Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-10-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | http://www.journalofextracellularvesicles.net/index.php/jev/article/view/32945/pdf_75 |
| _version_ | 1818085648806969344 |
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| author | Chris Gardiner Dolores Di Vizio Susmita Sahoo Clotilde Théry Kenneth W. Witwer Marca Wauben Andrew F. Hill |
| author_facet | Chris Gardiner Dolores Di Vizio Susmita Sahoo Clotilde Théry Kenneth W. Witwer Marca Wauben Andrew F. Hill |
| author_sort | Chris Gardiner |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed. |
| first_indexed | 2024-12-10T20:13:08Z |
| format | Article |
| id | doaj.art-f2a857be7d0a41bab6642985c7f9568a |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-12-10T20:13:08Z |
| publishDate | 2016-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-f2a857be7d0a41bab6642985c7f9568a2022-12-22T01:35:15ZengWileyJournal of Extracellular Vesicles2001-30782016-10-01501610.3402/jev.v5.3294532945Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide surveyChris Gardiner0Dolores Di Vizio1Susmita Sahoo2Clotilde Théry3Kenneth W. Witwer4Marca Wauben5Andrew F. Hill6 Haemostasis Research Unit, Research Department of Haematology, University College London, London, UK Icahn School of Medicine, Cardiovascular Research Center, Cedars-Sinai, Los Angeles, CA, USA Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA Institut Curie, PSL Research University, INSERM U932, Paris, France Johns Hopkins University School of Medicine, Baltimore, USA Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, AustraliaExtracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.http://www.journalofextracellularvesicles.net/index.php/jev/article/view/32945/pdf_75extracellular vesiclesisolationcharacterizationpurificationflow cytometryproteomicsRNA analysissingle vesicle analysis |
| spellingShingle | Chris Gardiner Dolores Di Vizio Susmita Sahoo Clotilde Théry Kenneth W. Witwer Marca Wauben Andrew F. Hill Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey Journal of Extracellular Vesicles extracellular vesicles isolation characterization purification flow cytometry proteomics RNA analysis single vesicle analysis |
| title | Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey |
| title_full | Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey |
| title_fullStr | Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey |
| title_full_unstemmed | Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey |
| title_short | Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey |
| title_sort | techniques used for the isolation and characterization of extracellular vesicles results of a worldwide survey |
| topic | extracellular vesicles isolation characterization purification flow cytometry proteomics RNA analysis single vesicle analysis |
| url | http://www.journalofextracellularvesicles.net/index.php/jev/article/view/32945/pdf_75 |
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