Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts
Non-invasive physical plasma (NIPP), an electrically conductive gas, is playing an increasingly important role in medicine due to its antimicrobial and regenerative properties. However, NIPP is not yet well established in dentistry, although it has promising potential, especially for periodontologic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16156 |
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| author | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Alexander Mustea Sigrun Eick James Deschner Marjan Nokhbehsaim Franz-Josef Kramer |
| author_facet | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Alexander Mustea Sigrun Eick James Deschner Marjan Nokhbehsaim Franz-Josef Kramer |
| author_sort | Benedikt Eggers |
| collection | DOAJ |
| description | Non-invasive physical plasma (NIPP), an electrically conductive gas, is playing an increasingly important role in medicine due to its antimicrobial and regenerative properties. However, NIPP is not yet well established in dentistry, although it has promising potential, especially for periodontological applications. The aim of the present study was to investigate the effect of NIPP on a commercially available human gingival fibroblast (HGF) cell line and primary HGFs in the presence of periodontitis-associated bacteria. First, primary HGFs from eight patients were characterised by immunofluorescence, and cell numbers were examined by an automatic cell counter over 5 days. Then, HGFs that were preincubated with <i>Fusobacterium nucleatum</i> (<i>F.n.</i>) were treated with NIPP. Afterwards, the IL-6 and IL-8 levels in the cell supernatants were determined by ELISA. In HGFs, <i>F.n.</i> caused a significant increase in IL-6 and IL-8, and this <i>F.n.</i>-induced upregulation of both cytokines was counteracted by NIPP, suggesting a beneficial effect of physical plasma on periodontal cells in a microbial environment. The application of NIPP in periodontal therapy could therefore represent a novel and promising strategy and deserves further investigation. |
| first_indexed | 2024-03-09T16:46:29Z |
| format | Article |
| id | doaj.art-f2bd8141961343a88c07a2447a2e7b98 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:46:29Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f2bd8141961343a88c07a2447a2e7b982023-11-24T14:46:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221615610.3390/ijms242216156Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival FibroblastsBenedikt Eggers0Matthias Bernhard Stope1Jana Marciniak2Alexander Mustea3Sigrun Eick4James Deschner5Marjan Nokhbehsaim6Franz-Josef Kramer7Department of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Orthodontics, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53127 Bonn, GermanyDepartment of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, SwitzerlandDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, GermanySection of Experimental Dento-Maxillo-Facial Medicine, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanyNon-invasive physical plasma (NIPP), an electrically conductive gas, is playing an increasingly important role in medicine due to its antimicrobial and regenerative properties. However, NIPP is not yet well established in dentistry, although it has promising potential, especially for periodontological applications. The aim of the present study was to investigate the effect of NIPP on a commercially available human gingival fibroblast (HGF) cell line and primary HGFs in the presence of periodontitis-associated bacteria. First, primary HGFs from eight patients were characterised by immunofluorescence, and cell numbers were examined by an automatic cell counter over 5 days. Then, HGFs that were preincubated with <i>Fusobacterium nucleatum</i> (<i>F.n.</i>) were treated with NIPP. Afterwards, the IL-6 and IL-8 levels in the cell supernatants were determined by ELISA. In HGFs, <i>F.n.</i> caused a significant increase in IL-6 and IL-8, and this <i>F.n.</i>-induced upregulation of both cytokines was counteracted by NIPP, suggesting a beneficial effect of physical plasma on periodontal cells in a microbial environment. The application of NIPP in periodontal therapy could therefore represent a novel and promising strategy and deserves further investigation.https://www.mdpi.com/1422-0067/24/22/16156non-invasive physical plasmacold plasmanon-thermal plasmaantimicrobial effectinflammationgingival fibroblasts |
| spellingShingle | Benedikt Eggers Matthias Bernhard Stope Jana Marciniak Alexander Mustea Sigrun Eick James Deschner Marjan Nokhbehsaim Franz-Josef Kramer Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts International Journal of Molecular Sciences non-invasive physical plasma cold plasma non-thermal plasma antimicrobial effect inflammation gingival fibroblasts |
| title | Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts |
| title_full | Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts |
| title_fullStr | Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts |
| title_full_unstemmed | Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts |
| title_short | Non-Invasive Physical Plasma Reduces the Inflammatory Response in Microbially Prestimulated Human Gingival Fibroblasts |
| title_sort | non invasive physical plasma reduces the inflammatory response in microbially prestimulated human gingival fibroblasts |
| topic | non-invasive physical plasma cold plasma non-thermal plasma antimicrobial effect inflammation gingival fibroblasts |
| url | https://www.mdpi.com/1422-0067/24/22/16156 |
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