Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode
In the mitochondrial intermembrane space, the redox protein heme-Cytochrome c (Cyt c) acts as an electron carrier. The translocation of Cyt c out of mitochondria triggers programmed cell death. In this study, direct electrochemistry of Cyt c adsorbed onto the surface of a graphene-modified electrode...
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| Format: | Article |
| Language: | English |
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Iranian Chemical Society
2022-11-01
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| Series: | Nanochemistry Research |
| Subjects: | |
| Online Access: | http://www.nanochemres.org/article_159181_936e92378db2ef2b8334d156050c2436.pdf |
| _version_ | 1811171981940228096 |
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| author | Fatemeh Norouz-Sarvestani Seyyed Mehdi Khoshfetrat Abdolkarim Abbaspour |
| author_facet | Fatemeh Norouz-Sarvestani Seyyed Mehdi Khoshfetrat Abdolkarim Abbaspour |
| author_sort | Fatemeh Norouz-Sarvestani |
| collection | DOAJ |
| description | In the mitochondrial intermembrane space, the redox protein heme-Cytochrome c (Cyt c) acts as an electron carrier. The translocation of Cyt c out of mitochondria triggers programmed cell death. In this study, direct electrochemistry of Cyt c adsorbed onto the surface of a graphene-modified electrode was investigated. Owing to the high electron mobility of one-atom thick graphene, it serves as a unique platform for facilitating direct electron transfer of proteins. The redox peak currents of the Cyt c-immobilized graphene increased linearly with increasing the scan rate, revealing a surface-controlled electrochemical process. The enzyme-mimetic activity of the Cyt c-immobilized graphene in the electroreduction of H2O2, from 2.0 µM to 4.0 mM with a detection limit of 0.4 µM, demonstrated that the graphene maintained the bioactivity of Cyt c. This intriguing enzyme-liked catalytic activity makes the Cyt c-modified graphene electrode a suitable candidate for fabricating H2O2 sensors. This direct electron-based electroreduction opens a new horizon for highly sensitive targeted bioanalysis with a functional nanomaterial design. |
| first_indexed | 2024-04-10T17:24:21Z |
| format | Article |
| id | doaj.art-f2bfda0c9c314cd39df27c1cef58e6ac |
| institution | Directory Open Access Journal |
| issn | 2538-4279 2423-818X |
| language | English |
| last_indexed | 2024-04-10T17:24:21Z |
| publishDate | 2022-11-01 |
| publisher | Iranian Chemical Society |
| record_format | Article |
| series | Nanochemistry Research |
| spelling | doaj.art-f2bfda0c9c314cd39df27c1cef58e6ac2023-02-05T07:24:02ZengIranian Chemical SocietyNanochemistry Research2538-42792423-818X2022-11-0172798410.22036/ncr.2022.02.003159181Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrodeFatemeh Norouz-Sarvestani0Seyyed Mehdi Khoshfetrat1Abdolkarim Abbaspour2Department of Chemistry, College of Sciences, Shiraz University, Shiraz, IranDepartment of Chemistry, Faculty of Basic Science, Ayatollah Boroujerdi University, Boroujerd, IranDepartment of Chemistry, College of Sciences, Shiraz University, Shiraz, IranIn the mitochondrial intermembrane space, the redox protein heme-Cytochrome c (Cyt c) acts as an electron carrier. The translocation of Cyt c out of mitochondria triggers programmed cell death. In this study, direct electrochemistry of Cyt c adsorbed onto the surface of a graphene-modified electrode was investigated. Owing to the high electron mobility of one-atom thick graphene, it serves as a unique platform for facilitating direct electron transfer of proteins. The redox peak currents of the Cyt c-immobilized graphene increased linearly with increasing the scan rate, revealing a surface-controlled electrochemical process. The enzyme-mimetic activity of the Cyt c-immobilized graphene in the electroreduction of H2O2, from 2.0 µM to 4.0 mM with a detection limit of 0.4 µM, demonstrated that the graphene maintained the bioactivity of Cyt c. This intriguing enzyme-liked catalytic activity makes the Cyt c-modified graphene electrode a suitable candidate for fabricating H2O2 sensors. This direct electron-based electroreduction opens a new horizon for highly sensitive targeted bioanalysis with a functional nanomaterial design.http://www.nanochemres.org/article_159181_936e92378db2ef2b8334d156050c2436.pdfgraphenecytochrome chydrogen peroxideelectrochemical biosensor |
| spellingShingle | Fatemeh Norouz-Sarvestani Seyyed Mehdi Khoshfetrat Abdolkarim Abbaspour Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode Nanochemistry Research graphene cytochrome c hydrogen peroxide electrochemical biosensor |
| title | Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode |
| title_full | Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode |
| title_fullStr | Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode |
| title_full_unstemmed | Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode |
| title_short | Electroreduction of Hydrogen peroxide using Direct electrocatalysis of Cytochrome c on the a graphene-modified electrode |
| title_sort | electroreduction of hydrogen peroxide using direct electrocatalysis of cytochrome c on the a graphene modified electrode |
| topic | graphene cytochrome c hydrogen peroxide electrochemical biosensor |
| url | http://www.nanochemres.org/article_159181_936e92378db2ef2b8334d156050c2436.pdf |
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