Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer
Abstract Mounting evidence indicate that cuproptosis, a novel form of programmed cell death, contributes to cancer development and progression. However, a comprehensive analysis regarding the expressions, functions, and regulatory network of cuproptosis-related genes is still lacking. In the present...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45761-5 |
| _version_ | 1827781184568950784 |
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| author | Jia-hao Wu Tian-cheng Cheng Bei Zhu Hai-yan Gao Lin Zheng Wei-xian Chen |
| author_facet | Jia-hao Wu Tian-cheng Cheng Bei Zhu Hai-yan Gao Lin Zheng Wei-xian Chen |
| author_sort | Jia-hao Wu |
| collection | DOAJ |
| description | Abstract Mounting evidence indicate that cuproptosis, a novel form of programmed cell death, contributes to cancer development and progression. However, a comprehensive analysis regarding the expressions, functions, and regulatory network of cuproptosis-related genes is still lacking. In the present work, cuproptosis-related genes, upstream miRNAs and lncRNAs, and clinical data of breast cancer from TCGA database were analyzed by R language including Cox regression analysis, correlation calculation, ROC curve construction, and survival evaluation, and were further verified by public-available databases. Chemosensitivity and immune infiltration were also evaluated by online tools. SLC31A1 was significantly increased in breast cancer samples than those in normal tissues. SLC31A1 was negatively related to a favorable outcome in breast cancer, and the AUC value increased with the prolongation of follow-up time. LINC01614 and miR-204-5p were potential upstream regulators of SLC31A1. Moreover, SLC31A1 was significantly positively correlated with different immune cells infiltration, immune cell biomarkers, and immune checkpoints in breast cancer. SLC31A1 was a potential cuproptosis-related gene in breast cancer, which was significantly upregulated and was able to predict diagnosis, prognosis, chemosensitivity, and immune infiltration. LINC01640/miR-204-5p/SLC31A1 might be a significant and promising axis during cuproptosis in breast cancer. |
| first_indexed | 2024-03-11T15:15:17Z |
| format | Article |
| id | doaj.art-f29d31d0b5a0414f8230527ae02380ca |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T15:15:17Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f29d31d0b5a0414f8230527ae02380ca2023-10-29T12:23:59ZengNature PortfolioScientific Reports2045-23222023-10-0113111510.1038/s41598-023-45761-5Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancerJia-hao Wu0Tian-cheng Cheng1Bei Zhu2Hai-yan Gao3Lin Zheng4Wei-xian Chen5Department of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityDepartment of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityDepartment of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityDepartment of Breast Surgery, The Affiliated Changzhou Tumor Hospital of Soochow UniversityDepartment of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityDepartment of Breast Surgery, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityAbstract Mounting evidence indicate that cuproptosis, a novel form of programmed cell death, contributes to cancer development and progression. However, a comprehensive analysis regarding the expressions, functions, and regulatory network of cuproptosis-related genes is still lacking. In the present work, cuproptosis-related genes, upstream miRNAs and lncRNAs, and clinical data of breast cancer from TCGA database were analyzed by R language including Cox regression analysis, correlation calculation, ROC curve construction, and survival evaluation, and were further verified by public-available databases. Chemosensitivity and immune infiltration were also evaluated by online tools. SLC31A1 was significantly increased in breast cancer samples than those in normal tissues. SLC31A1 was negatively related to a favorable outcome in breast cancer, and the AUC value increased with the prolongation of follow-up time. LINC01614 and miR-204-5p were potential upstream regulators of SLC31A1. Moreover, SLC31A1 was significantly positively correlated with different immune cells infiltration, immune cell biomarkers, and immune checkpoints in breast cancer. SLC31A1 was a potential cuproptosis-related gene in breast cancer, which was significantly upregulated and was able to predict diagnosis, prognosis, chemosensitivity, and immune infiltration. LINC01640/miR-204-5p/SLC31A1 might be a significant and promising axis during cuproptosis in breast cancer.https://doi.org/10.1038/s41598-023-45761-5 |
| spellingShingle | Jia-hao Wu Tian-cheng Cheng Bei Zhu Hai-yan Gao Lin Zheng Wei-xian Chen Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer Scientific Reports |
| title | Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer |
| title_full | Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer |
| title_fullStr | Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer |
| title_full_unstemmed | Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer |
| title_short | Identification of cuproptosis-related gene SLC31A1 and upstream LncRNA-miRNA regulatory axis in breast cancer |
| title_sort | identification of cuproptosis related gene slc31a1 and upstream lncrna mirna regulatory axis in breast cancer |
| url | https://doi.org/10.1038/s41598-023-45761-5 |
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