Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection
Leaf spot disease caused by <i>Alternaria</i> pathogens seriously threatens peony production. The physiological mechanism of peony resistance to the pathogen is little reported. This study aimed to reveal the defensive mechanism of peonies in response to the pathogen <i>Alternaria ...
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MDPI AG
2023-07-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/8/862 |
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| author | Yang Wu Zhonghua Lu Yongfang Zhu Xianfeng Guo |
| author_facet | Yang Wu Zhonghua Lu Yongfang Zhu Xianfeng Guo |
| author_sort | Yang Wu |
| collection | DOAJ |
| description | Leaf spot disease caused by <i>Alternaria</i> pathogens seriously threatens peony production. The physiological mechanism of peony resistance to the pathogen is little reported. This study aimed to reveal the defensive mechanism of peonies in response to the pathogen <i>Alternaria tenuissima</i>. The disease-resistant (R) variety ‘Zi Fengyu’ and susceptible (S) variety ‘Heihai Botao’ were employed, and some parameters in the leaves were analyzed after inoculation with <i>A. tenuissima</i>, mainly including the hypersensitive response (HR), activity of defensive enzymes, and expression of disease-resistance genes. The results showed that compared with the responses in the S genotype, HR occurred more rapidly in the R genotype. Meanwhile, the activity of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) and other two defense enzymes (polyphenol oxidase and phenylalanine ammonia-lyase) increased more significantly, and the expression of <i>pathogenesis-related</i> (<i>PR</i>) genes (<i>PlPR1</i>, <i>PlPR2</i>, <i>PlPR4B</i>, <i>PlPR5</i>, and <i>PlPR10</i>) and two <i>WRKY</i> genes (<i>PlWRKY13</i> and <i>PlWRKY65</i>) was more strongly induced. These responses collectively contributed to the disease resistance of the R genotype. These findings provided a theoretical basis for understanding the intrinsic mechanism of peony resistance to <i>Alternaria</i> leaf spot disease and breeding the disease-resistant peony varieties using a molecular approach. |
| first_indexed | 2024-03-10T23:54:52Z |
| format | Article |
| id | doaj.art-f2aaee2ce7bd48cca1865fbfa3c58606 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:54:52Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-f2aaee2ce7bd48cca1865fbfa3c586062023-11-19T01:20:49ZengMDPI AGHorticulturae2311-75242023-07-019886210.3390/horticulturae9080862Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> InfectionYang Wu0Zhonghua Lu1Yongfang Zhu2Xianfeng Guo3College of Forestry, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Forestry, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Forestry, Shandong Agricultural University, Tai’an 271018, ChinaCollege of Forestry, Shandong Agricultural University, Tai’an 271018, ChinaLeaf spot disease caused by <i>Alternaria</i> pathogens seriously threatens peony production. The physiological mechanism of peony resistance to the pathogen is little reported. This study aimed to reveal the defensive mechanism of peonies in response to the pathogen <i>Alternaria tenuissima</i>. The disease-resistant (R) variety ‘Zi Fengyu’ and susceptible (S) variety ‘Heihai Botao’ were employed, and some parameters in the leaves were analyzed after inoculation with <i>A. tenuissima</i>, mainly including the hypersensitive response (HR), activity of defensive enzymes, and expression of disease-resistance genes. The results showed that compared with the responses in the S genotype, HR occurred more rapidly in the R genotype. Meanwhile, the activity of antioxidant enzymes (superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase) and other two defense enzymes (polyphenol oxidase and phenylalanine ammonia-lyase) increased more significantly, and the expression of <i>pathogenesis-related</i> (<i>PR</i>) genes (<i>PlPR1</i>, <i>PlPR2</i>, <i>PlPR4B</i>, <i>PlPR5</i>, and <i>PlPR10</i>) and two <i>WRKY</i> genes (<i>PlWRKY13</i> and <i>PlWRKY65</i>) was more strongly induced. These responses collectively contributed to the disease resistance of the R genotype. These findings provided a theoretical basis for understanding the intrinsic mechanism of peony resistance to <i>Alternaria</i> leaf spot disease and breeding the disease-resistant peony varieties using a molecular approach.https://www.mdpi.com/2311-7524/9/8/862activity of defensive enzymes<i>Alternaria tenuissima</i>gene expressionherbaceous peonyleaf spot disease |
| spellingShingle | Yang Wu Zhonghua Lu Yongfang Zhu Xianfeng Guo Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection Horticulturae activity of defensive enzymes <i>Alternaria tenuissima</i> gene expression herbaceous peony leaf spot disease |
| title | Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection |
| title_full | Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection |
| title_fullStr | Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection |
| title_full_unstemmed | Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection |
| title_short | Physiological and Gene Expression Analysis of Herbaceous Peony Resistance to <i>Alternaria tenuissima</i> Infection |
| title_sort | physiological and gene expression analysis of herbaceous peony resistance to i alternaria tenuissima i infection |
| topic | activity of defensive enzymes <i>Alternaria tenuissima</i> gene expression herbaceous peony leaf spot disease |
| url | https://www.mdpi.com/2311-7524/9/8/862 |
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