Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode
In the mitochondrial intermembrane space, the redox protein heme-Cytochrome c (Cyt c) acts as an electron carrier. The translocation of Cyt c out of mitochondria triggers programmed cell death. In this study, direct electrochemistry of Cyt c adsorbed onto the surface of a graphene-modified electrode...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Iranian Chemical Society
2022-11-01
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| Series: | Nanochemistry Research |
| Subjects: | |
| Online Access: | http://www.nanochemres.org/article_159181_936e92378db2ef2b8334d156050c2436.pdf |
| Summary: | In the mitochondrial intermembrane space, the redox protein heme-Cytochrome c (Cyt c) acts as an electron carrier. The translocation of Cyt c out of mitochondria triggers programmed cell death. In this study, direct electrochemistry of Cyt c adsorbed onto the surface of a graphene-modified electrode was investigated. Owing to the high electron mobility of one-atom thick graphene, it serves as a unique platform for facilitating direct electron transfer of proteins. The redox peak currents of the Cyt c-immobilized graphene increased linearly with increasing the scan rate, revealing a surface-controlled electrochemical process. The enzyme-mimetic activity of the Cyt c-immobilized graphene in the electroreduction of H2O2, from 2.0 µM to 4.0 mM with a detection limit of 0.4 µM, demonstrated that the graphene maintained the bioactivity of Cyt c. This intriguing enzyme-liked catalytic activity makes the Cyt c-modified graphene electrode a suitable candidate for fabricating H2O2 sensors. This direct electron-based electroreduction opens a new horizon for highly sensitive targeted bioanalysis with a functional nanomaterial design. |
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| ISSN: | 2538-4279 2423-818X |