Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing
Citrus huanglongbing (HLB) is a destructive disease caused by <i>Candidatus</i> Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, <i>Bacillus amyloliquefaciens</i> G...
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MDPI AG
2021-01-01
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/261 |
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| author | Jing Nan Shaoran Zhang Ling Jiang |
| author_facet | Jing Nan Shaoran Zhang Ling Jiang |
| author_sort | Jing Nan |
| collection | DOAJ |
| description | Citrus huanglongbing (HLB) is a destructive disease caused by <i>Candidatus</i> Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, <i>Bacillus amyloliquefaciens</i> GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by <i>Ca.</i> L. asiaticus were treated with <i>B. amyloliquefaciens</i> GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with <i>B. amyloliquefaciens</i> GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with <i>B. amyloliquefaciens</i> GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of <i>B. amyloliquefaciens</i> GJ1 by high-performance liquid chromatography. These results indicate that <i>B. amyloliquefaciens</i> GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes. |
| first_indexed | 2024-03-09T03:19:55Z |
| format | Article |
| id | doaj.art-f29cca52589647858f1a02a0c9e8cba7 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:19:55Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f29cca52589647858f1a02a0c9e8cba72023-12-03T15:14:25ZengMDPI AGPlants2223-77472021-01-0110226110.3390/plants10020261Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus HuanglongbingJing Nan0Shaoran Zhang1Ling Jiang2College of Horticulture and Forestry, Ministry of Education Key Laboratory of Plant Biology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry, Ministry of Education Key Laboratory of Plant Biology, Huazhong Agricultural University, Wuhan 430070, ChinaCitrus huanglongbing (HLB) is a destructive disease caused by <i>Candidatus</i> Liberibacter species and is a serious global concern for the citrus industry. To date, there is no established strategy for control of this disease. Previously, <i>Bacillus amyloliquefaciens</i> GJ1 was screened as the biocontrol agent against HLB. In this study, two-year-old citrus infected by <i>Ca.</i> L. asiaticus were treated with <i>B. amyloliquefaciens</i> GJ1 solution via root irrigation. In these plants, after seven irrigation treatments, the results indicated that the photosynthetic parameters, chlorophyll content, resistance-associated enzyme content and the expression of defense-related genes were significantly higher than for the plants treated with the same volume water. The content of starch and soluble sugar were significantly lower, compared to the control treatment. The parallel reaction monitoring (PRM) results revealed that treatment with <i>B. amyloliquefaciens</i> GJ1 solution, the expression levels of 3 proteins with photosynthetic function were upregulated in citrus leaves. The accumulation of reactive oxygen species (ROS) in citrus leaves treated with <i>B. amyloliquefaciens</i> GJ1 flag22 was significantly higher than untreated plants and induced the defense-related gene expression in citrus. Finally, surfactin was identified from the fermentation broth of <i>B. amyloliquefaciens</i> GJ1 by high-performance liquid chromatography. These results indicate that <i>B. amyloliquefaciens</i> GJ1 may improve the immunity of citrus by increasing the photosynthesis and enhancing the expression of the resistance-related genes.https://www.mdpi.com/2223-7747/10/2/261huanglongbing<i>Bacillus amyloliquefaciens</i> GJ1biocontrolinduced systemic resistance |
| spellingShingle | Jing Nan Shaoran Zhang Ling Jiang Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing Plants huanglongbing <i>Bacillus amyloliquefaciens</i> GJ1 biocontrol induced systemic resistance |
| title | Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing |
| title_full | Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing |
| title_fullStr | Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing |
| title_full_unstemmed | Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing |
| title_short | Antibacterial Potential of <i>Bacillus amyloliquefaciens</i> GJ1 against Citrus Huanglongbing |
| title_sort | antibacterial potential of i bacillus amyloliquefaciens i gj1 against citrus huanglongbing |
| topic | huanglongbing <i>Bacillus amyloliquefaciens</i> GJ1 biocontrol induced systemic resistance |
| url | https://www.mdpi.com/2223-7747/10/2/261 |
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