Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates.
The bone is a metabolically active organ which undergoes repeated remodeling cycles of bone resorption and formation. In this study, we revealed a robust and extremely long-lasting circadian rhythm in ex vivo culture maintained for over six months from the femoral bone of a PERIOD2(Luciferase) mouse...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3817244?pdf=render |
| _version_ | 1818921174096150528 |
|---|---|
| author | Naoki Okubo Yoichi Minami Hiroyoshi Fujiwara Yasuhiro Umemura Yoshiki Tsuchiya Toshiharu Shirai Ryo Oda Hitoshi Inokawa Toshikazu Kubo Kazuhiro Yagita |
| author_facet | Naoki Okubo Yoichi Minami Hiroyoshi Fujiwara Yasuhiro Umemura Yoshiki Tsuchiya Toshiharu Shirai Ryo Oda Hitoshi Inokawa Toshikazu Kubo Kazuhiro Yagita |
| author_sort | Naoki Okubo |
| collection | DOAJ |
| description | The bone is a metabolically active organ which undergoes repeated remodeling cycles of bone resorption and formation. In this study, we revealed a robust and extremely long-lasting circadian rhythm in ex vivo culture maintained for over six months from the femoral bone of a PERIOD2(Luciferase) mouse. Furthermore, we also identified robust circadian clocks in flat bones. High- or low-magnification real-time bioluminescence microscopic imaging revealed that the robust circadian rhythms emanated from the articular cartilage and the epiphyseal cartilage within the growth plate of juvenile animals. Stimulation by forskolin or dexamethasone treatment caused type 0 phase resetting, indicating canonical entraining properties of the bone clock. Together, our findings from long-term ex vivo culture revealed that "tissue-autonomous" circadian rhythm in the articular cartilage and the growth plate of femoral bone functions for several months even in an organ culture condition, and provided a useful in vitro assay system investigating the role of the biological clock in bone formation or development. |
| first_indexed | 2024-12-20T01:33:27Z |
| format | Article |
| id | doaj.art-f2ad8f2c58fb4e2ca64d78d530469f1a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:33:27Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f2ad8f2c58fb4e2ca64d78d530469f1a2022-12-21T19:58:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01811e7830610.1371/journal.pone.0078306Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates.Naoki OkuboYoichi MinamiHiroyoshi FujiwaraYasuhiro UmemuraYoshiki TsuchiyaToshiharu ShiraiRyo OdaHitoshi InokawaToshikazu KuboKazuhiro YagitaThe bone is a metabolically active organ which undergoes repeated remodeling cycles of bone resorption and formation. In this study, we revealed a robust and extremely long-lasting circadian rhythm in ex vivo culture maintained for over six months from the femoral bone of a PERIOD2(Luciferase) mouse. Furthermore, we also identified robust circadian clocks in flat bones. High- or low-magnification real-time bioluminescence microscopic imaging revealed that the robust circadian rhythms emanated from the articular cartilage and the epiphyseal cartilage within the growth plate of juvenile animals. Stimulation by forskolin or dexamethasone treatment caused type 0 phase resetting, indicating canonical entraining properties of the bone clock. Together, our findings from long-term ex vivo culture revealed that "tissue-autonomous" circadian rhythm in the articular cartilage and the growth plate of femoral bone functions for several months even in an organ culture condition, and provided a useful in vitro assay system investigating the role of the biological clock in bone formation or development.http://europepmc.org/articles/PMC3817244?pdf=render |
| spellingShingle | Naoki Okubo Yoichi Minami Hiroyoshi Fujiwara Yasuhiro Umemura Yoshiki Tsuchiya Toshiharu Shirai Ryo Oda Hitoshi Inokawa Toshikazu Kubo Kazuhiro Yagita Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. PLoS ONE |
| title | Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. |
| title_full | Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. |
| title_fullStr | Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. |
| title_full_unstemmed | Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. |
| title_short | Prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates. |
| title_sort | prolonged bioluminescence monitoring in mouse ex vivo bone culture revealed persistent circadian rhythms in articular cartilages and growth plates |
| url | http://europepmc.org/articles/PMC3817244?pdf=render |
| work_keys_str_mv | AT naokiokubo prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT yoichiminami prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT hiroyoshifujiwara prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT yasuhiroumemura prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT yoshikitsuchiya prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT toshiharushirai prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT ryooda prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT hitoshiinokawa prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT toshikazukubo prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates AT kazuhiroyagita prolongedbioluminescencemonitoringinmouseexvivoboneculturerevealedpersistentcircadianrhythmsinarticularcartilagesandgrowthplates |