Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells
Endothelial dysfunction is closely relevant to atherosclerosis complications in diabetic patients. Hispidulin, a flavone derived from the herb Salvia plebeia R. Br., has numerous biological properties including anti-inflammatory and antioxidative effects, but the underlying mechanism of its anti-inf...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616302997 |
| _version_ | 1818596364878086144 |
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| author | Weiwei Qin Junxiao Xi Baiqiu He Baobao Zhang Huiling Luan Feihua Wu |
| author_facet | Weiwei Qin Junxiao Xi Baiqiu He Baobao Zhang Huiling Luan Feihua Wu |
| author_sort | Weiwei Qin |
| collection | DOAJ |
| description | Endothelial dysfunction is closely relevant to atherosclerosis complications in diabetic patients. Hispidulin, a flavone derived from the herb Salvia plebeia R. Br., has numerous biological properties including anti-inflammatory and antioxidative effects, but the underlying mechanism of its anti-inflammatory action remains unclear. This study was designed to investigate the effects of hispidulin on endothelial homeostasis and its mechanism. Hispidulin effectively inhibited high glucose-induced oxidative stress by attenuating PKCβII phosphorylation and downstream reactive oxygen species (ROS) production, furthermore reversing the loss of mitochondria membrane potential. Moreover, hispidulin significantly suppressed the expression of NLRP3 inflammasome and IKKβ/NF-κB, and restored high glucose-impaired vasodilation in rat aorta. This study demonstrated that hispidulin ameliorated high glucose-mediated endothelial dysfunction via inhibiting PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling. Besides, these findings indicate the beneficial effects of hispidulin on the improvement of endothelial dysfunction and explain its potential application in the prevention and treatment of diabetic vascular complications. |
| first_indexed | 2024-12-16T11:30:45Z |
| format | Article |
| id | doaj.art-f2a49cc6c7ff43bcb15196f059e838df |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T11:30:45Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-f2a49cc6c7ff43bcb15196f059e838df2022-12-21T22:33:15ZengElsevierJournal of Functional Foods1756-46462016-12-0127392405Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cellsWeiwei Qin0Junxiao Xi1Baiqiu He2Baobao Zhang3Huiling Luan4Feihua Wu5Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, ChinaDepartment of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China; Corresponding author. Department of Pharmacology of Chinese Materia Medica and Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, 639 Longmian Avenue, Nanjing 211198, China.Endothelial dysfunction is closely relevant to atherosclerosis complications in diabetic patients. Hispidulin, a flavone derived from the herb Salvia plebeia R. Br., has numerous biological properties including anti-inflammatory and antioxidative effects, but the underlying mechanism of its anti-inflammatory action remains unclear. This study was designed to investigate the effects of hispidulin on endothelial homeostasis and its mechanism. Hispidulin effectively inhibited high glucose-induced oxidative stress by attenuating PKCβII phosphorylation and downstream reactive oxygen species (ROS) production, furthermore reversing the loss of mitochondria membrane potential. Moreover, hispidulin significantly suppressed the expression of NLRP3 inflammasome and IKKβ/NF-κB, and restored high glucose-impaired vasodilation in rat aorta. This study demonstrated that hispidulin ameliorated high glucose-mediated endothelial dysfunction via inhibiting PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling. Besides, these findings indicate the beneficial effects of hispidulin on the improvement of endothelial dysfunction and explain its potential application in the prevention and treatment of diabetic vascular complications.http://www.sciencedirect.com/science/article/pii/S1756464616302997HispidulinEndothelial dysfunctionPKCβIINLRP3 inflammasomeOxidative stressHigh glucose |
| spellingShingle | Weiwei Qin Junxiao Xi Baiqiu He Baobao Zhang Huiling Luan Feihua Wu Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells Journal of Functional Foods Hispidulin Endothelial dysfunction PKCβII NLRP3 inflammasome Oxidative stress High glucose |
| title | Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells |
| title_full | Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells |
| title_fullStr | Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells |
| title_full_unstemmed | Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells |
| title_short | Ameliorative effects of hispidulin on high glucose-mediated endothelial dysfunction via inhibition of PKCβII-associated NLRP3 inflammasome activation and NF-κB signaling in endothelial cells |
| title_sort | ameliorative effects of hispidulin on high glucose mediated endothelial dysfunction via inhibition of pkcβii associated nlrp3 inflammasome activation and nf κb signaling in endothelial cells |
| topic | Hispidulin Endothelial dysfunction PKCβII NLRP3 inflammasome Oxidative stress High glucose |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616302997 |
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