang |
| collection | DOAJ |
| description | Abstract The most prevalent primary glomerulonephritis and leading cause of end-stage renal disease worldwide is IgA nephropathy (IgAN). More and more studies are describing urinary microRNA (miRNA) as a non-invasive marker for a variety of renal diseases. We screened candidate miRNAs based on data from three published IgAN urinary sediment miRNAs chips. In separate confirmation and validation cohorts, we included 174 IgAN patients, 100 patients with other nephropathies as disease controls (DC), and 97 normal controls (NC) for quantitative real-time PCR. A total of three candidate miRNAs, miR-16-5p, Let-7g-5p, miR-15a-5p were obtained. In both the confirmation and validation cohorts, these miRNAs levels were considerably higher in the IgAN than in NC, with miR-16-5p significantly higher than in DC. The area under the ROC curve for urinary miR-16-5p levels was 0.73. Correlation analysis suggested that miR-16-5p was positively correlated with endocapillary hypercellularity (r = 0.164 p = 0.031). When miR-16-5p was combined with eGFR, proteinuria and C4, the AUC value for predicting endocapillary hypercellularity was 0.726. By following the renal function of patients with IgAN, the levels of miR-16-5p were noticeably higher in the IgAN progressors than in the non- progressors (p = 0.036). Urinary sediment miR-16-5p can be used as noninvasive biomarkers for the assessment of endocapillary hypercellularity and diagnosis of IgA nephropathy. Furthermore, urinary miR-16-5p may be predictors of renal progression. |
| first_indexed | 2024-04-09T17:47:44Z |
| format | Article |
| id | doaj.art-f29a1563fdd241d3b79fe8baa6e09487 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T17:47:44Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f29a1563fdd241d3b79fe8baa6e094872023-04-16T11:15:27ZengNature PortfolioScientific Reports2045-23222023-04-011311810.1038/s41598-023-32910-zUrinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathyMeng Zhang0Zhi-Yu Duan1Qiu-Yue Zhang2Xie-Guan-Xuan Xu3Yan Zhang4Peng Wang5Shu-Wei Duan6Jie Wu7Xiang-Mei Chen8Guang-Yan Cai9Medical School of Chinese PLAMedical School of Chinese PLAMedical School of Chinese PLAMedical School of Chinese PLADepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchDepartment of Nephrology, First Medical Center of Chinese PLA General Hospital, Nephrology Institute of the Chinese People’s Liberation Army, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Disease ResearchAbstract The most prevalent primary glomerulonephritis and leading cause of end-stage renal disease worldwide is IgA nephropathy (IgAN). More and more studies are describing urinary microRNA (miRNA) as a non-invasive marker for a variety of renal diseases. We screened candidate miRNAs based on data from three published IgAN urinary sediment miRNAs chips. In separate confirmation and validation cohorts, we included 174 IgAN patients, 100 patients with other nephropathies as disease controls (DC), and 97 normal controls (NC) for quantitative real-time PCR. A total of three candidate miRNAs, miR-16-5p, Let-7g-5p, miR-15a-5p were obtained. In both the confirmation and validation cohorts, these miRNAs levels were considerably higher in the IgAN than in NC, with miR-16-5p significantly higher than in DC. The area under the ROC curve for urinary miR-16-5p levels was 0.73. Correlation analysis suggested that miR-16-5p was positively correlated with endocapillary hypercellularity (r = 0.164 p = 0.031). When miR-16-5p was combined with eGFR, proteinuria and C4, the AUC value for predicting endocapillary hypercellularity was 0.726. By following the renal function of patients with IgAN, the levels of miR-16-5p were noticeably higher in the IgAN progressors than in the non- progressors (p = 0.036). Urinary sediment miR-16-5p can be used as noninvasive biomarkers for the assessment of endocapillary hypercellularity and diagnosis of IgA nephropathy. Furthermore, urinary miR-16-5p may be predictors of renal progression.https://doi.org/10.1038/s41598-023-32910-z |
| spellingShingle | Meng Zhang Zhi-Yu Duan Qiu-Yue Zhang Xie-Guan-Xuan Xu Yan Zhang Peng Wang Shu-Wei Duan Jie Wu Xiang-Mei Chen Guang-Yan Cai Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy Scientific Reports |
| title | Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy |
| title_full | Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy |
| title_fullStr | Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy |
| title_full_unstemmed | Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy |
| title_short | Urinary miR-16-5p can be used as a potential marker of endocapillary hypercellularity in IgA nephropathy |
| title_sort | urinary mir 16 5p can be used as a potential marker of endocapillary hypercellularity in iga nephropathy |
| url | https://doi.org/10.1038/s41598-023-32910-z |
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