bFGF-incorporated composite biomaterial for bone regeneration
Injectable biomaterials obtain increasing attention for the repair of bone defect due to the advantage of defect margin adaptability and easy handling. Our previous studies found that RADA16 peptide hydrogel could significantly promote bone formation. In order to improve the mechanical force, RADA16...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522000909 |
| _version_ | 1818759764464631808 |
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| author | Bin He Muzi Zhang Lifeng Yin Zhengxue Quan Yunsheng Ou Wei Huang |
| author_facet | Bin He Muzi Zhang Lifeng Yin Zhengxue Quan Yunsheng Ou Wei Huang |
| author_sort | Bin He |
| collection | DOAJ |
| description | Injectable biomaterials obtain increasing attention for the repair of bone defect due to the advantage of defect margin adaptability and easy handling. Our previous studies found that RADA16 peptide hydrogel could significantly promote bone formation. In order to improve the mechanical force, RADA16 peptide hydrogel was used to fill the porous calcium sulfate/nano-hydroxyapatite (CaSO4/HA) cement biomaterial for the fabrication of RADA16/CaSO4/HA composite biomaterial. Especially, RADA16 peptide hydrogel had the ability to slowly release growth factors for bone regeneration. Here, RADA16/CaSO4/HA composite biomaterials afforded the controlled and sustainable release of basic fibroblast growth factor (bFGF) for more than 32 days. Compared to RADA16/CaSO4/HA biomaterial, RADA16 + bFGF/CaSO4/HA composite biomaterial could further improve in vitro osteogenic differentiation of MC3T3 cells as evidenced by the increased expression of osteogenic markers (i.e. runt-related transcription factor 2 [Runx2], osteocalcin [OCN] and alkaline phosphatase [ALP]) and in vivo bone formation of femoral condyle defects which were confirmed by histological staining and micro-CT analyses. These indicate that bFGF-incorporated RADA16/CaSO4/HA can further improve bone formation through the controlled release of bFGF, which provides a novel injectable biomaterial design to specifically release growth factors for bone defects. |
| first_indexed | 2024-12-18T06:47:55Z |
| format | Article |
| id | doaj.art-f2cb7a0ebcc54ae0a28dc3e05d360423 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-18T06:47:55Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-f2cb7a0ebcc54ae0a28dc3e05d3604232022-12-21T21:17:26ZengElsevierMaterials & Design0264-12752022-03-01215110469bFGF-incorporated composite biomaterial for bone regenerationBin He0Muzi Zhang1Lifeng Yin2Zhengxue Quan3Yunsheng Ou4Wei Huang5Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Orthopedic Laboratory of Chongqing Medical University, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Orthopedic Laboratory of Chongqing Medical University, Chongqing, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Orthopedic Laboratory of Chongqing Medical University, Chongqing, China; Corresponding authors at: No. 1 Yi Xue Yuan Road, Yuzhong District, Chongqing 400016, China.Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Orthopedic Laboratory of Chongqing Medical University, Chongqing, China; Corresponding authors at: No. 1 Yi Xue Yuan Road, Yuzhong District, Chongqing 400016, China.Injectable biomaterials obtain increasing attention for the repair of bone defect due to the advantage of defect margin adaptability and easy handling. Our previous studies found that RADA16 peptide hydrogel could significantly promote bone formation. In order to improve the mechanical force, RADA16 peptide hydrogel was used to fill the porous calcium sulfate/nano-hydroxyapatite (CaSO4/HA) cement biomaterial for the fabrication of RADA16/CaSO4/HA composite biomaterial. Especially, RADA16 peptide hydrogel had the ability to slowly release growth factors for bone regeneration. Here, RADA16/CaSO4/HA composite biomaterials afforded the controlled and sustainable release of basic fibroblast growth factor (bFGF) for more than 32 days. Compared to RADA16/CaSO4/HA biomaterial, RADA16 + bFGF/CaSO4/HA composite biomaterial could further improve in vitro osteogenic differentiation of MC3T3 cells as evidenced by the increased expression of osteogenic markers (i.e. runt-related transcription factor 2 [Runx2], osteocalcin [OCN] and alkaline phosphatase [ALP]) and in vivo bone formation of femoral condyle defects which were confirmed by histological staining and micro-CT analyses. These indicate that bFGF-incorporated RADA16/CaSO4/HA can further improve bone formation through the controlled release of bFGF, which provides a novel injectable biomaterial design to specifically release growth factors for bone defects.http://www.sciencedirect.com/science/article/pii/S0264127522000909Bone defectRADA16/CaSO4/HAControlled releaseOsteogenic differentiationBone formation |
| spellingShingle | Bin He Muzi Zhang Lifeng Yin Zhengxue Quan Yunsheng Ou Wei Huang bFGF-incorporated composite biomaterial for bone regeneration Materials & Design Bone defect RADA16/CaSO4/HA Controlled release Osteogenic differentiation Bone formation |
| title | bFGF-incorporated composite biomaterial for bone regeneration |
| title_full | bFGF-incorporated composite biomaterial for bone regeneration |
| title_fullStr | bFGF-incorporated composite biomaterial for bone regeneration |
| title_full_unstemmed | bFGF-incorporated composite biomaterial for bone regeneration |
| title_short | bFGF-incorporated composite biomaterial for bone regeneration |
| title_sort | bfgf incorporated composite biomaterial for bone regeneration |
| topic | Bone defect RADA16/CaSO4/HA Controlled release Osteogenic differentiation Bone formation |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522000909 |
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