Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography
Abstract Background The measurement of liver stiffness (LS) and spleen stiffness (SS) based on ultrasound elastography can be used for non-invasive assessment of portal hypertension (PH). However, there are few studies on the corresponding mechanism of increased spleen stiffness. Our aim was to use...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Medical Imaging |
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| Online Access: | https://doi.org/10.1186/s12880-022-00786-6 |
| _version_ | 1818273449938780160 |
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| author | YongJian Chen JingYun Li Qin Zhou GuoRong Lyu ShiLin Li |
| author_facet | YongJian Chen JingYun Li Qin Zhou GuoRong Lyu ShiLin Li |
| author_sort | YongJian Chen |
| collection | DOAJ |
| description | Abstract Background The measurement of liver stiffness (LS) and spleen stiffness (SS) based on ultrasound elastography can be used for non-invasive assessment of portal hypertension (PH). However, there are few studies on the corresponding mechanism of increased spleen stiffness. Our aim was to use two-dimensional shear wave elastrography (2D-SWE) to evaluate the relationship between LS and SS and the severity of PH in rats. And explore the mechanism of the increase of LS and SS in PH. Methods Sixty male Sprague–Dawley rats were randomly divided into portal hypertension (PH group, n = 45) and normal control (NC group, n = 15). At 12 weeks, LS and SS was detected by 2D-SWE in vivo. Related hemodynamic parameters and portal vein pressure (PVP) was measured. Spleen and liver 2D-SWE detection was performed again after sacrifice. Pathological changes were observed. Results The SS and LS were increased in PH group (P < 0.05). The SS decreased after sacrifice, and what's more the magnitude of SS decline significantly higher in PH group than in NC group (P < 0.05). The correlation between SS and PVP is stronger than LS (r = 0.624, P < 0.001). SS has positive correlation with indexes of hyperdynamic circulation, but LS was weakly. The correlation between SS and the pathological grade (r = 0.633, P < 0.001) was lower than that in LS (r = 0.905, P < 0.001). Multiple linear regression analysis revealed that SS, portal vein inner diameter (PVD) and splenic vein blood flow velocity (SVV) were significantly associated with PH. Conclusions Spleen and liver measurement by 2D-SWE may be helpful in evaluating PVP. The correlation between SS and PVP is stronger than LS in rats measured by 2D-SWE. Hemodynamic circulation are important in the elevation of SS with portal hypertension. Pathological changes also have a degree of influence, but have more significance for the elevation of LS. SS may be a more effective noninvasive predictor of PH than LS. |
| first_indexed | 2024-12-12T21:58:09Z |
| format | Article |
| id | doaj.art-f2b02db28be740ba9f51f54031c92481 |
| institution | Directory Open Access Journal |
| issn | 1471-2342 |
| language | English |
| last_indexed | 2024-12-12T21:58:09Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Imaging |
| spelling | doaj.art-f2b02db28be740ba9f51f54031c924812022-12-22T00:10:35ZengBMCBMC Medical Imaging1471-23422022-04-012211710.1186/s12880-022-00786-6Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastographyYongJian Chen0JingYun Li1Qin Zhou2GuoRong Lyu3ShiLin Li4Department of Ultrasound, The Second Affiliated Hospital of Fujian Medical UniversityMaternal and Child Health Service Application Technology Collaborative Innovation Center, Quanzhou Medical CollegeDepartment of Ultrasound, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Fujian Medical UniversityDepartment of Ultrasound, The Second Affiliated Hospital of Fujian Medical UniversityAbstract Background The measurement of liver stiffness (LS) and spleen stiffness (SS) based on ultrasound elastography can be used for non-invasive assessment of portal hypertension (PH). However, there are few studies on the corresponding mechanism of increased spleen stiffness. Our aim was to use two-dimensional shear wave elastrography (2D-SWE) to evaluate the relationship between LS and SS and the severity of PH in rats. And explore the mechanism of the increase of LS and SS in PH. Methods Sixty male Sprague–Dawley rats were randomly divided into portal hypertension (PH group, n = 45) and normal control (NC group, n = 15). At 12 weeks, LS and SS was detected by 2D-SWE in vivo. Related hemodynamic parameters and portal vein pressure (PVP) was measured. Spleen and liver 2D-SWE detection was performed again after sacrifice. Pathological changes were observed. Results The SS and LS were increased in PH group (P < 0.05). The SS decreased after sacrifice, and what's more the magnitude of SS decline significantly higher in PH group than in NC group (P < 0.05). The correlation between SS and PVP is stronger than LS (r = 0.624, P < 0.001). SS has positive correlation with indexes of hyperdynamic circulation, but LS was weakly. The correlation between SS and the pathological grade (r = 0.633, P < 0.001) was lower than that in LS (r = 0.905, P < 0.001). Multiple linear regression analysis revealed that SS, portal vein inner diameter (PVD) and splenic vein blood flow velocity (SVV) were significantly associated with PH. Conclusions Spleen and liver measurement by 2D-SWE may be helpful in evaluating PVP. The correlation between SS and PVP is stronger than LS in rats measured by 2D-SWE. Hemodynamic circulation are important in the elevation of SS with portal hypertension. Pathological changes also have a degree of influence, but have more significance for the elevation of LS. SS may be a more effective noninvasive predictor of PH than LS.https://doi.org/10.1186/s12880-022-00786-6Portal hypertensionTwo-dimensional shear wave elastographySpleen stiffnessLiver stiffnessHemodynamics |
| spellingShingle | YongJian Chen JingYun Li Qin Zhou GuoRong Lyu ShiLin Li Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography BMC Medical Imaging Portal hypertension Two-dimensional shear wave elastography Spleen stiffness Liver stiffness Hemodynamics |
| title | Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography |
| title_full | Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography |
| title_fullStr | Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography |
| title_full_unstemmed | Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography |
| title_short | Detection of liver and spleen stiffness in rats with portal hypertension by two-dimensional shear wave elastography |
| title_sort | detection of liver and spleen stiffness in rats with portal hypertension by two dimensional shear wave elastography |
| topic | Portal hypertension Two-dimensional shear wave elastography Spleen stiffness Liver stiffness Hemodynamics |
| url | https://doi.org/10.1186/s12880-022-00786-6 |
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