Challenges in Direct Detection of Flaviviruses: A Review
Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of e...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/5/643 |
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| author | Bruna de Paula Dias Camila Cavadas Barbosa Cyntia Silva Ferreira Samara Mayra Soares Alves dos Santos Orlando Alfredo Pineda Arrieta Wellington Carvalho Malta Maria Laura Maximiano Dias Gomes Mariela Alves e Silva Júlia de Matos Fonseca Lysandro Pinto Borges Breno de Mello Silva |
| author_facet | Bruna de Paula Dias Camila Cavadas Barbosa Cyntia Silva Ferreira Samara Mayra Soares Alves dos Santos Orlando Alfredo Pineda Arrieta Wellington Carvalho Malta Maria Laura Maximiano Dias Gomes Mariela Alves e Silva Júlia de Matos Fonseca Lysandro Pinto Borges Breno de Mello Silva |
| author_sort | Bruna de Paula Dias |
| collection | DOAJ |
| description | Arthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology. |
| first_indexed | 2024-03-11T03:25:58Z |
| format | Article |
| id | doaj.art-f29f68f8984d470d81871b42cff23c0e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T03:25:58Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-f29f68f8984d470d81871b42cff23c0e2023-11-18T02:46:18ZengMDPI AGPathogens2076-08172023-04-0112564310.3390/pathogens12050643Challenges in Direct Detection of Flaviviruses: A ReviewBruna de Paula Dias0Camila Cavadas Barbosa1Cyntia Silva Ferreira2Samara Mayra Soares Alves dos Santos3Orlando Alfredo Pineda Arrieta4Wellington Carvalho Malta5Maria Laura Maximiano Dias Gomes6Mariela Alves e Silva7Júlia de Matos Fonseca8Lysandro Pinto Borges9Breno de Mello Silva10Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilDepartment of Pharmacy, Federal University of Sergipe, São Cristóvão 9100-000, BrazilDepartment of Biological Sciences, Federal University of Ouro Preto, Ouro Preto 35400-000, BrazilArthropods transmit arboviruses via mosquito and tick bites to humans and other animals. The genus flavivirus, which causes diseases, sequelae, and thousands of deaths, mainly in developing and underdeveloped countries, is among the arboviruses of interest to public health. Given the importance of early and accurate diagnosis, this review analyzes the methods of direct detection of flaviviruses, such as reverse transcription loop-mediated isothermal amplification, microfluidics, localized surface plasmon resonance, and surface-enhanced Raman scattering, and presents the advantages, disadvantages, and detection limits identified in studies reported in the literature for each methodology. Among the different methods available, it is essential to balance four fundamental indicators to determine the ideal test: good sensitivity, high specificity, low false positive rate, and rapid results. Among the methods analyzed, reverse transcription loop-mediated isothermal amplification stands out, owing to result availability within a few minutes, with good sensitivity and specificity; in addition, it is the best-characterized methodology.https://www.mdpi.com/2076-0817/12/5/643flavivirusesarbovirusesdiagnosisdirect detection |
| spellingShingle | Bruna de Paula Dias Camila Cavadas Barbosa Cyntia Silva Ferreira Samara Mayra Soares Alves dos Santos Orlando Alfredo Pineda Arrieta Wellington Carvalho Malta Maria Laura Maximiano Dias Gomes Mariela Alves e Silva Júlia de Matos Fonseca Lysandro Pinto Borges Breno de Mello Silva Challenges in Direct Detection of Flaviviruses: A Review Pathogens flaviviruses arboviruses diagnosis direct detection |
| title | Challenges in Direct Detection of Flaviviruses: A Review |
| title_full | Challenges in Direct Detection of Flaviviruses: A Review |
| title_fullStr | Challenges in Direct Detection of Flaviviruses: A Review |
| title_full_unstemmed | Challenges in Direct Detection of Flaviviruses: A Review |
| title_short | Challenges in Direct Detection of Flaviviruses: A Review |
| title_sort | challenges in direct detection of flaviviruses a review |
| topic | flaviviruses arboviruses diagnosis direct detection |
| url | https://www.mdpi.com/2076-0817/12/5/643 |
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