Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins
Mucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study the relationship between mucosal and systemic antibody responses, we took advantage of...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-09-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717311439 |
| _version_ | 1811278027021090816 |
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| author | Rasmus Iversen Omri Snir Maria Stensland José E. Kroll Øyvind Steinsbø Ilma R. Korponay-Szabó Knut E.A. Lundin Gustavo A. de Souza Ludvig M. Sollid |
| author_facet | Rasmus Iversen Omri Snir Maria Stensland José E. Kroll Øyvind Steinsbø Ilma R. Korponay-Szabó Knut E.A. Lundin Gustavo A. de Souza Ludvig M. Sollid |
| author_sort | Rasmus Iversen |
| collection | DOAJ |
| description | Mucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study the relationship between mucosal and systemic antibody responses, we took advantage of celiac disease patient samples for isolation of gut PCs as well as serum IgA and IgG reactive with a gluten-derived peptide or the autoantigen transglutaminase 2. Proteomic analysis of serum IgA revealed antigen-specific V-gene preferences, which matched those found in gut PCs. Further, gut PC CDR-H3 sequences were abundant in serum IgA but also detectable in serum IgG. Our data indicate that the same B cell clones that give rise to gut PCs also contribute to the serum antibody pool. However, serum IgA antibodies had a molecular composition distinct from that of IgA antibodies secreted in the gut, suggesting that individual B cell clones give rise to different PC populations. |
| first_indexed | 2024-04-13T00:28:26Z |
| format | Article |
| id | doaj.art-f2cc7373d9834077afa7fe78d8fb15b6 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-13T00:28:26Z |
| publishDate | 2017-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f2cc7373d9834077afa7fe78d8fb15b62022-12-22T03:10:33ZengElsevierCell Reports2211-12472017-09-0120102357236710.1016/j.celrep.2017.08.036Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell OriginsRasmus Iversen0Omri Snir1Maria Stensland2José E. Kroll3Øyvind Steinsbø4Ilma R. Korponay-Szabó5Knut E.A. Lundin6Gustavo A. de Souza7Ludvig M. Sollid8Centre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayBrain Institute, Federal University of Rio Grande do Norte, RN 59056-450 Natal, BrazilCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayCeliac Disease Center, Heim Pál Children’s Hospital, HU-1089 Budapest, HungaryCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayCentre for Immune Regulation and Department of Immunology, University of Oslo and Oslo University Hospital-Rikshospitalet, NO-0372 Oslo, NorwayMucosal antigens induce generation of lamina propria plasma cells (PCs) that secrete dimeric immunoglobulin A (IgA) destined for transport across the epithelium. In addition, blood contains monomeric IgA. To study the relationship between mucosal and systemic antibody responses, we took advantage of celiac disease patient samples for isolation of gut PCs as well as serum IgA and IgG reactive with a gluten-derived peptide or the autoantigen transglutaminase 2. Proteomic analysis of serum IgA revealed antigen-specific V-gene preferences, which matched those found in gut PCs. Further, gut PC CDR-H3 sequences were abundant in serum IgA but also detectable in serum IgG. Our data indicate that the same B cell clones that give rise to gut PCs also contribute to the serum antibody pool. However, serum IgA antibodies had a molecular composition distinct from that of IgA antibodies secreted in the gut, suggesting that individual B cell clones give rise to different PC populations.http://www.sciencedirect.com/science/article/pii/S2211124717311439proteomicsantibodiesplasma cellsmucosal immune systemautoimmunityceliac diseasemass spectrometrynext-generation sequencing |
| spellingShingle | Rasmus Iversen Omri Snir Maria Stensland José E. Kroll Øyvind Steinsbø Ilma R. Korponay-Szabó Knut E.A. Lundin Gustavo A. de Souza Ludvig M. Sollid Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins Cell Reports proteomics antibodies plasma cells mucosal immune system autoimmunity celiac disease mass spectrometry next-generation sequencing |
| title | Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins |
| title_full | Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins |
| title_fullStr | Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins |
| title_full_unstemmed | Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins |
| title_short | Strong Clonal Relatedness between Serum and Gut IgA despite Different Plasma Cell Origins |
| title_sort | strong clonal relatedness between serum and gut iga despite different plasma cell origins |
| topic | proteomics antibodies plasma cells mucosal immune system autoimmunity celiac disease mass spectrometry next-generation sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717311439 |
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