Mitogenomic Characterization and Phylogenetic Placement of African Hind, <i>Cephalopholis taeniops</i>: Shedding Light on the Evolution of Groupers (Serranidae: Epinephelinae)
The global exploration of evolutionary trends in groupers, based on mitogenomes, is currently underway. This research extensively investigates the structure of and variations in <i>Cephalopholis</i> species mitogenomes, along with their phylogenetic relationships, focusing specifically o...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/3/1822 |
| Summary: | The global exploration of evolutionary trends in groupers, based on mitogenomes, is currently underway. This research extensively investigates the structure of and variations in <i>Cephalopholis</i> species mitogenomes, along with their phylogenetic relationships, focusing specifically on <i>Cephalopholis taeniops</i> from the Eastern Atlantic Ocean. The generated mitogenome spans 16,572 base pairs and exhibits a gene order analogous to that of the ancestral teleost’s, featuring 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich control region. The mitogenome of <i>C. taeniops</i> displays an AT bias (54.99%), aligning with related species. The majority of PCGs in the mitogenome initiate with the start codon ATG, with the exceptions being <i>COI</i> (GTG) and <i>atp6</i> (TTG). The relative synonymous codon usage analysis revealed the maximum abundance of leucine, proline, serine, and threonine. The nonsynonymous/synonymous ratios were <1, which indicates a strong negative selection among all PCGs of the <i>Cephalopholis</i> species. In <i>C. taeniops</i>, the prevalent transfer RNAs display conventional cloverleaf secondary structures, except for tRNA-serine (GCT), which lacks a dihydrouracil (DHU) stem. A comparative examination of conserved domains and sequence blocks across various <i>Cephalopholis</i> species indicates noteworthy variations in length and nucleotide diversity. Maximum likelihood, neighbor-joining, and Bayesian phylogenetic analyses, employing the concatenated PCGs and a combination of PCGs + rRNAs, distinctly separate all <i>Cephalopholis</i> species, including <i>C. taeniops</i>. Overall, these findings deepen our understanding of evolutionary relationships among serranid groupers, emphasizing the significance of structural considerations in mitogenomic analyses. |
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| ISSN: | 1661-6596 1422-0067 |