Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch).
Buprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus) as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0....
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3789747?pdf=render |
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| author | Kasi Marimuthu Narmataa Muthu Rathinam Xavier Jesu Arockiaraj M Aminur Rahman Sreeramanan Subramaniam |
| author_facet | Kasi Marimuthu Narmataa Muthu Rathinam Xavier Jesu Arockiaraj M Aminur Rahman Sreeramanan Subramaniam |
| author_sort | Kasi Marimuthu |
| collection | DOAJ |
| description | Buprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus) as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0.05, 0.5, 5, 25, 50 and 100 mg/L) of buprofezin. Each concentration was assessed in five replicates. Eggs were artificially fertilized and 200 eggs and larvae were subjected to a static bath treatment for all the concentrations. The mortality of embryos was significantly increased with increasing buprofezin concentrations from 5 to 100 mg/L (p< 0.05). However, the mortality was not significantly different (p<0.05) among the following concentrations: 0 (control), 0.05, 0.5 and 5 mg/L. Data obtained from the buprofezin acute toxicity tests were evaluated using probit analysis. The 24 h LC50 value (with 95% confidence limits) of buprofezin for embryos was estimated to be 6.725 (3.167-15.017) mg/L. The hatching of fish embryos was recorded as 68.8, 68.9, 66.9, 66.4, 26.9, 25.1 and 0.12% in response to 7 different concentrations of buprofezin, respectively. The mortality rate of larvae significantly (p<0.05) increased with increasing buprofezin concentrations exposed to 24-48 h. The 24 and 48 h LC50 values (with 95% confidence limits) of buprofezin for the larvae was estimated to be 5.702 (3.198-8.898) and 4.642 (3.264-6.287) mg/L respectively. There were no significant differences (p>0.05) in the LC50 values obtained at 24 and 48 h exposure times. Malformations were observed when the embryos and larvae exposed to more than 5 mg/L. The results emerged from the study suggest that even the low concentration (5 mg/L) of buprofezin in the aquatic environment may have adverse effect on the early embryonic and larval development of African catfish. |
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| language | English |
| last_indexed | 2024-12-11T21:03:45Z |
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| spelling | doaj.art-f2b09f477e61458587128085c2b3d43f2022-12-22T00:50:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01810e7554510.1371/journal.pone.0075545Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch).Kasi MarimuthuNarmataa MuthuRathinam XavierJesu ArockiarajM Aminur RahmanSreeramanan SubramaniamBuprofezin is an insect growth regulator and widely used insecticide in Malaysia. The present study evaluated the toxic effects of buprofezin on the embryo and larvae of African catfish (Clarias gariepinus) as a model organism. The embryos and larvae were exposed to 7 different concentrations (0, 0.05, 0.5, 5, 25, 50 and 100 mg/L) of buprofezin. Each concentration was assessed in five replicates. Eggs were artificially fertilized and 200 eggs and larvae were subjected to a static bath treatment for all the concentrations. The mortality of embryos was significantly increased with increasing buprofezin concentrations from 5 to 100 mg/L (p< 0.05). However, the mortality was not significantly different (p<0.05) among the following concentrations: 0 (control), 0.05, 0.5 and 5 mg/L. Data obtained from the buprofezin acute toxicity tests were evaluated using probit analysis. The 24 h LC50 value (with 95% confidence limits) of buprofezin for embryos was estimated to be 6.725 (3.167-15.017) mg/L. The hatching of fish embryos was recorded as 68.8, 68.9, 66.9, 66.4, 26.9, 25.1 and 0.12% in response to 7 different concentrations of buprofezin, respectively. The mortality rate of larvae significantly (p<0.05) increased with increasing buprofezin concentrations exposed to 24-48 h. The 24 and 48 h LC50 values (with 95% confidence limits) of buprofezin for the larvae was estimated to be 5.702 (3.198-8.898) and 4.642 (3.264-6.287) mg/L respectively. There were no significant differences (p>0.05) in the LC50 values obtained at 24 and 48 h exposure times. Malformations were observed when the embryos and larvae exposed to more than 5 mg/L. The results emerged from the study suggest that even the low concentration (5 mg/L) of buprofezin in the aquatic environment may have adverse effect on the early embryonic and larval development of African catfish.http://europepmc.org/articles/PMC3789747?pdf=render |
| spellingShingle | Kasi Marimuthu Narmataa Muthu Rathinam Xavier Jesu Arockiaraj M Aminur Rahman Sreeramanan Subramaniam Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). PLoS ONE |
| title | Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). |
| title_full | Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). |
| title_fullStr | Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). |
| title_full_unstemmed | Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). |
| title_short | Toxicity of buprofezin on the survival of embryo and larvae of African catfish, Clarias gariepinus (Bloch). |
| title_sort | toxicity of buprofezin on the survival of embryo and larvae of african catfish clarias gariepinus bloch |
| url | http://europepmc.org/articles/PMC3789747?pdf=render |
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