Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam.
Context: Caesalpinia bahamensis Lam. is a medicinal plant used by the Cuban population to treat renal and hepatic diseases. However, this species lacks scientific studies that support its biological applications. Aims: To evaluate the chemical composition and the antioxidant capacity of fractions...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2019-01-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol7/jppres18.421_7.1.12.pdf |
| _version_ | 1811331681768964096 |
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| author | Alejandro Felipe González Ivones Hernández Balmaseda Yamilet I. Gutiérrez Gaitén Ramón Scull Lizama Laura M. Carmenate García Luc Pieters Idania Rodeiro Guerra René Delgado Hernández |
| author_facet | Alejandro Felipe González Ivones Hernández Balmaseda Yamilet I. Gutiérrez Gaitén Ramón Scull Lizama Laura M. Carmenate García Luc Pieters Idania Rodeiro Guerra René Delgado Hernández |
| author_sort | Alejandro Felipe González |
| collection | DOAJ |
| description | Context: Caesalpinia bahamensis Lam. is a medicinal plant used by the Cuban population to treat renal and hepatic diseases. However, this species lacks scientific studies that support its biological applications.
Aims: To evaluate the chemical composition and the antioxidant capacity of fractions obtained from the stem of Caesalpinia bahamensis Lam.
Methods: A continuous extraction of the stem was made by maceration using a battery of solvents of increasing polarity: chloroform, ethyl acetate and methanol. All fractions were analyzed by TLC and phytochemical screening. The compounds of the chloroform fraction were identified by GC/MS, while the ethyl acetate and methanol fractions were characterized by UV-Vis spectroscopy. The antioxidant capacity was evaluated by the DPPH and FRAP assays.
Results: Ten compounds were identified by GC/MS of the chloroform fraction, associated with fatty acids, terpenoids and phytosterols. The major compounds of this fraction were octacosanol, monopalmitin and palmitic acid. The presence of flavonoids in the ethyl acetate and methanol fractions was demonstrated by phytochemical screening, TLC and UV spectroscopy. The three fractions showed antioxidant capacity in the DPPH assay, with the methanol fraction (IC50=11.1 µg/mL) being the most active. The ethyl acetate fraction (equivalent to 100.7 µmol ascorbic acid) and the methanol fraction (equivalent to 37.3 µmol ascorbic acid) showed antioxidant capacity in the FRAP assay at concentrations of 125 µg/mL and 1000 µg/mL, respectively.
Conclusions: The fractions evaluated showed antioxidant capacity in the DPPH and FRAP assays, possibly associated with the presence of phenols and flavonoids. |
| first_indexed | 2024-04-13T16:24:19Z |
| format | Article |
| id | doaj.art-f2afa84b914a415f919272c165417cfb |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-13T16:24:19Z |
| publishDate | 2019-01-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-f2afa84b914a415f919272c165417cfb2022-12-22T02:39:47ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502019-01-01711220Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam.Alejandro Felipe González0Ivones Hernández Balmaseda1Yamilet I. Gutiérrez Gaitén2Ramón Scull Lizama3Laura M. Carmenate García4Luc Pieters5Idania Rodeiro Guerra6René Delgado Hernández7Departamento de Farmacia. Instituto de Farmacia y Alimentos. Universidad de La Habana, Calle 222 No. 2317 entre 23 y 31. La Lisa, La Habana, Cuba.Departamento de Farmacología. Instituto de Ciencias del Mar, La Habana, Cuba.Departamento de Farmacia. Instituto de Farmacia y Alimentos. Universidad de La Habana, Calle 222 No. 2317 entre 23 y 31. La Lisa, La Habana, Cuba.Departamento de Farmacia. Instituto de Farmacia y Alimentos. Universidad de La Habana, Calle 222 No. 2317 entre 23 y 31. La Lisa, La Habana, Cuba.Empresa Importadora, Exportadora, Distribuidora y Comercializadora de Productos Farmacéuticos, La Habana, Cuba.Natural Products & Food Research and Analysis (NatuRA). Department of Pharmaceutical Sciences. University of Antwerp, Belgium.Departamento de Farmacología. Instituto de Ciencias del Mar, La Habana, Cuba.Centro de Estudios para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos. Universidad de La Habana. La Habana, Cuba.Context: Caesalpinia bahamensis Lam. is a medicinal plant used by the Cuban population to treat renal and hepatic diseases. However, this species lacks scientific studies that support its biological applications. Aims: To evaluate the chemical composition and the antioxidant capacity of fractions obtained from the stem of Caesalpinia bahamensis Lam. Methods: A continuous extraction of the stem was made by maceration using a battery of solvents of increasing polarity: chloroform, ethyl acetate and methanol. All fractions were analyzed by TLC and phytochemical screening. The compounds of the chloroform fraction were identified by GC/MS, while the ethyl acetate and methanol fractions were characterized by UV-Vis spectroscopy. The antioxidant capacity was evaluated by the DPPH and FRAP assays. Results: Ten compounds were identified by GC/MS of the chloroform fraction, associated with fatty acids, terpenoids and phytosterols. The major compounds of this fraction were octacosanol, monopalmitin and palmitic acid. The presence of flavonoids in the ethyl acetate and methanol fractions was demonstrated by phytochemical screening, TLC and UV spectroscopy. The three fractions showed antioxidant capacity in the DPPH assay, with the methanol fraction (IC50=11.1 µg/mL) being the most active. The ethyl acetate fraction (equivalent to 100.7 µmol ascorbic acid) and the methanol fraction (equivalent to 37.3 µmol ascorbic acid) showed antioxidant capacity in the FRAP assay at concentrations of 125 µg/mL and 1000 µg/mL, respectively. Conclusions: The fractions evaluated showed antioxidant capacity in the DPPH and FRAP assays, possibly associated with the presence of phenols and flavonoids.http://jppres.com/jppres/pdf/vol7/jppres18.421_7.1.12.pdfantioxidant activityCaesalpinia bahamensisfatty acidsflavonoidsGC/MS |
| spellingShingle | Alejandro Felipe González Ivones Hernández Balmaseda Yamilet I. Gutiérrez Gaitén Ramón Scull Lizama Laura M. Carmenate García Luc Pieters Idania Rodeiro Guerra René Delgado Hernández Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. Journal of Pharmacy & Pharmacognosy Research antioxidant activity Caesalpinia bahamensis fatty acids flavonoids GC/MS |
| title | Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. |
| title_full | Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. |
| title_fullStr | Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. |
| title_full_unstemmed | Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. |
| title_short | Phytochemical study and antioxidant capacity of three fractions from the stem of Caesalpinia bahamensis Lam. |
| title_sort | phytochemical study and antioxidant capacity of three fractions from the stem of caesalpinia bahamensis lam |
| topic | antioxidant activity Caesalpinia bahamensis fatty acids flavonoids GC/MS |
| url | http://jppres.com/jppres/pdf/vol7/jppres18.421_7.1.12.pdf |
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