3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids
Six estuarine fulvic (FA) and humic acids (HA) were fractionated with reversed-phase high performance liquid chromatography in a water/acetonitrile gradient, with fluorescence detection. Differences between FA and HA chromatograms pointed to a higher incidence of hydrophobic components in the latter...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2006-01-01
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| Series: | Journal of the Brazilian Chemical Society |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000100017 |
| _version_ | 1819160413240033280 |
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| author | Sierra Marta M. D. Giovanela Marcelo Parlanti Edith Soriano-Sierra Eduardo J. |
| author_facet | Sierra Marta M. D. Giovanela Marcelo Parlanti Edith Soriano-Sierra Eduardo J. |
| author_sort | Sierra Marta M. D. |
| collection | DOAJ |
| description | Six estuarine fulvic (FA) and humic acids (HA) were fractionated with reversed-phase high performance liquid chromatography in a water/acetonitrile gradient, with fluorescence detection. Differences between FA and HA chromatograms pointed to a higher incidence of hydrophobic components in the latter. Excitation-emission matrix fluorescence diagrams of most fractions were similar to those of the bulk samples indicating that, in spite of their distinct polarities, the complexity and main spectral characteristics of the materials persisted. A primary group of fluorophores, which are present in most of the fractions, seems to be at the origin of the fluorescence properties of both kinds of HS. A bathochromic shift of the bulk solutions spectra in relation to the fraction emissions was attributed to a greater proximity of these fluorophores, possibly due to the formation of aggregates. Generally, the results reinforced concepts that humic macromolecules consist of assemblages of structurally similar building blocks. |
| first_indexed | 2024-12-22T16:56:03Z |
| format | Article |
| id | doaj.art-f2ce4deac2324474bfb83ddcb4aed1eb |
| institution | Directory Open Access Journal |
| issn | 0103-5053 1678-4790 |
| language | English |
| last_indexed | 2024-12-22T16:56:03Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Journal of the Brazilian Chemical Society |
| spelling | doaj.art-f2ce4deac2324474bfb83ddcb4aed1eb2022-12-21T18:19:28ZengSociedade Brasileira de QuímicaJournal of the Brazilian Chemical Society0103-50531678-47902006-01-011711131243D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acidsSierra Marta M. D.Giovanela MarceloParlanti EdithSoriano-Sierra Eduardo J.Six estuarine fulvic (FA) and humic acids (HA) were fractionated with reversed-phase high performance liquid chromatography in a water/acetonitrile gradient, with fluorescence detection. Differences between FA and HA chromatograms pointed to a higher incidence of hydrophobic components in the latter. Excitation-emission matrix fluorescence diagrams of most fractions were similar to those of the bulk samples indicating that, in spite of their distinct polarities, the complexity and main spectral characteristics of the materials persisted. A primary group of fluorophores, which are present in most of the fractions, seems to be at the origin of the fluorescence properties of both kinds of HS. A bathochromic shift of the bulk solutions spectra in relation to the fraction emissions was attributed to a greater proximity of these fluorophores, possibly due to the formation of aggregates. Generally, the results reinforced concepts that humic macromolecules consist of assemblages of structurally similar building blocks.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000100017RP-HPLCfulvic acidshumic acidsEEM fluorescence |
| spellingShingle | Sierra Marta M. D. Giovanela Marcelo Parlanti Edith Soriano-Sierra Eduardo J. 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids Journal of the Brazilian Chemical Society RP-HPLC fulvic acids humic acids EEM fluorescence |
| title | 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids |
| title_full | 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids |
| title_fullStr | 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids |
| title_full_unstemmed | 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids |
| title_short | 3D-fluorescence spectroscopic analysis of HPLC fractionated estuarine fulvic and humic acids |
| title_sort | 3d fluorescence spectroscopic analysis of hplc fractionated estuarine fulvic and humic acids |
| topic | RP-HPLC fulvic acids humic acids EEM fluorescence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000100017 |
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