Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic <i>Pseudomonas</i> sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/13/7069 |
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| author | Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas |
| author_facet | Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas |
| author_sort | Erika Alina Ordóñez-León |
| collection | DOAJ |
| description | This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic <i>Pseudomonas</i> sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (<i>p</i> ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (<i>p</i> ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of <i>BCL2</i>, <i>AQP3</i>, <i>CX43,</i> and <i>SOD1</i> genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher <i>BAX</i> gene expression, whereas treatment with 100 µg/mL EPS ID1 returned <i>BAX</i> levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. |
| first_indexed | 2024-03-09T21:51:24Z |
| format | Article |
| id | doaj.art-f2af604f4f4043e0ba5100a1883fa18d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:51:24Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f2af604f4f4043e0ba5100a1883fa18d2023-11-23T20:07:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313706910.3390/ijms23137069Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine EmbryosErika Alina Ordóñez-León0Iris Martínez-Rodero1Tania García-Martínez2Manel López-Béjar3Marc Yeste4Elena Mercade5Teresa Mogas6Department of Animal Medicine and Surgery, Autonomous University of Barcelona, Cerdanyola del Vallès, ES-08193 Barcelona, SpainDepartment of Animal Medicine and Surgery, Autonomous University of Barcelona, Cerdanyola del Vallès, ES-08193 Barcelona, SpainDepartment of Animal Medicine and Surgery, Autonomous University of Barcelona, Cerdanyola del Vallès, ES-08193 Barcelona, SpainDepartment of Animal Health and Anatomy, Autonomous University of Barcelona, Cerdanyola del Vallès, ES-08193 Barcelona, SpainDepartment of Biology, Institute of Food and Agricultural Technology, University of Girona, ES-17004 Girona, SpainDepartment of Biology, Health and Environment, University of Barcelona, ES-08007 Barcelona, SpainDepartment of Animal Medicine and Surgery, Autonomous University of Barcelona, Cerdanyola del Vallès, ES-08193 Barcelona, SpainThis study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic <i>Pseudomonas</i> sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (<i>p</i> ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (<i>p</i> ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of <i>BCL2</i>, <i>AQP3</i>, <i>CX43,</i> and <i>SOD1</i> genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher <i>BAX</i> gene expression, whereas treatment with 100 µg/mL EPS ID1 returned <i>BAX</i> levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis.https://www.mdpi.com/1422-0067/23/13/7069cryopreservationblastocysttotal cell numberinner cell massTUNELembryo development |
| spellingShingle | Erika Alina Ordóñez-León Iris Martínez-Rodero Tania García-Martínez Manel López-Béjar Marc Yeste Elena Mercade Teresa Mogas Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos International Journal of Molecular Sciences cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development |
| title | Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
| title_full | Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
| title_fullStr | Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
| title_full_unstemmed | Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
| title_short | Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos |
| title_sort | exopolysaccharide id1 improves post warming outcomes after vitrification of in vitro produced bovine embryos |
| topic | cryopreservation blastocyst total cell number inner cell mass TUNEL embryo development |
| url | https://www.mdpi.com/1422-0067/23/13/7069 |
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