Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer
Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/3/179 |
| _version_ | 1818035565619052544 |
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| author | Danae Jiménez-Cárcamo Carlos García Héctor R. Contreras |
| author_facet | Danae Jiménez-Cárcamo Carlos García Héctor R. Contreras |
| author_sort | Danae Jiménez-Cárcamo |
| collection | DOAJ |
| description | Diarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhibitors, mainly type 1 protein phosphatase (PP1) and type 2A protein phosphatase (PP2A). Different effects have been reported at the cellular, molecular and genetic levels. In this study, changes in cell survival and cell mobility induced by OA, DTX-1 and DTX-2 were determined in epithelial cell lines of the colon and colon cancer. The cell viability results showed that tumoral cell lines were more resistant to toxins than the nontumoral cell line. The results of the functional assays for testing cell migration, evaluation of cell death and the expression of proteins associated with cell adhesion showed a dual effect of toxins since in the nontumoral cell line, a greater induction of cell death, presumably by anoikis, was detected. In the tumoral cell lines, there was an induction of a more aggressive phenotype characterized by increased resistance to toxins, increased migration and increased FAK activation. In tumoral cell lines of colon cancer, OA, DTX-1/DTX-2 induce a more aggressive phenotype. |
| first_indexed | 2024-12-10T06:57:05Z |
| format | Article |
| id | doaj.art-f2ac04e7ddab40be86a669608b4280aa |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-12-10T06:57:05Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f2ac04e7ddab40be86a669608b4280aa2022-12-22T01:58:25ZengMDPI AGToxins2072-66512020-03-0112317910.3390/toxins12030179toxins12030179Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon CancerDanae Jiménez-Cárcamo0Carlos García1Héctor R. Contreras2Department of Basic and Clinical Oncology, Faculty of Medicine, University of Chile, Santiago 8380453, ChilePhysiology and Biophysics Program, ICBM, Faculty of Medicine, University of Chile, Santiago 8380453, ChileDepartment of Basic and Clinical Oncology, Faculty of Medicine, University of Chile, Santiago 8380453, ChileDiarrhetic shellfish poisoning (DSP) is a syndrome caused by the intake of shellfish contaminated with a group of lipophilic and thermostable toxins, which consists of okadaic acid (OA), dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2). These toxins are potent protein Ser/Thr phosphatase inhibitors, mainly type 1 protein phosphatase (PP1) and type 2A protein phosphatase (PP2A). Different effects have been reported at the cellular, molecular and genetic levels. In this study, changes in cell survival and cell mobility induced by OA, DTX-1 and DTX-2 were determined in epithelial cell lines of the colon and colon cancer. The cell viability results showed that tumoral cell lines were more resistant to toxins than the nontumoral cell line. The results of the functional assays for testing cell migration, evaluation of cell death and the expression of proteins associated with cell adhesion showed a dual effect of toxins since in the nontumoral cell line, a greater induction of cell death, presumably by anoikis, was detected. In the tumoral cell lines, there was an induction of a more aggressive phenotype characterized by increased resistance to toxins, increased migration and increased FAK activation. In tumoral cell lines of colon cancer, OA, DTX-1/DTX-2 induce a more aggressive phenotype.https://www.mdpi.com/2072-6651/12/3/179diarrhetic shellfish poisoning (dsp)okadaic acid (oa)dinophysistoxin-1 (dtx-1)dinophysistoxin-2 (dtx-2)colorectal cancer |
| spellingShingle | Danae Jiménez-Cárcamo Carlos García Héctor R. Contreras Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer Toxins diarrhetic shellfish poisoning (dsp) okadaic acid (oa) dinophysistoxin-1 (dtx-1) dinophysistoxin-2 (dtx-2) colorectal cancer |
| title | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
| title_full | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
| title_fullStr | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
| title_full_unstemmed | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
| title_short | Toxins of Okadaic Acid-Group Increase Malignant Properties in Cells of Colon Cancer |
| title_sort | toxins of okadaic acid group increase malignant properties in cells of colon cancer |
| topic | diarrhetic shellfish poisoning (dsp) okadaic acid (oa) dinophysistoxin-1 (dtx-1) dinophysistoxin-2 (dtx-2) colorectal cancer |
| url | https://www.mdpi.com/2072-6651/12/3/179 |
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